Uptake of oleoyl-chitosan nanoparticles by A549 cells

Zhang, Jing; Chen, Xi Guang; Wei, Peng; Liu, Chengsheng

doi:10.1016/j.nano.2008.03.006

Cited by 125 publications

(128 citation statements)

References 28 publications

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“…On the basis of these experiments, we conclude that the NPs deliver calcein to the cytosol of cells by coendocytosis of this dye and NPs, followed by NP-induced disruption of endosomes and escape of the dye into the cytosol. The cellular uptake of CS-NPs seems to occur by adsorptive endocytosis, as the uptake of similar NPs was previously reported to occur predominantly by adsorptive endocytosis in A549 cells [49] as well as in the HeLa cell line [15].…”

Section: Discussionmentioning

confidence: 57%

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

et al. 2013

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Nanoparticles with pH-sensitive behavior may enhance the success of chemotherapy in many cancers by efficient intracellular drug delivery. Here, we investigated the effect of a bioactive surfactant with pH-sensitive properties on the antitumor activity and intracellular behavior of methotrexate-loaded chitosan nanoparticles (MTX-CS-NPs). NPs were prepared using a modified ionotropic complexation process, in which was included the surfactant derived from N α ,N ε -dioctanoyl lysine with an inorganic lithium counterion. The pH-sensitive behavior of NPs allowed accelerated release of MTX in an acidic medium, as well as membrane-lytic pH-dependent activity, which facilitated the cytosolic delivery of endocytosed materials. Moreover, our results clearly proved that MTX-CS-NPs were more active against the tumor HeLa and MCF-7 cell lines than the free drug. The feasibilty of using NPs to target acidic tumor extracellular pH was also shown, as cytotoxicity against cancer cells was greater in a mildly acidic environment. Finally, the combined physicochemical and pH-sensitive properties of NPs generally allowed the entrapped drug to induce greater cell cycle arrest and apoptotic effects. Therefore, our overall results suggest that pH-sensitive MTX-CS-NPs could be potentially useful as a carrier system for tumor and intracellular drug delivery in cancer therapy.

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Section: Discussionmentioning

confidence: 57%

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

et al. 2013

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“…1,9,26,[40][41][42][43][44] Figure 1 shows a comparison of the kinetics of uptake of silica nanoparticles in A549 cells in serum free and complete media, obtained by flow cytometry, as described in the Methods. As anticipated in Supplementary Figure S1, the kinetic study also clearly indicated that uptake was always higher when the nanoparticles were exposed to cells in SF.…”

Section: Resultsmentioning

confidence: 99%

Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

et al. 2012

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“…Therefore, the size of nanoparticles used in our study is within the optimal range. 29 The ability of FITC-labeled BSANPs with or without RGD peptide conjugation to target avβ3 positive BxPC-3 cells was evaluated by fluorescence microscopy. The results clearly indicated that RGD-conjugated BSANPs selectively accumulate in BxPC-3 cells when compared with BSANPs without RGD conjugation.…”

Section: Discussionmentioning

confidence: 99%

“…It was noted that, the uptake level reached the maximum and then declined after 24 h. The decrease of fluorescencent intensity in nanoparticle uptake could be attributed to the degradation or photobleaching of FITC. 29,32 In addition, RGDcontaining peptides were found to be able to directly induce cell apoptosis, which could also cause the reduction of fluorescencent intensity.…”

Section: Discussionmentioning

confidence: 99%

“…The synthesis of FITC-labeled nanoparticles was based on the reaction between the isothiocyanate group of FITC and the primary amino group of nanoparticles. 28,29 Briefly, FITC was mixed with BSANPs in sodium bicarbonate buffer, pH 9.5, followed by incubation for 20-24 h in darkness at room temperature while stirring. It was then dialyzed against distilled water using a cellulose membrane (molecular weight cutoff 8,000-12,000) for 2 d and lyophilized.…”

Section: Methodsmentioning

confidence: 99%

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RGD-conjugated albumin nanoparticles as a novel delivery vehicle in pancreatic cancer therapy

Zhang

et al. 2012

Cancer Biology & Therapy

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These authors contributed equally to this work. Keywords: nanoparticles, albumin, RGD, FITC, delivery, pancreatic cancer, therapyIntegrin avb3 receptor is expressed on several types of cancer cells, including pancreatic cancer cells, and plays an important role in tumor growth and metastasis. The ability to target the integrin avb3 receptor on cancer cells increases the efficacy of targeted therapy and reduces the side effects. The aim of this study is to develop a novel arginine-glycineaspartic acid (RGD) peptide-conjugated albumin nanoparticle to enhance the intracellular uptake of anticancer drug into the pancreatic cancer cells through receptor-mediated endocytosis. In cellular uptake studies, the fluorescent signal of RGD-conjugated BSANPs in BxPC3 cells was higher than that of BSANPs without RGD conjugation as determined by fluorescence spectrophotometer. We also found that BSANPs bound to BxPC3 cells in a time-and concentration-dependent manner. The uptake of RGD-conjugated BSANPs by pancreatic cancer cells was inhibited by an excess amount of free RGD peptide, indicating that the binding and/or uptake were mediated by the avb3 receptor. Furthermore, the nanoparticles were found to be located close to the nuclei by using laser scanning confocal microscopy. Besides, no significant in vitro cytotoxicity was observed as measured with MTT assay. Both in vitro and in vivo antitumor efficacy was improved by targeting gemcitabine-loaded nanoparticles to BxPC-3 cells using RGD peptides. Therefore, the RGD-conjugated BSANPs hold great potential as an effective drug delivery system to deliver therapeutic agents to pancreatic cancer.

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Uptake of oleoyl-chitosan nanoparticles by A549 cells

Cited by 125 publications

References 28 publications

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles

Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

RGD-conjugated albumin nanoparticles as a novel delivery vehicle in pancreatic cancer therapy

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