Uptake of Rubidium and Phosphate Ions by Polysaccharide-Producing Bacteria

Rorem, Edward S.

doi:10.1128/jb.70.6.691-701.1955

Cited by 19 publications

(5 citation statements)

References 28 publications

(22 reference statements)

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“…At the pH of the incubation solution (6.8), aspartate has a net negative charge, and specific adsorption could occur if the free energy of adsorption were negative. Adsorption of inorganic anions (46) and amino acid dipolar ions (26) by microbial extracellular material has been documented.…”

Section: Resultsmentioning

confidence: 99%

Multiplicity of aspartate transport in thin wastewater biofilms

Eighmy¹,

Bishop²

1984

Appl Environ Microbiol

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This research documents the multiplicity of L-aspartate transport in thin wastewater biofilms. A Lineweaver-Burk analysis of incorporation produced a curvilinear plot (concave down) that suggested active transport by two distinct systems (1 and 2). The inactivation of system 2 with AsO4 or osmotic shock resolved system 1, which was a high-affinity, low-capacity system with an apparent K, (Michaelis-Menten constant) of 4.3 ,uM (As04) or 4.6 ,iM (osmotic shock). The inactivation of system 1 with dinitrophenol resolved system 2, which was a low-affinity, high-capacity system with an apparent K, of 116.7 ,uM. System 1 was more specific for aspartate than system 2 in the presence of aspartate analogs. Sodium had no discernible effect on the incorporation velocities by either system. These results indicate that system 1 is a membrane-bound proton symport coupled to the proton gradient component of the proton motive force and that system 2 is a binding protein-mediated system coupled to phosphate bond energy. Analyses of diffusional limitations on the derived transport constants indicated that internal resistances were present but that the apparent constants were close to the intrinsic values, especially for system 1. Metabolic inactivation of the biofilm with dinitrophenol and AsO4 did not completely inactivate aspartate incorporation, which indicated that some simple adsorption of the aspartate anion by the biofilm had occurred. These results show that aspartate is transported by wastewater biofilm bacteria via systems with different affinities, specificities, and mechanisms of energy coupling.

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Section: Resultsmentioning

confidence: 99%

Multiplicity of aspartate transport in thin wastewater biofilms

Eighmy¹,

Bishop²

1984

Appl Environ Microbiol

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“…Attempts to isolate specific potassium-binding substances have so far been unsuccessful. Rorem (1955) examined the uptake of rubidium and phosphate by Leuconostoc mesenteroides, L. dextranicurn and Streptococcus salivaris. These bacteria can be caused to produce extracellular polysaccharide, or prevented from doing so by varying the form of carbohydrate in the medium.…”

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confidence: 99%

Salt Uptake in Plants

Sutcliffe

1959

Biological Reviews

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“…The work of Rorem (1955) provides an alternative basis for estimating the ability of bacteria to absorb rubidium. He showed that different species of bacteria could absorb from 15 to 400 m-equiv.…”

Section: Resultsmentioning

confidence: 99%

The Absorption of Ions by Microorganisms and Excised Roots

Barber

1974

New Phytologist

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SUMMARYMicro-organisms are present in sufficient numbers on barley roots to account for the differences previously attributed to them in experiments comparing the uptake of rubidium by sterile and non-sterile excised roots. Experiments with thallium, which is highly phytotoxic at concentrations above 0.2 mM, provide further grounds for rejecting the postulate, based on 'dual absorption isotherms', that under physiological conditions different mechanisms of absorption operate depending on the external concentration.

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Uptake of Rubidium and Phosphate Ions by Polysaccharide-Producing Bacteria

Cited by 19 publications

References 28 publications

Multiplicity of aspartate transport in thin wastewater biofilms

Multiplicity of aspartate transport in thin wastewater biofilms

Salt Uptake in Plants

The Absorption of Ions by Microorganisms and Excised Roots

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