Uracil influences quorum sensing and biofilm formation in <i>Pseudomonas aeruginosa</i> and fluorouracil is an antagonist

Ueda, Akihiro; Attila, Can; Whiteley, Marvin; Wood, Thomas K.

doi:10.1111/j.1751-7915.2008.00060.x

Cited by 142 publications

(114 citation statements)

References 42 publications

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“…In addition, two proteins GuaB and Upp were detected to be up-expressed in L. monocytogenes biofilm in this study. GuaB that converts IMP into GMP was important for the production of Mycobacterium avium biofilm [22], and Upp that catalyzes uracil to UMP was required to biofilm development in Pseudomonas aeruginosa through control of quorumsensing (QS) pathways [21]. Upp was aslo found to be induced by glucose starvation in L. monocytogenes biofilm [8].…”

Section: Identification Of Proteins Differentially Expressed Under Bimentioning

confidence: 99%

Carbon Catabolite Control is Important for Listeria monocytogenes Biofilm Formation in Response to Nutrient Availability

et al. 2012

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The foodborne pathogen Listeria monocytogenes has the ability to develop biofilm in food-processing environment, which becomes a major concern for the food safety. The biofilm formation is strongly influenced by the availability of nutrients and environmental conditions, and particularly enhanced in poor minimal essential medium (MEM) containing glucose rather than in rich brain heart infusion (BHI) broth. To gain better insight into the conserved protein expression profile in these biofilms, the proteomes from biofilm- and planktonic-grown cells from MEM with 50 mM glucose or BHI were compared using two-dimensional polyacrylamide gel electrophoresis followed by MALDI-TOF/TOF analysis. 47 proteins were successfully identified to be either up (19 proteins) or down (28 proteins) regulated in the biofilm states. Most (30 proteins) of them were assigned to the metabolism functional category in cluster of orthologous groups of proteins. Among them, up-regulated proteins were mainly associated with the pentose phosphate pathway and glycolysis, whereas a key enzyme CitC involved in tricarboxylic acid cycle was down-regulated in biofilms compared to the planktonic states. These data implicate the importance of carbon catabolite control for L. monocytogenes biofilm formation in response to nutrient availability.

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Section: Identification Of Proteins Differentially Expressed Under Bimentioning

confidence: 99%

Carbon Catabolite Control is Important for Listeria monocytogenes Biofilm Formation in Response to Nutrient Availability

et al. 2012

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“…From a screen of P. aeruginosa biofilm mutants, uracil was determined to act as a positive signal for biofilm formation, and 5-FU was shown to be effective in inhibiting this signaling, thereby repressing biofilm formation, significantly reducing QS phenotypes (10 M 5-FU reduced elastase activity by 86%, eliminated pyocyanin production, reduced rhamnolipid production by 87%, eliminated swarming, and eliminated Pseudomonas quinolone signal production), and reducing pathogenicity (5-FU increased barley germination) (48). This reduction of P. aeruginosa pathogenicity by 5-FU was rediscovered by Imperi et al (49) 4 years later, when they demonstrated that 5-fluorocytosine, which they showed is converted to 5-FU for its activity, also reduces pyoverdine, PrpL protease, and exotoxin in P. aeruginosa.…”

