“…The uptake of 18 F-galacto-RGD and 3 H-DG to the vessel wall was studied by digital autoradiography of aortic tissue sections as previously described 20,21 2 ) with an image analysis program (Tina 2.1, Raytest Isotopenmessgeräte, GmbH, Straubenhardt, Germany). After careful coregistration of autoradiography and hematoxylin and eosin-stained histological images, 3 types of regions of interest (ROI) were defined according to morphology in 3 to 7 sections from each mouse: (1) normal vessel wall (no lesion formation, nϭ74), (2) plaque (excluding media, nϭ150), and (3) adventitia (nϭ99).…”