Urea-specific enzyme electrode

Guilbault, George G.; Montalvo, Joseph G.

doi:10.1021/ja01036a083

Cited by 317 publications

(75 citation statements)

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“…Biosensors have attractive applications in human care, food industry, controlling the environment and for genetic applications. The greatest advantage of such biosensors are in their application being user friendly, having a fast response, high sensitivity, strong selectivity, and their practical use for real sampling [14][15][16][17]. The first oxygen sensor was invented by Leland C. Clark Jr in 1956, for oxygen detection in blood, water and other liquids [14].…”

Section: Biosensorsmentioning

confidence: 99%

“…At the end of 1967 glucose detection based on an independent and operational enzymatic electrode was developed [16]. The first investigation of potentiometric urea detection, based on urease with an ammonium selective membrane immobilized electrode, was published in 1969 [17]. In 1970, the pH ion selective field effect transistors electrodes were demonstrated [19].…”

Section: Biosensorsmentioning

confidence: 99%

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Synthesising Metal Oxide Materials and Their Composite Nanostructures for Sensing and Optoelectronic Device Applications

Khun¹

2015

Linköping Studies in Science and Technology. Dissertations

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Research on nanomaterials has been revolutionized in the last few years because of the attractive properties they have in comparison to the bulk phase of similar materials. These properties are physical, chemical, catalytic and optical. Among these nanomaterials, the metal oxide nanostructures have become of particular interest to scientists for the development of different optical, biochemical and biomedical nanodevices. In the present research work using the advantageous features of nanotechnology, high performance nanodevices for optoelectronics with a wide band gap compound nanostructure and highly sensitive sensor devices have been demonstrated. The nanotechnology is used to fabricate sensitive and precise nanodevices based on nanomaterials for the application of sensing.Among metal oxide nanostructures, ZnO, CuO and NiO are attractive materials because of their unique properties; their high surface area to volume ratio, their energy band gap of 3.37 eV, 1.2 eV and 3.7 eV, respectively, biocompatibility, high electron mobility, fast electron transfer rate and they are environmental-friendly in many applications. When used in sensor devices, nanomaterials have indicated high selectivity for possible use to detect the various analytes even in small volumes. Metal oxide nanostructures have shown to be good for optoelectronic nanodevices because of their electrical characteristics, high optical absorption and low-processing temperature.In this thesis, the synthesis of different morphologies of metal oxide semiconductor nanostructures and their composite using the hydrothermal method are demonstrated for various applications. This thesis is divided into three parts:In the first part of this research work, the fabrication of well-aligned ZnO nanorods using different concentrations of composite seed layer of inorganic and organic materials when using the hydrothermal growth method is presented. The effect of the composite seed layer on the i Abstract alignment, density and optical properties of the grown ZnO nanorods is investigated (paper I).Utilizing the advantage of ZnO nanostructure, a comparative study of ZnO nanorods and thin films for chemical and biosensing application was carried out. The ZnO nanorods and thin films were functionalized with strontium ionophore membrane, immobilized the galactose oxidase and lactate oxidase for determining the strontium ions, D-galactose and L-lactic acid, respectively (paper II).In the second part, the effects of different urea concentrations on the morphology of CuO nanostructures is studied as described in paper III. Moreover, CuO nanoflowers were functionalized with cadmium ion ionophore for the detection of Cd ions, while CuO nanosheets were grown by the low temperature growth method and were used for the development of a nonenzymatic glucose sensor, respectively (Paper IV).In the last part of this thesis, composite nanostructures of CuO/ZnO and NiO/ZnO were applied to develop dopamine sensor and fast sensitive UV photodetector, respectively. A nanohybrid of C...

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Section: Biosensorsmentioning

confidence: 99%

Section: Biosensorsmentioning

confidence: 99%

Synthesising Metal Oxide Materials and Their Composite Nanostructures for Sensing and Optoelectronic Device Applications

Khun¹

2015

Linköping Studies in Science and Technology. Dissertations

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“…As primeiras imobilizações constavam de membranas semipermeáveis, acopladas a eletrodos clássicos, como o de pH e o de Clark. 1,4,29 Seguidamente, os eletrodos sólidos e metálicos foram então utilizados, como os de pastas de carbono, carbono vítreo, ouro e platina. 2,3 Para os arranjos ópticos, diversos tipos de fibras têm sido empregados, com distintos formatos, fazendo uso das mesmas técnicas de imobilização empregadas nos sensores eletroquímicos, com poucas modificações.…”

Section: Materiais Empregadosunclassified

“…1,2 Quando este material é uma enzima, estes sensores são denominados biossensores enzimáticos, que, entre outros, podem fazer uso da atividade enzimática como sinal analítico a ser monitorado. 3,4 Dentre os variados tipos, merecem destaque aqueles em que o monitoramento do analito é baseado no princípio de inibição da enzima, na qual a atividade é medida antes e após a inibição. Este processo é efetuado pela exposição da enzima a um inibidor especí-fico, por um determinado tempo, sendo que a porcentagem de inibição sofrida pela enzima está quantitativamente relacionada com a concentração deste agente inibidor.…”

Section: Introductionunclassified

Biossensores baseados no processo de inibição enzimática

Marques¹,

Yamanaka²

2008

Quím. Nova

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Recebido em 3/5/07; aceito em 30/11/07; publicado na web em 4/9/08 BIOSENSORS BASED ON THE ENZYMATIC INHIBITION PROCESS. Fast, selective, reproducible and reliable detections have been carried out by using enzymatic biosensors in several areas. The enzymatic biosensors based on the inhibition represent an important role in analytical chemistry. Enzymes like cholinesterases, peroxidases, tyrosinases, etc. have been immobilized on electrochemical and optical transducers and the enzymatic activity decreasing in the presence of the inhibitor is related with its concentrations. This article presents a review on the enzymes used on the construction of these sensors, emphasizing the respective applications.

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“…Their versatile chemical selectivity could easily be tuned with lipophilic ionophore [9]. The first potentiometric enzyme sensor was reported in 1969 [10] since then fast advances in the field of potentiometric sensing have been demonstrated e.g. [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Sensitive Potentiometric Cholesterol Biosensor Based on Co₃O₄ Interconnected Nanowires

Ibupoto¹,

Elhag²,

Nur³

et al. 2014

Electroanalysis

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Urea-specific enzyme electrode

Cited by 317 publications

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Synthesising Metal Oxide Materials and Their Composite Nanostructures for Sensing and Optoelectronic Device Applications

Synthesising Metal Oxide Materials and Their Composite Nanostructures for Sensing and Optoelectronic Device Applications

Biossensores baseados no processo de inibição enzimática

Fabrication of Sensitive Potentiometric Cholesterol Biosensor Based on Co₃O₄ Interconnected Nanowires

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Urea-specific enzyme electrode

Cited by 317 publications

References 0 publications

Synthesising Metal Oxide Materials and Their Composite Nanostructures for Sensing and Optoelectronic Device Applications

Synthesising Metal Oxide Materials and Their Composite Nanostructures for Sensing and Optoelectronic Device Applications

Biossensores baseados no processo de inibição enzimática

Fabrication of Sensitive Potentiometric Cholesterol Biosensor Based on Co3O4 Interconnected Nanowires

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Fabrication of Sensitive Potentiometric Cholesterol Biosensor Based on Co₃O₄ Interconnected Nanowires