Urea biosensor based on PANi(urease)-Nafion®/Au composite electrode

Luo, Yung-Chien; Do, Jing-Shan

doi:10.1016/j.bios.2003.11.028

Cited by 157 publications

(45 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, these materials present very attractive advantages such as a low cost of purification or synthesis and their biocompatibility. Furthermore, the bi-dimensional structure of LDHs with the general formula: [3][4][5][6][7][8][9][10]. Therefore, this kind of materials can be considered very attractive for new hybrid biomaterials development and for functionalized surfaces for ionic and biologic detection [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Conductor's polymers and magnetic nanoparticles were used in the fabrication of various types of HRP-based biosensors [1,2]. To stabilize the immobilized enzyme in the matrix of film, glutaraldehyde (GA) is usually employed as a bifunctional agent to cross-link enzyme molecules [3][4][5][6], but the cross-linking efficiency under standard conditions is not always satisfactory [6,7], which results in the lower sensitivity and poor stability of the resulting biosensor. It was established that cationic clays and especially anionic ones or layered double hydroxides were considered as a new class of materials with a high trapping potential of molecules of different sizes and that they form hybrid materials.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application

Baccar¹,

Hafaiedh

2011

International Journal of Electrochemistry

View full text Add to dashboard Cite

We present a new biosensor for hydrogen peroxide (H 2 O 2 ) detection. The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides-(LDH-) modified gold surface. The hydrotalcite LDH (Mg 2 Al) was prepared by coprecipitation in constant pH and in ambient temperature. The immobilization of the peroxidase on layered hybrid materials was realized via electrostatic adsorption autoassembly process. The detection of hydrogen peroxide was successfully observed in PBS buffer with cyclic voltammetry and the chronoamperometry techniques. A limit detection of 9 µM of H 2 O 2 was obtained with a good reproducibility. We investigate the sensitivity of our developed biosensor for H 2 O 2 detection in raw milk.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application

Baccar¹,

Hafaiedh

2011

International Journal of Electrochemistry

View full text Add to dashboard Cite

show abstract

“…Typically, its physiological levels range between 2.5 mM and 8.6 mM in serum depending on the daily protein ingestion (Beers and Berkow, 1999) and its analysis carried out frequently in various laboratories (Luo and Do, 2004). Small handheld electrochemical urea meters for use in medicine capable of intermittent ''spot'' urea monitoring are being manufactured by Urea assay Kit (ab83362) (Abcam plc UK).…”

Section: Mnppf For Urea Sensormentioning

confidence: 99%

Polymer thin films embedded with metal nanoparticles for electrochemical biosensors applications

Prakash

Chakrabarty

Singh

et al. 2013

Biosensors and Bioelectronics

182

View full text Add to dashboard Cite

“…For this reason, considerable efforts have recently been devoted toward the adaptation of PANI to work in neutral or higher pH conditions. To maintain PANI as electrochemically active at higher pH, self-doping 14,15 and external doping 16 have been used as the preferred approaches. 17 Self-doped structures obtained by direct sulfonation of PANI usually enhance its electroactivity, but at the cost of a decrease in conductivity.…”

Section: Introductionmentioning

confidence: 99%

Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite

An¹,

Yoon²,

Das³

2015

IJN

View full text Add to dashboard Cite

An electrochemical biosensor based on sulfonated graphene/polyaniline nanocomposite was developed for urea analysis. Oxidative polymerization of aniline in the presence of sulfonated graphene oxide was carried out by electrochemical methods in an aqueous environment. The structural properties of the nanocomposite were characterized by Fourier-transform infrared, Raman spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy techniques. The urease enzyme-immobilized sulfonated graphene/polyaniline nanocomposite film showed impressive performance in the electroanalytical detection of urea with a detection limit of 0.050 mM and a sensitivity of 0.85 (μA · cm −2 ·mM −1 . The biosensor achieved a broad linear range of detection (0.12–12.3 mM) with a notable response time of approximately 5 seconds. Moreover, the fabricated biosensor retained 81% of its initial activity (based on sensitivity) after 15 days of storage at 4°C. The ease of fabrication coupled with the low cost and good electrochemical performance of this system holds potential for the development of solid-state biosensors for urea detection.

show abstract

Urea biosensor based on PANi(urease)-Nafion®/Au composite electrode

Cited by 157 publications

References 21 publications

Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application

Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application

Polymer thin films embedded with metal nanoparticles for electrochemical biosensors applications

Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite

Contact Info

Product

Resources

About