Uptake of Dinitrophenol &amp; its Effect on Transpiration &amp; Calcium Accumulation in Barley Seedlings

Barber, D. A.; Koontz, H. V.

doi:10.1104/pp.38.1.60

Cited by 36 publications

(10 citation statements)

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“…Although some studies have indicated a constant rate of absorption of Ca (9,14,19,25) and Sr (8, 9) for periods of many hours, others have disclosed that after an initial rapid exchange-adsorption, further uptake of Ca (28) and Mn (32) is very slow and appears to be nonmetabolic, in that a notable insensitivity to low temperatures (28,34) and metabolic inhibitors (1,28,32) is displayed.…”

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confidence: 99%

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Effect of Metabolic Inhibitors and Temperature on Uptake and Translocation of ⁴⁵Ca and ⁴²K by Intact Bean Plants

Drew

Biddulph

1971

Plant Physiol.

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The dependence of Ca uptake and translocation by intact roots of Phaseolus vulgaris on concurrent root metabolism was investigated using 'Ca-labeled Hoagland solutions at one-half and one-twentieth strength (2.5 and 0.25 mM Ca'). Adsorbed and absorbed "5Ca fractions in the roots were distinguished on the basis of the time course of exchange with the outer solution. Uptake of '2K, of which the characteristics are better known, was measured for comparison. The absorbed 'Ca fraction showed a markedly nonlinear increase with time in contrast to the near linear increase in 'K. Exposure of roots to cyanide, arsenate, 2,4-dinitrophenol, or low temperatures caused only slight reductions in '5Ca absorption by roots, but significant reductions of 'K. In all treatments involving inhibitors and low temperatures, the translocation to shoots of both 'Ca and 'K was strongly inhibited. The conclusion that much of the absorbed "Ca fraction in the root tissue is taken up by processes which are not rate-limited by metabolism is discussed.The convention of dividing cations bound by roots into two fractions, adsorbed and absorbed, on the basis of the time course of uptake and exchange has proved convenient for the monovalent cations (4,9,24). The adsorbed fraction characteristically reaches equilibrium with the bathing solution within minutes and is independent of metabolic processes. This process is considered to represent a simple nonspecific ion exchange within cell wall surfaces. The absorbed fraction is characterized by a slower rate of increase which continues for many hours. It is not readily exchangeable with the outer solution and is dependent on concurrent metabolism. The absorbed fraction may represent a net increase in ion content of the tissue or, for roots which have reached dynamic equilibrium with the outer solution, may represent tracer influx. It is implicit that the absorbed ions are retained within protoplasts, although precise localization of the two fractions has rarely been attempted.The characterization of uptake and retention of Ca and other divalent cations has shown less uniformity than that for the monovalent cations. Although some studies have indicated a constant rate of absorption of Ca (9,14,19,25) Measurements of uptake of Ca and other divalent cations by plant tissues is often complicated by the relatively large fraction held on adsorption-exchange sites in cell walls (5,8,9,36). In experiments with labeled ions the usual procedure for displaying this has been to immerse the root tissue at the end of a 'Ca uptake period in an excess of unlabeled solution for 30 min to remove the fraction designated as adsorbed; the amount retained by the root has been considered metabolically absorbed by the root cells (8, 9). For the sake of comparing the present results with previous studies the above terminology and methods have been retained.In this paper we present observations on the uptake of 45Ca by intact bean plants under conditions similar to those used to display, by microautoradiography, the lo...

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“…1 Concentration factor = ,umoles translocated to shoot -(ml transpired X external concentration, Mmoles/ml). 2 Significant differences between low and high transpiration rates, P < 0.001.…”

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Effect of Metabolic Inhibitors and Temperature on Uptake and Translocation of ⁴⁵Ca and ⁴²K by Intact Bean Plants

Drew

Biddulph

1971

Plant Physiol.

