Uptake Studies on a Fluorescein-Labelled Seed Oil Adjuvant in Abutilon theophrasti, Sinapis arvensis and Beta vulgaris

Heini, Julia; Ayala, Victor Rueda; Walker, Frank; Mainx, Hans-Georg; Gerhards, Roland

doi:10.1007/s10343-012-0285-y

Cited by 2 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because the cuticle is a barrier to the uptake of pesticides by plants, better knowledge of compartment is essential for improving the transfer and delivery of such agrochemicals , and in the studies investigating their reactivity/photoreactivity inside the cuticle. , Fluorescence is a nondestructive technique that has been successfully employed to study the cuticle and the interactions with chemicals. For instance, fluorescence was used to visualize the distribution of fluorescent pollutants in leaves, , to monitor the uptake of labeled adjuvants or of xenobiotics through the cuticle, , or to image the cuticle architecture . In this study, a rapid and simple analytical method based on fluorescence was developed to evaluate the polarity of cuticles in situ while avoiding heavy sample manipulations and time-consuming analyses.…”

Section: Introductionmentioning

confidence: 99%

“…9,10 Fluorescence is a nondestructive technique that has been successfully employed to study the cuticle and the interactions with chemicals. For instance, fluorescence was used to visualize the distribution of fluorescent pollutants in leaves, 11,12 to monitor the uptake of labeled adjuvants or of xenobiotics through the cuticle, 13,14 or to image the cuticle architecture. 15 In this study, a rapid and simple analytical method based on fluorescence was developed to evaluate the polarity of cuticles in situ while avoiding heavy sample manipulations and time-consuming analyses.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Usefulness of Fluorescent Probe Prodan To Gain Insight into the Polarity of Plant Cuticles

Stawinoga

Sleiman

Chastain

et al. 2015

J. Agric. Food Chem.

View full text Add to dashboard Cite

Plant cuticle is a complex mixture of hydrophobic components that controls the uptake of pesticides by plants. Although the transport of lipophilic molecules across the cuticle has been intensively studied, development of tools to measure the cuticle polarity has received little attention. We developed a rapid and simple analytical method to evaluate the polarity of cuticles in situ. This method uses Prodan, 6-propionyl-2-(dimethylaminonaphthalene), a medium-sensitive fluorescent probe. Tests on model surfaces with varied polarity (i.e., wax paraffin, polyethylene, C18) were carried out to test the feasibility of the measurement and to optimize the application of Prodan. Moreover, on the basis of the Kamlet-Taft solvatochromic comparison method, a relationship between the emission characteristics of Prodan and the number of carbon atoms in primary alcohols mimicking the solid medium was established. After optimization, the method was validated on three natural plant cuticles (leaf of Zamiifolia, skin of green pepper, and skin of white grape).

show abstract