Uptake of [3H]-fosfomycin into cells of enterohemorrhagic Escherichia coli O157 and E. coli non-O157

Tsuboi, Isami; Ida, Hirohisa; Yoshikawa, Eiji; Hiyoshi, Suehiro; Yamaji, Emiko; Nakayama, Issei; Ohara, Koji; Nonomiya, Tomoko; Shigenobu, Fritz; Taniguchi, Kazuo; Shimizu, Masaki; Sawai, Tetsuo; Mizuoka, K

doi:10.1016/s0009-8981(98)00168-5

Cited by 7 publications

(6 citation statements)

References 8 publications

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“…Additionally, we evaluated the susceptibilities of STEC isolates to various oral antibiotics. In previous studies, the emergence of STEC strains resistant to ampicillin, gentamicin, tetracycline, streptomycin, sulfisoxazole, and/or trimethoprimsulfamethoxazole has been reported (4,9,21). Our results showed the presence of STEC isolates for which MICs of fosfomycin, ampicillin, cefaclor, kanamycin, tetracycline, and/or doxycycline were high, whereas all STEC isolates were susceptible to gentamicin, minocycline, norfloxacin, and azithromycin.…”

Section: Discussionsupporting

confidence: 60%

Emergence of Fosfomycin-Resistant Isolates of Shiga-Like Toxin-Producing Escherichia coli O26

Horii

Kimura

Sato

et al. 1999

Antimicrob Agents Chemother

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We evaluated the susceptibilities of 129 Shiga-like toxin-producingEscherichia coli (STEC) isolates to various antibiotics. The numbers of isolates for which MICs were high (≧128 μg/ml) were as follows: 5 for fosfomycin, 14 for ampicillin, 1 for cefaclor, 6 for kanamycin, 22 for tetracycline, and 2 for doxycycline. For two isolates of STEC O26 MICs of fosfomycin were high (1,024 and 512 μg/ml, respectively). Conjugation experiments and glutathioneS-transferase assays suggested that the fosfomycin resistance in these isolates was determined not by a plasmid but chromosomally. The amount of active intracellular fosfomycin in these STEC isolates was 100- to 200-fold less than that in E. coli C600 harboring pREFTT47B408 in the presence of eitherl-α-glycerophosphate or glucose-6-phosphate. Cloning, sequencing, and Northern blot analysis demonstrated that the transcriptional level of the murA gene encoding UDP-N-acetylglucosamine enolpyruvoyl transferase in these isolates was greater than that in E. coli C600. Our results suggest that the fosfomycin resistance in these STEC isolates is due to concurrent effects of alteration of the glpT and/oruhp transport systems and of the enhanced transcription of the murA gene.

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Section: Discussionsupporting

confidence: 60%

Emergence of Fosfomycin-Resistant Isolates of Shiga-Like Toxin-Producing Escherichia coli O26

Horii

Kimura

Sato

et al. 1999

Antimicrob Agents Chemother

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“…Furthermore, it is known that phosphorus moieties regulate important biological functions by mimicking carboxylic acid groups . In particular, fosfomycin ( 3 , X = O) is a broad spectrum antibiotic and is used in vitro and clinically (Figure ) . 1-Alkoxycarbonyl-2-phosphonoaziridine ( 4 ) also shows antibacterial activity .…”

Section: Three Membered Ringsazirines and Aziridinesmentioning

confidence: 99%

“…2 In particular, fosfomycin (3, X ) O) is a broad spectrum antibiotic 3 and is used in vitro 4 and clinically (Figure 2). 5 1-Alkoxycarbonyl-2phosphonoaziridine (4) also shows antibacterial activity. 6 For these reasons, functionalized azirines and aziridines containing a phosphonate group at the C-2position are expected to play a similar role to that observed in their isosteric analogues.…”

Section: Introductionmentioning

confidence: 99%

Synthetic Methods for Azaheterocyclic Phosphonates and Their Biological Activity

2004

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“…12) We previously reported that the minimum bactericidal concentrations (MBC) in 6 strains of E. coli O157 are high for fosfomycin, ampicillin, and tetracycline but low for ciprofloxacin, polymyxin B, cefoperazone, and kanamycin. 13) However, in these studies, drug susceptibility of other enterohaemorrhagic E. coli than E. coli O157 was not examined.…”

Section: Discussionmentioning

confidence: 99%

“…However, there are no data on drug susceptibility of enterohaemorrhagic E. coli other than E. coli O157. [11][12][13] Therefore, we evaluated E. coli O26, O111, and O165 which could be acquired in addition to E. coli O157.…”

Section: )mentioning

confidence: 99%

In Vitro Susceptibility of Four Serotypes of Enterohaemorrhagic Escherichia coli to Antimicrobial Agents.