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“…This view is supported byobservations on tomatoes (Jackson and Weatherly 1962) and on barley for transport of calcium (Barber and Koontz 1963), and by the following examples.…”

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Sodium and Potassium Uptake by Seedlings of Hordeum Vulgare

Pitman¹

1965

Aust. Jnl. Of Bio. Sci.

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Uptake of potassium and sodium from culture solutions by barley seedlings under a range of experimental conditions has been determined. It was shown that both sodium and potassium transport to the shoot was due to an active process in the root which was limited by the plant's growth. This process was not available for uptake of divalent cations. Sodium uptake at higher concentrations showed some relation to transpiration and the resulting increase in sodium uptake with concentration reduced the selectivity in the plant for potassium to sodium. It was shown that potassium transport across the root was independent of water flow to the cytoplasm. The role of the cytoplasm in selective ion uptake to the shoot is discussed.

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“…1973, it has been hypothesized that Ca may be unable to enter vascular tissues in mature zones of roots due to the suberization of endodermal cell walls (Shone et al 1973). Since intact plants of most species are known lo take up substantial amounts of Ca, these observations appear to support the concept of Ca uptake and transport by bulk flow of solution (MooTt et al 1961, Barber andKoontz 1963).…”

Section: Introductionmentioning

confidence: 96%

“…Moore e( al. 1961, Barber andKoontz 1963). Because the absorption and upward transport of Ca decreases very rapidly with the maturity of root tissues (Wiebe and Kramer 1954, Canning and Kramer 1958, Clarkson et al 1971.…”

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confidence: 99%

The Accumulation and Transport of Calcium in Barley Roots

Singh

Jacobson

1979

Physiologia Plantarum

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The accumulation and transport of Ca by various zones of 6‐day old barley roots (Hordeum vulgare L.) were examined with special reference to their relationship to the salt status of the root. The initial salt content had a profound effect on Ca transport and a lesser effect on Ca accumulation. High‐salt roots transported Ca in much larger amounts than did low salt roots. In low salt roots the apical zone was more active in transporting Ca to the conducting tissue than were the mid or basal zones However, in high salt roots all zones were about equally active in transporting Ca. The metabolic inhibitor, DNP, had little effect on accumulation but inhibited transport very effectively. The effect of DNP was more pronounced on transport from the apical zone than from the other root zones. Calcium applied anywhere along the root length moved only basally and its polarized longitudinal movement was maintained irrespective of the salt status of the root. The movement of Ca was characterized by a rapid release of preabsorbed Ca and a ready exchange of apoplastic Ca. The hypothesis is presented that cellular Ca is in a relatively mobile state. Its entry into the symplasm is the rate limiting step in longitudinal transport and its overall movement is metabolically controlled.

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Uptake of Dinitrophenol & its Effect on Transpiration & Calcium Accumulation in Barley Seedlings

Cited by 36 publications

References 11 publications

Effect of Metabolic Inhibitors and Temperature on Uptake and Translocation of ⁴⁵Ca and ⁴²K by Intact Bean Plants

Effect of Metabolic Inhibitors and Temperature on Uptake and Translocation of ⁴⁵Ca and ⁴²K by Intact Bean Plants

Sodium and Potassium Uptake by Seedlings of Hordeum Vulgare

The Accumulation and Transport of Calcium in Barley Roots

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Uptake of Dinitrophenol & its Effect on Transpiration & Calcium Accumulation in Barley Seedlings

Cited by 36 publications

References 11 publications

Effect of Metabolic Inhibitors and Temperature on Uptake and Translocation of 45Ca and 42K by Intact Bean Plants

Effect of Metabolic Inhibitors and Temperature on Uptake and Translocation of 45Ca and 42K by Intact Bean Plants

Sodium and Potassium Uptake by Seedlings of Hordeum Vulgare

The Accumulation and Transport of Calcium in Barley Roots

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Effect of Metabolic Inhibitors and Temperature on Uptake and Translocation of ⁴⁵Ca and ⁴²K by Intact Bean Plants

Effect of Metabolic Inhibitors and Temperature on Uptake and Translocation of ⁴⁵Ca and ⁴²K by Intact Bean Plants