Uralsaponins M–Y, Antiviral Triterpenoid Saponins from the Roots of <i>Glycyrrhiza uralensis</i>

Song, Wei; Si, Longlong; Ji, Shuai; Wang, Han; Fang, Xiaoyun; Yu, Liyan; Li, Ren-Yong; Liang, Lina; Zhou, Demin; Ye, Min

doi:10.1021/np500253m

Cited by 97 publications

(74 citation statements)

References 25 publications

(58 reference statements)

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“…Several saponins have exhibited potent cytotoxic [33], antiproliferative [34], antifungal [35], anti-inflammatory [36], and antiviral activity [37], which indicates a significant potential for drug development. One particulary promising property of spirostan saponins is their anticancer activity.…”

Section: Discussionmentioning

confidence: 99%

Apoptotic and autophagic cell death induced by glucolaxogenin in cervical cancer cells

Sánchez-Sánchez

Escobar²,

Sandoval‐Ramírez

et al. 2015

Apoptosis

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The antiproliferative and cytotoxic activity of glucolaxogenin and its ability to induce apoptosis and autophagy in cervical cancer cells are reported. We ascertained that glucolaxogenin exerts an inhibitory effect on the proliferation of HeLa, CaSki and ViBo cells in a dose-dependent manner. Analysis of DNA distribution in the cell-cycle phase of tumor cells treated with glucolaxogenin suggests that the anti-proliferative activity of this steroid is not always dependent on the cell cycle. Cytotoxic activity was evaluated by detection of the lactate dehydrogenase enzyme in supernatants from tumor cell cultures treated with the steroid. Glucolaxogenin exhibited null cytotoxic activity. With respect to the apoptotic activity, the generation of apoptotic bodies, the presence of active caspase-3 and annexin-V, as well as the DNA fragmentation observed in all tumor lines after treatment with glucolaxogenin suggests that this compound does indeed induce cell death by apoptosis. Also, a significantly increased presence of the LC3-II, LC3 and Lamp-1 proteins was evidenced with the ultrastructural existence of autophagic vacuoles in cells treated with this steroidal glycoside, indicating that glucolaxogenin also induces autophagic cell death. It is important to note that this compound showed no cytotoxic effect and did not affect the proliferative capacity of mononuclear cells obtained from normal human peripheral blood activated by phytohaemagglutinin. Thus, glucolaxogenin is a compound with anti-proliferative properties that induces programmed cell death in cancer cell lines, though it is selective with respect to normal lymphocytic cells. These findings indicate that this glycoside could have a selective action on tumor cells and, therefore, be worthy of consideration as a therapeutic candidate with anti-tumor potential.

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Section: Discussionmentioning

confidence: 99%

Apoptotic and autophagic cell death induced by glucolaxogenin in cervical cancer cells

Sánchez-Sánchez

Escobar²,

Sandoval‐Ramírez

et al. 2015

Apoptosis

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“…19,20 The CellTiter-Glo screening, an assay monitoring cell proliferation, was utilized to screen and exclude compounds with significant toxicity toward MDCK cells. The CPE screening, an assay for measuring the damage to host cells during viral invasion, was utilized to identify compounds, which display a reduction of the cytopathic effect on influenza A/WSN/33 virus.…”

Section: Antiviral Effects Of 1e14 Against H1n1 Virusmentioning

confidence: 99%

Truncateols A–N, new isoprenylated cyclohexanols from the sponge-associated fungus Truncatella angustata with anti-H1N1 virus activities

Zhao

Liu

et al. 2015

Tetrahedron

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“…Tu). Authentic saponins, including ginsenosides Rb1, Rb2, Rb3, Rc, Rd, Re, Rf, Rg1, Rg2, Rg3, Rg5, Rg6, Rh1, Rh2, Rk1, Ro, and F4, pseudoginsenoside F11, notoginsenoside R2, astragalosides II, III, and IV, isoastragaloside II, glycyrrhizic acid, and 18 β -glycyrrhetic acid were supplied by Chengdu Must Bio-technology Co., Ltd. (Chengdu, Sichuan, China), whilst uralsaponins C and F, 22 β -acetoxyglycyrrhizin, and licorice-saponins E2, G2, J2, H2, B2, and A3 were kindly provided by Prof. Min Ye (Peking University, Beijing, China)55. Reference flavonoids, such as (3 R )-(+)-isomucronulatol-2′- O - β -d-glucopyranoside, (3 R )-(−)-isomucronulatol-7- O - β -d-apiofuranosyl(1 → 2)- β -d-glucopyranoside, apigenin-6,8-di- C - β -d-glucopyranoside, neoisoliquiritin, calycosin, calycosin-7- O - β -d-glupyrancoside, liquiritigenin, liquiritin, isoliquiritigenin, isoliquiritin, isoliquiritin apioside, liquiritin apioside, licuraside, ononin, formononetin, and 7,4′-dihydroxyflavone were previously purified and identified from BYD in our laboratory1617, whereas three diterpenes, i.e., anhydrocinnzeylanol, anhydrocinnzeylanine, and cinnzeylanine were purified and identified from Cinnamomi Cortex56.…”

Section: Methodsmentioning

confidence: 99%

An Integrated Strategy for Global Qualitative and Quantitative Profiling of Traditional Chinese Medicine Formulas: Baoyuan Decoction as a Case

Guo

Song

et al. 2016

Sci Rep

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Clarification of the chemical composition of traditional Chinese medicine formulas (TCMFs) is a challenge due to the variety of structures and the complexity of plant matrices. Herein, an integrated strategy was developed by hyphenating ultra-performance liquid chromatography (UPLC), quadrupole time-of-flight (Q-TOF), hybrid triple quadrupole-linear ion trap mass spectrometry (Qtrap-MS), and the novel post-acquisition data processing software UNIFI to achieve automatic, rapid, accurate, and comprehensive qualitative and quantitative analysis of the chemical components in TCMFs. As a proof-of-concept, the chemical profiling of Baoyuan decoction (BYD), which is an ancient TCMF that is clinically used for the treatment of coronary heart disease that consists of Ginseng Radix et Rhizoma, Astragali Radix, Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle, and Cinnamomi Cortex, was performed. As many as 236 compounds were plausibly or unambiguously identified, and 175 compounds were quantified or relatively quantified by the scheduled multiple reaction monitoring (sMRM) method. The findings demonstrate that the strategy integrating the rapidity of UNIFI software, the efficiency of UPLC, the accuracy of Q-TOF-MS, and the sensitivity and quantitation ability of Qtrap-MS provides a method for the efficient and comprehensive chemome characterization and quality control of complex TCMFs.

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Uralsaponins M–Y, Antiviral Triterpenoid Saponins from the Roots of Glycyrrhiza uralensis

Cited by 97 publications

References 25 publications

Apoptotic and autophagic cell death induced by glucolaxogenin in cervical cancer cells

Apoptotic and autophagic cell death induced by glucolaxogenin in cervical cancer cells

Truncateols A–N, new isoprenylated cyclohexanols from the sponge-associated fungus Truncatella angustata with anti-H1N1 virus activities

An Integrated Strategy for Global Qualitative and Quantitative Profiling of Traditional Chinese Medicine Formulas: Baoyuan Decoction as a Case

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