Urea and creatinine detection on nano-laminated gold thin film using Kretschmann-based surface plasmon resonance biosensor

Menon, P. Susthitha; Said, Fairus Atida; Mei, Gan Siew; Berhanuddin, Dilla Duryha; Umar, Akrajas Ali; Shaari, Sahbudin; Majlis, Burhanuddin Yeop

doi:10.1371/journal.pone.0201228

Cited by 68 publications

(35 citation statements)

References 33 publications

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“…As the refractive index at the sensing region changed due to increase in urea concentration, shift in resonance angle were recorded in SPR spectra. Sensorgram, linearity, repeatability and sensitivity analyses of the 50 nm-thick nanolaminated gold film sensor for urea detection were investigated based on the SPR spectra obtained (Menon et al 2018;Said et al 2017).…”

Section: Experimental Methodsmentioning

confidence: 99%

High Sensitivity Au-based Kretschmann Surface Plasmon Resonance Sensor for Urea Detection

Menon¹,

Said²,

Gan³

et al. 2019

JSM

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Kretschmann-based Surface Plasmon Resonance (SPR) optical sensor was applied to detect the presence of kidney wastes such as urea in solutions. To enhance the sensitivity of the SPR sensor, nanolaminated gold film (thickness of 50 nm) was used. In this work, the SPR response to urea in various concentrations were measured and investigated using optical wavelengths of 670 nm and 785 nm. The signals were compared between pure urea solution versus mixed solution in the presence of the 0.227 µmol urease enzyme. The proposed mixed solution is to eliminate doping and gel entrapment process for enzyme immobilization in conventional method. Angular interrogation technique was used to measure the sensor performance in urea detection using pure and mixed solutions with urea concentration of 0-800 mM. Upon exposure to mixed solution using 785 nm optical wavelength, the nanolaminated gold film exhibited higher SPR sensitivity as much as 7.8 M-1 than a pure urea solution (1.4 M-1). The coupling activity between urea and urease molecules in the mixed solution near the nanolaminated gold film surface lead to sensitivity enhancement. Angle shifting of mixed solution on 50 nm-thick nanolaminated gold film using 670 nm optical wavelength was greater by ~50% compared to 785 nm. Sensorgram data shows a steady and linear increment in SPR incident angle shifting when urea concentration increased. To the best of our knowledge, this is the first time that Kretschmann-based SPR has been used for urea sensing at 670 nm and 785 nm optical wavelengths.

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Section: Experimental Methodsmentioning

confidence: 99%

High Sensitivity Au-based Kretschmann Surface Plasmon Resonance Sensor for Urea Detection

Menon¹,

Said²,

Gan³

et al. 2019

JSM

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“…Prior to the deposition and characterization of an SPR biosensor, ZnO is optimized using numerical methods to achieve a high-performance sensor. This can be achieved by simulation of the SPR curve with different thicknesses of the ZnO layer using Finite Difference Time-Domain (FDTD) method from Lumerical [55][56][57]. As the thickness of the ZnO increases, the FWHM and resonance angle of the SPR signal becomes larger which would affect sensor reliability and sensitivity.…”

Section: Performance Parameter Of Spr Biosensormentioning

confidence: 99%

ZnO for performance enhancement of surface plasmon resonance biosensor: a review

Mei

Menon

Hegde³

2020

Mater. Res. Express

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This paper reviews Kretschmann-based SPR sensor utilizing ZnO thin films and nanostructures for performance enhancement. The advancement in surface plasmon resonance technology relies on low-cost, high sensitivity and high selectivity sensor. Metal oxide has been incorporated in SPR sensor to be used for detection of biological and chemical compounds. ZnO as one of the metal oxides is an attractive material due to its unique physical and optical properties. Numerous techniques for fabrication and characterization of ZnO on SPR gold substrate have been studied. The mechanism for gas and biomolecules detection depends on their interaction with ZnO surface, which is mainly attributed to the high isoelectric point of ZnO. There are several types of ZnO nanostructures which have been employed for SPR application based on the Kretschmann configuration. In future, the thin film and nanostructures of ZnO have potential applications for miniature design, robust, high sensitivity, and low-cost portable type of SPR biosensor to be used for on-site testing in real-time and label-free manner.

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“…The combination of different materials and thicknesses have also been used to investigate the effectiveness of the SPR Jamil et al 2018;Mohamad et al 2019;Said et al 2016;Tarumaja et al 2016). This K-SPR approach gives better benefits as compared to other label-free approaches (Menon et al 2018). The benefits of utilizing the Kretschmann-based SPR sensor with Au-Cr layers are high sensitivity, more user friendly, and lower cost needed as well as label free and real time detection.…”

Section: Introductionmentioning

confidence: 99%

“…Numerical simulation using various software have been constantly used in order to prove the performances of new sensor designs (Maheran et al 2014;Menon et al 2010;Haroon et al 2013). In our previous work, finite difference time domain (FDTD) numerical simulation from Lumerical was utilized for Krestchmann-based SPR sensing (Gan et al 2018;Jamil et al 2018aJamil et al , 2018bJamil et al , 2017aJamil et al , 2017bMenon et al 2018;Said et al 2017Said et al , 2015.…”

Section: Introductionmentioning

confidence: 99%

Refractive Index and Sensing of Glucose Molarities determined using Au-Cr K-SPR at 670/785 nm Wavelength

Menon¹,

Mulyanti²,

Jamil³

et al. 2019

JSM

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In this paper, we determine the optical refractive indices of different molarities of glucose using nano-laminated gold/chromium (Au-Cr) thin film via Kretschmann-based Surface Plasmon Resonance (K-SPR) sensing with angular interrogation. The nano-laminated Au-Cr K-SPR sensor detects the glucose presence in low-and high-concentration of 4-12 mmol/L and 55-277 mmol/L, respectively, under the exposure of 670 nm and 785 nm optical wavelengths. The experimental results showed that the minimum limit of detection (LOD) of Au-Cr K-SPR is 4 mmol/L and the glucose sensor sensitivities are in average of 3.41 o /M and 2.73 o /M at 670 nm and 785 nm optical wavelength, respectively. Stable sensitivity for each concentration also shown from the sensorgram results, indicates the stable performance of nanolaminated Au-Cr SPR sensor to detect glucose in the range from mmol/L up to dmol/L. Values of refractive indices for glucose molarities obtained are 1.33187 (4 mmol/L) and 1.3191 (4 mmol/L) at 670 and 785 nm wavelength, respectively. These RI values are beneficial for numerical simulation of glucose sensors using nano-laminated Au-Cr thin films which have been reported for the first time. The sensor can be eventually deployed in integrated photonic sensing devices comprising of multiple analyte detection for lab-on-chip (LoC) and point-of care (PoC) applications.

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Urea and creatinine detection on nano-laminated gold thin film using Kretschmann-based surface plasmon resonance biosensor

Cited by 68 publications

References 33 publications

High Sensitivity Au-based Kretschmann Surface Plasmon Resonance Sensor for Urea Detection

High Sensitivity Au-based Kretschmann Surface Plasmon Resonance Sensor for Urea Detection

ZnO for performance enhancement of surface plasmon resonance biosensor: a review

Refractive Index and Sensing of Glucose Molarities determined using Au-Cr K-SPR at 670/785 nm Wavelength

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