Uptake of Ca2+ by isolated secretory vesicles from adrenal medulla

Ca2+ uptake to purified secretory vesicles from bovine neurohypophyses

“…The Ca 2 + influx and efflux observed with neurohypophysial secretory vesicles in its properties is very similar to the process found in intact secretory vesicles from adrenal medulla [28,29]. The process described so far with intact secretory vesicles from neurohypophysis and adrenal medulla very much resembles the Ca2+ for Na + exchange that takes place of over the cell membrane of nerve and muscle cells [2].…”

Calcium dynamics in catecholamine-containing secretory vesicles

“…Regarding alternative Ca 2+ transport systems in the granules, the presence of either Ca 2+ -ATPases, Na + /Ca 2+ or H + /Ca 2+ exchange systems have been proposed in different preparations [14][15][16][17][18]. Inhibition of sarcoendoplasmic reticulum Ca 2+ -ATPase with 10 M benzohydroquinone produced little effects on [Ca 2+ ] SG (data not shown), suggesting that this type of Ca 2+ -ATPase was not involved.…”

Distribution of chromaffin secretory vesicles, acetylcholinesterase, and lysosomal enzymes in sucrose and percoll gradients

“…Protein, acety1cholinesterase, and absorbance at 280 nm were determined as described recently (7,13). The details of the methods used in the assays for five different acid hydrolases (14)(15)(16) are given in Table 1.…”