Uptake of cell‐penetrating peptides in yeasts

Holm, Tina; Netzereab, Semharai; Hansen, Mats; Langel, Ülo; Hällbrink, Mattias

doi:10.1016/j.febslet.2005.07.099

Cited by 45 publications

(39 citation statements)

References 12 publications

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“…Nevertheless, for individual CPPs structural modifications that improve metabolic stability are expected to contribute to enhanced translocation. Holm et al119 reported about uptake and metabolic stability of CPPs in two yeast species, Saccharomyces cervisiae and Candida albicans . The intracellular degradation of the three investigated CPPs, namely pVEC, penetratin and (KFF) 3 K, varied from complete stability to complete degradation.…”

Section: Cellular Barriers Limiting Cpp Translocationmentioning

confidence: 99%

“…Very recently, the uptake of three CPPs, namely pVEC, penetratin and (KFF) 3 K, into two yeast species, Saccharomyces cervisiae and Candida albicans was studied by Holm et al119 By comparing the capacity of the investigated CPPs to traverse the yeast cell envelope the authors could show that the cellular uptake of the peptides varied widely. pVec and (KFF) 3 K displayed the highest translocation into yeast, although being partly degraded.…”

Section: New Perspectives For Application Of Cpps In Drug Deliverymentioning

confidence: 99%

“…pVec and (KFF) 3 K displayed the highest translocation into yeast, although being partly degraded. Considering the stability of pVEC in both yeast species, it was suggested as attractive candidate for further studies 119. Nevertheless, to date the therapeutic value of CPP based antimicrobials has yet to be established.…”

Section: New Perspectives For Application Of Cpps In Drug Deliverymentioning

confidence: 99%

See 2 more Smart Citations

On The Biomedical Promise of Cell Penetrating Peptides: Limits Versus Prospects

Foerg

Merkle

2008

Journal of Pharmaceutical Sciences

125

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Section: Cellular Barriers Limiting Cpp Translocationmentioning

confidence: 99%

Section: New Perspectives For Application Of Cpps In Drug Deliverymentioning

confidence: 99%

Section: New Perspectives For Application Of Cpps In Drug Deliverymentioning

confidence: 99%

See 1 more Smart Citation

On The Biomedical Promise of Cell Penetrating Peptides: Limits Versus Prospects

Foerg

Merkle

2008

Journal of Pharmaceutical Sciences

125

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“…It is clear that different cell lines have different membrane characteristics, which influence the cell-penetrating properties [17], [22], [25], [27], [30], [32]–[34], [37], [38], [40], [41], [43]–[45], [53], [55], [56], [58], [64]–[67]. We also assumed penetratin as a general positive control, because it is quite often used and is well characterized, being one of the first described CPPs.…”

Section: Discussionmentioning

confidence: 99%

Chemical-Functional Diversity in Cell-Penetrating Peptides

et al. 2013

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Cell-penetrating peptides (CPPs) are a promising tool to overcome cell membrane barriers. They have already been successfully applied as carriers for several problematic cargoes, like e.g. plasmid DNA and (si)RNA, opening doors for new therapeutics. Although several hundreds of CPPs are already described in the literature, only a few commercial applications of CPPs are currently available. Cellular uptake studies of these peptides suffer from inconsistencies in used techniques and other experimental conditions, leading to uncertainties about their uptake mechanisms and structural properties. To clarify the structural characteristics influencing the cell-penetrating properties of peptides, the chemical-functional space of peptides, already investigated for cellular uptake, was explored. For 186 peptides, a new cell-penetrating (CP)-response was proposed, based upon the scattered quantitative results for cellular influx available in the literature. Principal component analysis (PCA) and a quantitative structure-property relationship study (QSPR), using chemo-molecular descriptors and our newly defined CP-response, learned that besides typical well-known properties of CPPs, i.e. positive charge and amphipathicity, the shape, structure complexity and the 3D-pattern of constituting atoms influence the cellular uptake capacity of peptides.

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“…They cause membrane alterations such as stable or transient destabilization accompanied by folding of the peptide on the lipid membrane (60)(61)(62).…”

Section: Translocation Mechanismmentioning

confidence: 99%

Cell‐penetrating Peptides as a Novel Transdermal Drug Delivery System

et al. 2012

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In the last decade, almost one-third of the newly discovered drugs approved by the US FDA were biomolecules and biologics. Effective delivery of therapeutic biomolecules to their target is a challenging issue. Innovations in drug delivery systems have improved the efficiency of many of new biopharmaceuticals. Designing of novel transdermal delivery systems has been one of the most important pharmaceutical innovations, which offers a number of advantages. The cell-penetrating peptides have been increasingly used to mediate delivery of bimolecular cargoes such as small molecules, small interfering RNA nucleotides, drug-loaded nanoparticles, proteins, and peptides, both in vitro and in vivo, without using any receptors and without causing any significant membrane damage. Among several different drug delivery routes, application of cell-penetrating peptides in the topical and transdermal delivery systems has recently garnered tremendous attention in both cosmeceutical and pharmaceutical research and industries. In this review, we discuss history of cell-penetrating peptides, cell-penetrating peptide ⁄ cargo complex formation, and their mechanisms of cell and skin transduction.

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Uptake of cell‐penetrating peptides in yeasts

Cited by 45 publications

References 12 publications

On The Biomedical Promise of Cell Penetrating Peptides: Limits Versus Prospects

On The Biomedical Promise of Cell Penetrating Peptides: Limits Versus Prospects

Chemical-Functional Diversity in Cell-Penetrating Peptides

Cell‐penetrating Peptides as a Novel Transdermal Drug Delivery System

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