Uracil—DNA glycosylase from Bacillus stearothermophilus

Heat‐labile uracil‐DNA glycosylase: purification and characterization

“…With respect to the -o J i ~ "'... monomeric character and the molecular weight the enzyme "~ is similar to the uracil-DNA glycosylases from other prokara: yotic organisms [3][4][5][6][7][8][9]. The molecular weight of 23 400 Da closely resembles those of the enzymes from B. subtilis and E. coli having a molecular weight of 24 000 and 24 500 Do, respectively [5,4].…”

“…From bacterial origins, LING has also been characterGermany). Hydroxyapatite was obtained from IBF Biotechnics (Vilized from Bacillus subtilis [5], B. stearothermophilus [6], Microleneuve-la-Garenne, France). The DIG-Taq DNA sequencing kit, DIG-luminescent detection kit, UNG from E. coli and all other recoccus luteus [7], Mycoplasma lactucae [8] and Thermothrix agents were from Boehringer Mannheim GmbH (Mannheim, Gerthiopara [9].…”

Cloning and Sequence Analysis of Novel DNA Polymerases from Thermophilic Geobacillus Species Isolated from Hot Springs in Turkey: Characterization of a DNA Polymerase I from Geobacillus kaue Strain NB

“…Cloning and Amino Acid Sequence Analysis of the polA Genes We designed degenerate primers (Table 1) in order to amplify the previously unknown polA genes from seven Geobacillus species based on the most conserved regions of previously known polA genes from the Bacillus species [8][9][10][11][12]. The full-length polA gene sequences were determined by plasmid DNA sequencing of the genes after their PCR-based cloning into pETM-20 expression vector (Novagen).…”

“…A few moderately thermostable DNA polymerase I have been purified and characterized from the thermophilic members of the family Bacillaceae [7], especially from the genus Bacillus and from a recently identified new genus Geobacillus: Bacillus stearothermophilus [8][9][10], Bacillus caldotenax [11,12], Geobacillus sp. MKK [13], and Geobacillus caldoxylolyticus TK4 [14].…”

The nuclear mutation Ocdts-1 changes the 2-D electrophoretic pattern of Drosophila mitochondria

“…inst., review by ZAKIM and VESSEY 1980). In other cases, however, the temperature induced change in activation energy does not follow fluidity changes of the bulk membrane lipids and in these cases changes in the lipids in the vicinity of membrane proteins (boundary lipids, JOST et al 1973;SHIMOMURA, and OZAWA 1981;CHAPMAN et al 1979) are thought to be responsible for changes in activation energy (BERTOLI et al 1976;BUTLER et al 1978).However, also many soluble enzymes show abrupt changes in Arrhenius activation energy (MAS-SEY et al 1966;ADAMS and SWART 1977;HOUGHTON 1979;KABOEV et al 1981). It is conceivable, therefore, that in some cases the breaks in Arrhenius plots of membrane bound enzymes are due to conformational changes intrinsic to the proteins (YAMAKI and UHITANI 1974; VAN DEN THILLART and MOUDERKOLK 1978;STANLEY and Luzio 1978;YOSHIDA et al 1979;BADOR et al 1984).Previously, it has been shown that the nuclear mutation Ocd".'…”