Wood y ash from biofuel combustion plants is land lled commonly as a waste, but research of this material is needed because it may reduce future fertilizer requirements. We investigated the e ect of the ash application on potential changes in pH, nutrient, and potentially toxic element (in our case As, Cd, and Pb) content and mobility in soils. Wood y ash in doses up to 1% (w/w) was added to spring wheat (Triticum aestivum L.) grown in potentially toxic element-contaminated loam (Cambisol) and sandy clay loam (Fluvisol) soil material in 3-yr pot experiment. e potentially toxic element contents in soils exceeded the maximum permissible limits for Czech soils, but the plant availability was, thanks to ash addition, limited. e e ect of wood ash was greater in the Cambisol where Cd, Zn, and also Pb showed similar trends, and their content in plant decreased (Cd by 60%, Zn by 50%, and Pb by 45%), whereas the nutrient contents tended to increase in plants. Di erences were usually insigni cant (α = 0.05) in the Fluvisol between treatments, and Ca, Mg, and P contents were highest in the treatment without ash addition. e in uence of soil changes on element uptake and distribution within the aboveground plant biomass was also observed. ese ndings are an important step on providing evidence of the bene ts of using wood y ash as a fertilizer supplement, because potentially toxic element contents in wheat grown in contaminated soils with ash addition were low and decreased, whereas the concentrations of major nutrients increased.