Oie

Kawakami

Kamiya

et al. 2002

Biological & Pharmaceutical Bulletin

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Escherichia coli (E. coli) serotype O157 : H7 was first recognized as a human pathogen during a 1982 investigation of two outbreaks of diarrhea, and is estimated to cause more than 20000 cases of infection and as many as 250 deaths each year in the United States.1,2) In Japan, the incidence of this infection is also high as was observed in a large outbreak stemming from a school lunch in Sakai City in 1996 in which 12680 people were infected with E. coli O157 : H7, and 3 of them died. 3)There is continuing controversy as to whether antimicrobial therapy should be performed for E. coli O157 : H7 infection. Some investigators have suggested that administration of antimicrobial agents for this infection promotes release of verotoxin from this serotype or tends to induce secondary infection, thus arguing against antimicrobial therapy.4,5) Others have reported no effects of administration of antimicrobial agents for E. coli O157 : H7 infection on the development of hemolytic uremic syndrome (HUS) or the microorganism discharge period. 6,7) In contrast, some studies have shown a significant decrease in the incidence of HUS after administration of appropriate antimicrobial agents such as fluoroquinolones for E. coli O157 : H7 infection, or a significant decrease in the mortality rate after administration of a fluoroquinolone to this infection in a mouse model. [8][9][10] In general, effective oral antimicrobial agents should be administered for bacterial enterocolitis. 9) Enterohaemorrhagic E. coli has many 0 serotypes (1,2,4,5,6,18, 25, 26, 38, 39, 45, 50, 55, 82, 84, 91, 103, 111, 113, 114, 115, 117, 118, 121, 128, 145, 146, 153, 163, 165) in addition to O157. However, there are no data on drug susceptibility of enterohaemorrhagic E. coli other than E. coli O157.11-13) Therefore, we evaluated E. coli O26, O111, and O165 which could be acquired in addition to E. coli O157. MATERIALS AND METHODS Susceptibility TestsThe minimum inhibitory concentrations (MIC) were determined for the 83 strains of enterohaemorrhagic E. coli after 18 h incubation at 35°C by dilution on Mueller-Hinton agar (Difco, U.S.A.) under both aerobic and anaerobic conditions. As a control, E. coli NIHJ JC-2 was used. The following agents were tested: ciprofloxacin (Bayer Yakuhin, Ltd.), norfloxacin (Kyorin Pharm.), fosfomycin Na (Meiji Seika Kaisha, Ltd.) with or without 25 mg/ml of glucose-6-phosphate (G-6-P; Oriental Yeast Co., Ltd.) added, 14) kanamycin sulfate (Meiji Seika Kaisha, Ltd.), cefoperazone Na (Pfizer Pharm, Inc.), and ceftazidime (Glaxo Japan Co.). These agents were provided in the form of a bulk powder. Anaerobic incubation was performed using a disposable O 2 absorbing and CO 2 generating agent (AnaeroPack ® ; Mitsubishi Gas Chemical Co.). The inocula (ca. 10 4 colony forming units/spot) were plated using a multipoint incubator (Sakuma Co.). MIC were defined as the lowest concentration of agent inhibiting visible growth.Productivity of Verotoxin The production of verotoxin (Shiga-like toxin) was determined by VTEC-RPLA SEIKEN (De...

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Uptake of [3H]-fosfomycin into cells of enterohemorrhagic Escherichia coli O157 and E. coli non-O157

Cited by 7 publications

References 8 publications

Emergence of Fosfomycin-Resistant Isolates of Shiga-Like Toxin-Producing Escherichia coli O26

Emergence of Fosfomycin-Resistant Isolates of Shiga-Like Toxin-Producing Escherichia coli O26

Synthetic Methods for Azaheterocyclic Phosphonates and Their Biological Activity

In Vitro Susceptibility of Four Serotypes of Enterohaemorrhagic Escherichia coli to Antimicrobial Agents.

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