1986
DOI: 10.3109/00365518609083663
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Uptake of transcobalamin II-bound cobalamin by isolated rat kidney tubule cells

Abstract: The uptake and intracellular processing of transcobalamin II-bound cobalamin by isolated rat kidney tubule cells were studied. The cells absorbed the complex in a temperature-and calcium-dependent process, which could be inhibited by monensin, an inhibitor of endocytosis. Cells, loaded with a mixture of 125I- and 57Co-labelled transcobalamin II-vitamin B12, released 125I-labelled protein-degradation products, while keeping the 57Co-labelled vitamin. Protein degradation was inhibited by chloroquine and monensin… Show more

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Cited by 23 publications
(15 citation statements)
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“…The Ca2+-sensitive binding sites previously described (10,11) on renal proximal tubule cells may be identical to megalin, whereas TC-B12 binding sites on several nonepithelial cell types not expressing megalin must be of different origin. A protein with unknown primary structure has been purified by ligand-affinity chromatography (35,37,38).…”
Section: Discussionmentioning
confidence: 84%
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“…The Ca2+-sensitive binding sites previously described (10,11) on renal proximal tubule cells may be identical to megalin, whereas TC-B12 binding sites on several nonepithelial cell types not expressing megalin must be of different origin. A protein with unknown primary structure has been purified by ligand-affinity chromatography (35,37,38).…”
Section: Discussionmentioning
confidence: 84%
“…It is suggested to be a homodimer with an estimated size of 120-130 kDa (35) as judged by SDS/gel electrophoresis. The protein has also been detected by immunoblotting of isolated rat Mg2+-precipitated kidney basolateral membranes (40), and this (5,6,8,10,12) showing that the proportional uptake in the kidney of dietary B12 or intravenously administrated TC-B12 is higher at a high B12 load in the organism (5,6,8,12 were reported to inhibit the binding to this receptor, which might be identical to megalin. The binding of both the apo-and holo TC seems biological meaningful, because binding of only the holoform would result in loss of TC in the urine.…”
Section: Discussionmentioning
confidence: 97%
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