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confidence: 99%

Resistance to Quorum-Quenching Compounds

García‐Contreras

Maeda

Wood

2013

Appl Environ Microbiol

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c Bacteria have the remarkable ability to communicate as a group in what has become known as quorum sensing (QS), and this trait has been associated with important bacterial phenotypes, such as virulence and biofilm formation. Bacteria also have an incredible ability to evolve resistance to all known antimicrobials. Hence, although inhibition of QS has been hailed as a means to reduce virulence in a manner that is impervious to bacterial resistance mechanisms, this approach is unlikely to be a panacea. Here we review the evidence that bacteria can evolve resistance to quorum-quenching compounds. Infectious diseases are the leading cause of death (1), and all antibiotics fail (2); therefore, it is imperative to develop novel ways to fight microbial infections. Here we review the use of chemicals that interfere with cell communication and investigate the likelihood that bacteria will evolve resistance to these compounds.QS and QQ. Bacteria use secreted chemicals as signals for a variety purposes, including virulence and biofilm formation. When the compounds build to a threshold concentration and trigger gene expression, the signals are known as quorum-sensing (QS) signals. Examples of well-studied QS signals include acylhomoserine lactones, autoinducer 2, and peptide signals, but many other signals, such as indole, exist (3). In addition to signals, signal synthases, signal receptors, signal response regulators, and regulated genes (QS regulon) are key components of any QS system (4). For example, LuxI-type enzymes are signal synthases which synthesize acylhomoserine lactones. In addition, LuxR-type regulators are receptor proteins for the autoinducer signals, and signalreceptor binding is responsible for the expression of QS regulons. Since numerous compounds that inhibit QS have been identified, since QS is linked to virulence, and since inhibition of QS does not usually affect growth (in rich medium), it has been reasoned that inhibition of QS may be an effective means of reducing pathogenicity that is not subject to the usual resistance mechanisms of bacteria (1,(5)(6)(7)(8). Inhibition of QS is also known as quorum quenching (QQ) and is a form of antivirulence.The well-known examples (9) of QQ compounds include lactonases/acylases that degrade the N-(3-oxoctanoyl)-homoserine lactone (HSL) autoinducers, synthase inhibitors, like analogues of anthranilic acid that block synthesis of quinolone signals (10), and receptor inhibitors, such as brominated furanones (11). In addition, low concentrations of azithromycin, ceftazidime, and ciprofloxacin (antibiotics) inhibit QS in Pseudomonas aeruginosa (12). Also, among the thousands of drugs approved for clinical use, the anthelmintic drug niclosamide is a QQ compound (13); this drug reduces surface motility, biofilm formation, and production of the secreted virulence factors elastase, pyocyanin, and rhamnolipids. The rhizosphere bacterium Stenotrophomonas maltophilia produces cis-9-octadecenoic acid, which is a QQ compound that reduces violacein production by Chromoba...

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“…Some strategies have used chemical approaches such as antiadhesins/polymeric coatings (Cassinelli et al 2000;van de Belt et al 2001;von Bismarck et al 2001;John et al 2007;Johansson et al 2008), quorum sensing inhibitors (Francolini et al 2004;Cirioni et al 2006;Antoniani et al 2010), biosynthesis inhibitors (Ueda et al 2009), biofilm dispersal agents (Raad et al 2007;Kim et al 2008;Barraud et al 2009;Cai et al 2009;Davies & Marques 2009) and natural products (Bjarnsholt et al 2007;Kalishwaralal et al 2010;Kostenko et al 2010;Wu et al 2011), among others. Physical methods have also been investigated, including ultrasound waves (Ensing et al 2005), low frequency vibrations (Bandara et al 2014) and other methods (Di Poto et al 2009).…”

Section: Discussionmentioning

confidence: 97%

Magnetic fields suppressPseudomonas aeruginosabiofilms and enhance ciprofloxacin activity

et al. 2015

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Due to the refractory nature of pathogenic microbial biofilms, innovative biofilm eradication strategies are constantly being sought. Thus, this study addresses a novel approach to eradicate Pseudomonas aeruginosa biofilms. Magnetic nanoparticles (MNP), ciprofloxacin (Cipro), and magnetic fields were systematically evaluated in vitro for their relative anti-biofilm contributions. Twenty-four-hour biofilms exposed to aerosolized MNPs, Cipro, or a combination of both, were assessed in the presence or absence of magnetic fields (Static one-sided, Static switched, Oscillating, Static + oscillating) using changes in bacterial metabolism, biofilm biomass, and biofilm imaging. The biofilms exposed to magnetic fields alone exhibited significant metabolic and biomass reductions (p < 0.05). When biofilms were treated with a MNP/Cipro combination, the most significant metabolic and biomass reductions were observed when exposed to static switched magnetic fields (p < 0.05). The exposure of P. aeruginosa biofilms to a static switched magnetic field alone, or co-administration with MNP/Cipro/MNP + Cipro appears to be a promising approach to eradicate biofilms of this bacterium.

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Uracil influences quorum sensing and biofilm formation in Pseudomonas aeruginosa and fluorouracil is an antagonist

Cited by 142 publications

References 42 publications

Carbon Catabolite Control is Important for Listeria monocytogenes Biofilm Formation in Response to Nutrient Availability

Carbon Catabolite Control is Important for Listeria monocytogenes Biofilm Formation in Response to Nutrient Availability

Resistance to Quorum-Quenching Compounds

Magnetic fields suppressPseudomonas aeruginosabiofilms and enhance ciprofloxacin activity

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