Uranium deposition in bones of Wistar rats associated with skeleton development

Rodrigues, Giovani Antonio; Arruda-Neto, João D T; Pereira, Rosa Maria Rodrigues; Kleeb, Silvia Regina; Geraldo, L.P.; Primi, M.C.; Takayama, Lilian; Rodrigues, T. E.; Cavalcante, Guacyara Tenório; Genofre, Godofredo C.; Semmler, R.; Nogueira, Guilherme de Paula; Fontes, E.M.

doi:10.1016/j.apradiso.2013.07.033

Cited by 20 publications

(6 citation statements)

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“…Sanchez et al (2006) reported that uranium accumulation in the bones of exposed fetuses was higher than in the fetal kidneys, an organ which is generally considered to be a primary target of uranium toxicity (Arzuaga et al, 2010;ATSDR, 2013). Uranium is also known to accumulate in areas of bone growth (Neuman et al, 1948;Rodrigues et al, 2013;Rowland and Farnham, 1969), where active calcification is occurring Durbin and Wrenn, 1975). Furthermore, ex-vivo studies using mouse preimplantation embryos (Kundt et al, 2000), and in vivo studies using the nonmammalian vertebrates Danio rerio and Xenopus laevis have shown that uranium exposure reduces embryo growth (Bourrachot et al, 2008;Mitchell et al, 2005).…”

Section: Discussionmentioning

confidence: 96%

“…In humans and animals, uranium deposits in the skeleton in a dose-and timedependent fashion (Arruda-Neto et al, 2004;Lariviere et al, 2013;Pellmar et al, 1999;Prado et al, 2008) and uranium accumulation in bones occurs at a higher rate in young individuals (Lariviere et al, 2007;Rodrigues et al, 2013). Thus, bone is considered to be the most significant, long term deposit of uranium.…”

mentioning

confidence: 98%

“…Within the osseous tissue, uranium accumulates in areas of active bone formation, specifically the calcifying zones of skeletal cartilage, where it can mimic and replace calcium (Ca 2+ ) (ATSDR, 2013; Rodrigues et al, 2013).…”

mentioning

confidence: 99%

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Modes of action associated with uranium induced adverse effects in bone function and development

2015

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Section: Discussionmentioning

confidence: 96%

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confidence: 98%

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Modes of action associated with uranium induced adverse effects in bone function and development

2015

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“…Bones are the secondary target organ of uranium toxicity. The majority of absorbed uranium, which mimics calcium, is stored in the bones, and could induce death of bone cells (Rodrigues et al, 2013). It is still unclear if the mutagenic and carcinogenic affects are a result of the chemical or radiological effects of uranium, but it is believed that these effects are primarily due to the latter.…”

Section: Carcinogenicitymentioning

confidence: 99%

Human Health and Environmental Uranium

Patočka¹

2014

Mil. Med. Sci. Lett.

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Uranium from the environment enters the human body by ingestion with food and drink and by inhalation of respirable airborne uranium-containing dust particles or aerosols. A 70 kg, non-occupationally exposed 'Reference Man' living in Europe or in the United States has an estimated total body uranium content of about 22 micrograms. Uranium is absorbed from the intestine or the lungs, enters the bloodstream, and is rapidly deposited in the tissues, predominantly kidney and bone, or excreted into the urine. In the bloodstream, uranium is associated with red cells, and its clearance is relatively rapid. Renal toxicity is a major adverse effect of uranium, but the metal has toxic effects on the cardiovascular system, liver, muscle, and nervous system as well. Any possible direct risk of cancer or other chemical-or radiation-induced health detriments from uranium deposited in the human body is probably less than 0.005% in contrast to an expected indirect risk of 0.2% to 3% through inhaling the radioactive inert gas radon, which is produced by the decay of environmental uranium-238 in rocks and soil and is present in materials that are used to build dwellings and buildings where people live and work.

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“…SSNTD have been used for multiple applications since their first description by Young (1958), including radon and uranium dosimetry (e.g., Stegnar et al, 2013; Hadad et al, 2013), personal neutron dosimetry (e.g., Djeffal et al, 1997; Saint Martin et al, 2011), and detection of cosmic rays (e.g., Dey et al, 2011). One of the outstanding applications includes mapping heavy particle emitters in mineral and biological materials (e.g., Armijo and Rosenbaum 1967; Bersina et al, 1995; Thellier et al, 2001, Rodrigues et al, 2013; Chauhan & Chauhan, 2014). Indeed, the track density analysis on a detector put in contact with a tissue section that contains a heavy particle emitter allows determination of the spatial distribution of the element in the sample.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Observation of Cells and Nuclear Tracks from the Boron Neutron Capture Reaction by UV-C Sensitization of Polycarbonate

Portu

Rossini²,

Thorp

et al. 2015

Microsc Microanal

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The distribution of boron in tissue samples coming from boron neutron capture therapy protocols can be determined through the analysis of its autoradiography image on a nuclear track detector. A more precise knowledge of boron atom location on the microscopic scale can be attained by the observation of nuclear tracks superimposed on the sample image on the detector. A method to produce an "imprint" of cells cultivated on a polycarbonate detector was developed, based on the photodegradation properties of UV-C radiation on this material. Optimal conditions to generate an appropriate monolayer of Mel-J cells incubated with boronophenylalanine were found. The best images of both cells and nuclear tracks were obtained for a neutron fluence of 1013 cm-2, 6 h UV-C (254 nm) exposure, and 4 min etching time with a KOH solution. The imprint morphology was analyzed by both light and scanning electron microscopy. Similar samples, exposed to UV-A (360 nm) revealed no cellular imprinting. Etch pits were present only inside the cell imprints, indicating a preferential boron uptake (about threefold the incubation concentration). Comparative studies of boron absorption in different cell lines and in vitro evaluation of the effect of diverse boron compounds are feasible with this methodology.

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Uranium deposition in bones of Wistar rats associated with skeleton development

Cited by 20 publications

References 16 publications

Modes of action associated with uranium induced adverse effects in bone function and development

Modes of action associated with uranium induced adverse effects in bone function and development

Human Health and Environmental Uranium

Simultaneous Observation of Cells and Nuclear Tracks from the Boron Neutron Capture Reaction by UV-C Sensitization of Polycarbonate

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