Background: In 2016, a large chain supermarket opened in the Englewood community of Chicago, IL – a low-income African American community. The development of this supermarket was supported, in part, by the Healthy Food Financing Initiative (HFFI) – a federal initiative to expand access to healthy foods in food deserts. The aim of this study was to examine changes to food and beverage availability and marketing in Englewood’s existing food stores after the supermarket’s opening. Methods: A quasi-experimental study was conducted from 2016 – 2018. Trained fieldworkers audited all small grocery and limited-service stores (e.g., convenience stores, liquor stores, pharmacies, dollar/discount stores) located within one-square mile of the new supermarket and a one-square mile area of a demographically comparable community in Chicago that also lacked a supermarket. All stores in the one-square mile area were audited at three time points: before (2016) and after (2017 and 2018) the supermarket opened. Extensive data on availability and marketing were collected for staple food items, snacks, and beverages. Difference-in-differences (DID) regression models were used to identify significant differences between the intervention and comparison communities in the changes in food and beverage availability and marketing. Results: Of the 78 stores audited at baseline, 71.8% were limited-service stores, and 85.9% accepted Supplemental Nutrition Assistance Program (SNAP) benefits. The availability of healthy food and beverage options in existing food stores was limited at baseline and both follow-up periods. Stores in the intervention community offered, on average, < 3 fresh vegetable options and < 2 fresh fruit options at all three time periods. DID regression models revealed a significant increase in 1) the percentage of stores in the intervention community offering regular cheese and promoting salty snacks at check-out from 2016 – 2017 and 2) the percentage of stores in the comparison community with interior store promotions for other sweetened beverages from 2016 – 2018. Conclusions: Minimal changes in food and beverage availability and marketing occurred one and two years after the opening of the new supermarket. However, the wide range of staple food items offered by the supermarket expanded healthy food retail in Chicago’s Englewood community.