Urea, salts, and Tween 20 influence on adsorption of IgG and Leishmania rNTPDase2 to nitrocellulose

Castro, Raissa Barbosa de; Souza, Anna Cláudia Alves de; Pavione, Nancy da Rocha Torres; Moraes, João Victor Badaró de; Ribeiro, Isadora Cunha; Agripino, Joice de Melo; Bressan, Gustavo Costa; Vasconcellos, Raphael de Souza; Silva-Júnior, Abelardo; Fietto, Juliana Lopes Rangel

doi:10.1016/j.ab.2022.114648

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2023

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“…1 chromatography paper is the most-used paper substrate for paper-based microfluidics, while nitrocellulose paper is more widely used for lateral flow immunoassays 63,64 for its better protein adsorption ability. 65,66 As an alternative, office paper, a substrate of commercial interest, is accessible but less attractive for technical paper, 67,68 because the high amount of calcium carbonate (CaCO 3 ) present may interfere with the pH. 69 Using Whatman No.…”

Section: Papermentioning

confidence: 99%