Uptake of lead by human erythrocytes <i>in vitro</i>

Clarkson, T. W.; Kench, J. E.

doi:10.1042/bj0690432

Cited by 81 publications

(29 citation statements)

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“…Other factors, apart from the pump and leak, may also be involved. Cells and plasma contain miIlimo~ar concentrations of phosphate, so lead phosphate sols may be present [2], and some Pb may be bound to plasma proteins.…”

Section: Discussionmentioning

confidence: 99%

Active transport of lead by human red blood cells

Simons

1984

FEBS Letters

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Human red cells suspended in lead‐citrate buffers (2.6μM Pb2+) take up much less Pb than predicted from studies of equilibrium binding of Pb to haemolysates. Pb uptake is increased by ATP depletion, or by loading at 0°C. Tracer studies with 203Pb indicate that the low uptake at 37°C in the presence of substrate is not due to membrane impermeability to Pb. Cold‐loaded cells extrude Pb against a concentration gradient at 37°C when glucose is present. These results suggest that the cellular loading of Pb is dependent on the balance between an inward leak and an outward pump. The extrusion of Pb from the cells is possibly brought by the Ca pump.

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Section: Discussionmentioning

confidence: 99%

Active transport of lead by human red blood cells

Simons

1984

FEBS Letters

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“…The "normal range" found was about 5-40 ,ug/100 ml,t but the values obtained included 45,50,53,60, and 100 (New York City) ,ug/100 ml with, in all likelihood, more values in the latter range. The distribution was skewed as seen by the low median (18 ,g/100 ml) and mean (17 ,ug/100 ml).…”

Section: August 1977mentioning

confidence: 99%

“…In in vitro tests, lead is transferred rather quickly from the plasma to erythrocytes (45,46). At low PbB values and under some other conditions, as noted below, the plasma and serum contain a higher percentage or an additional amount of lead.…”

Section: Erythrocytes Soft Tissues and Bonesmentioning

confidence: 99%

“…" Chisolm (15) found that FEP is a sensitive indicator of the "internal dose" of lead as long as iron deficiency and its concomitant anemia were mathematically corrected by a determination of Hct or Hb. "In children with hematocrit :36% and PbB <30 ,ug/100 ml, we have found that erythrocyte protoporphyrin levels are low and independent of PbB concentration ... ¢90% of children with PbB ¢50 ,tg/100 ml are positive reactors and that 50% response rate is associated with PbB in the [40][41][42][43][44][45][46][47][48][49][50] ,ug/100 ml range (15)." Elsewhere (89) it is stated that "significant numbers of children with blood lead levels of [30][31][32][33][34][35][36][37][38][39] ,ug/100 ml have shown evidence of metabolic impairment as detected by EP test-ing.…”

Section: Ppe (Fep) Erythrocyte Protoporphyrin and Freementioning

confidence: 99%

“…For Na+K+ATPase activity < 40 ,umole PJ/hr/mg tyrosine, [20][21][22][23][24][25][26][27][28][29] ,tg Pb/100 ml; for GSH < 58 ,g/100 ml of erythrocytes, 25-30 ,ug/100 ml. Anemia (decreased Hb) does not occur in otherwise healthy adults below 70-80 ixg/100 ml, and in socioeconomically poor children below [40][41][42][43][44][45][46][47][48][49][50] ,g/100 ml.…”

Section: Ppe (Fep) Erythrocyte Protoporphyrin and Freementioning

confidence: 99%

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Indices of potential lead hazard.

Posner¹

1977

Environ Health Perspect

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This review is concerned with the concentrations of lead in human whole blood, erythrocytes, plasma, serum, soft tissues, bone, and urine. The extent to which redistribution of some of the bound lead occurs is outlined. The effects of lead on enzyme activities and on the accumulation of metabolic intermediates in the blood and urine are described. A brief section deals with the range ofsigns and symptoms that can occur and differences seen between symptomatic children and adults.

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Organ retention and excretion of radiolead administered intravenously in rats: A Comparison of erythrocyte- and plasma-associated lead

Barton¹,

Conrad

2001

J. Trace Elem. Exp. Med.

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Organ retention and excretion of lead transported by erythrocytes may differ from plasma. To evaluate this, we administered inorganic radiolabeled lead to adult male rats intravenously after its incorporation into erythrocytes or incubation with plasma. The percentage of remaining whole‐body radiolead lead retained in major organs was similar in animals dosed with erythrocyte‐ and plasma‐associated radiolead, but less erythrocyte‐associated radiolead was excreted in urine and stool. Calcium disodium EDTA significantly decreased the whole‐body retention of plasma‐associated radiolead in comparison with erythrocyte‐associated radiolead; dimercaprol and penicillamine did not affect whole‐body radiolead retention. However, the percentages of erythrocyte‐ and plasma‐associated radiolead in major organs were similar in rats that received calcium disodium EDTA, dimercaprol, or penicillamine and in normal controls. The whole‐body retention of radiolead was similar in rats with acetylphenylhydrazine (APH)‐induced anemia and in normal control rats. However, the percentages of radiolead retained in the livers and spleens of APH‐treated rats were significantly greater. We conclude that the percentages of erythrocyte‐ and plasma‐associated lead retained in the major organs of normal rats are similar. However, erythrocyte‐associated lead may be deposited preferentially in the liver and spleen when hemolytic anemia is present. Erythrocyte‐associated lead is excreted less readily than lead in plasma. J. Trace Elem. Exp. Med. 14:89–98, 2001. © 2001 Wiley‐Liss, Inc.

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Uptake of lead by human erythrocytes in vitro

Cited by 81 publications

References 4 publications

Active transport of lead by human red blood cells

Active transport of lead by human red blood cells

Indices of potential lead hazard.

Organ retention and excretion of radiolead administered intravenously in rats: A Comparison of erythrocyte- and plasma-associated lead

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