Uptake mechanisms of polychlorinated biphenyls in Cucurbita pepo via xylem sap containing major latex-like proteins

Goto, Junya; Iwabuchi, Aya; Yoshihara, Ryouhei; Kodama, Noriko; Matsui, Teppei; Hirota, Matashi; Eun, Heesoo; Inui, Hideyuki

doi:10.1016/j.envexpbot.2019.03.019

Cited by 26 publications

(35 citation statements)

References 31 publications

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“…Proteins were separated using a 15% polyacrylamide gel and transferred to a PVDF-Plus membrane (Maine Manufacturing, LLC, Sanford, ME, USA) to detect the MLPs using anti-MLP-PG1 and anti-MLP-GR3 antibodies. 9) 2. Uptake of pyrene by C. pepo incubated at different cultivation temperatures 2.1.…”

Section: Western Blotting Of Mlps In the Roots And Xylem Sapmentioning

confidence: 99%

“…9) Recombinant MLPs also bound to 17β-estradiol, 4-toctylphenol, and dieldrin. 9) In fact, zucchini plants have been reported to accumulate PCBs 10,11) and dieldrin 1,12) in their shoots when cultivated in contaminated soil. Many plant species have MLP genes in their genomes: for example Cucumis sativus and Arabidopsis thaliana genomes contain 38 and 25 genes, respectively.…”

Section: Introductionmentioning

confidence: 98%

“…8) Three MLP genes (MLP-PG1, MLP-GR1, and MLP-GR3) were mainly expressed in the roots, and the MLPs in the roots and xylem sap bound to the PCBs. 8,9) MLP-PG1 and MLP-GR3 apparently serve different functions, probably including defense responses to pathogens, as indicated by their different levels of gene expression and protein accumu-lation. 9) Recombinant MLPs also bound to 17β-estradiol, 4-toctylphenol, and dieldrin.…”

Section: Introductionmentioning

confidence: 99%

“…8,9) MLP-PG1 and MLP-GR3 apparently serve different functions, probably including defense responses to pathogens, as indicated by their different levels of gene expression and protein accumu-lation. 9) Recombinant MLPs also bound to 17β-estradiol, 4-toctylphenol, and dieldrin. 9) In fact, zucchini plants have been reported to accumulate PCBs 10,11) and dieldrin 1,12) in their shoots when cultivated in contaminated soil.…”

Section: Introductionmentioning

confidence: 99%

“…PG and MG cultivars have different accumulation abilities for DDE, 25) dioxins, and PCBs. 9,11) Furthermore, we investigated the changes in the pyrene contamination level in zucchini plants incubated in experimentally polluted soil. Pyrene, which is a hydrophobic pollutant, was used as a model POP compound because it is highly accumulated in the shoots of zucchini plants with high POP accumulation ability 26) and is simply quantified using its fluorescence.…”

Section: Introductionmentioning

confidence: 99%

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High temperatures promote the uptake of hydrophobic pollutants by <i>Cucurbita pepo via</i> altered gene expression levels of major latex-like proteins

et al. 2020

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Cucurbitaceae family members are accumulators of hydrophobic pollutants. Such pollutants have been detected in cucurbits at levels above the maximum residue limit. Since major latex-like proteins (MLPs) are involved in hydrophobic pollutant uptake, changes in MLP expression can increase or decrease contamination. MLP expression levels were altered in the roots of Cucurbita pepo 'Magda, ' and MLP-PG1 was detected in the xylem sap of Magda when cultivated at a high temperature (35°C). Day length also influenced MLP expression levels but only induced minor changes in the amount of MLPs. The concentration of pyrene, a hydrophobic pollutant, significantly increased with increasing MLP levels in the xylem sap of Magda when cultivated at 35°C. Thus, high temperatures promote the pollution of cucurbits by hydrophobic pollutants. These results can be used to develop novel techniques to reduce crop contamination and establish efficient phytoremediation.

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Section: Western Blotting Of Mlps In the Roots And Xylem Sapmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

High temperatures promote the uptake of hydrophobic pollutants by <i>Cucurbita pepo via</i> altered gene expression levels of major latex-like proteins

et al. 2020

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MLP-PG1, a major latex-like protein identified in Cucurbita pepo, confers resistance through the induction of pathogenesis-related genes

Fujita

Asuke

Isono

et al. 2021

Planta

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Genome-wide identification and characterization of major latex-like protein genes responsible for crop contamination in Cucurbita pepo

et al. 2022

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BackgroundZucchini plants (Cucurbita pepo) accumulate persistent organic pollutants (POPs) at high concentrations in their aerial parts, and major latex-like proteins (MLPs) play crucial roles in their accumulation. MLPs bind to POPs in root cells, MLP-POP complexes are then translocated into xylem vessels, and POPs are transported to the aerial parts. We previously identified three CpMLP genes (MLP-PG1, MLP-GR1, and MLP-GR3) as transporting factors for POPs; however, other studies have shown that the genomes of several plant species contain more than 10 MLP genes, thus, further MLP genes responsible for POP accumulation may have been overlooked. Methods and ResultsHere, we investigated the number of CpMLP genes by performing a hidden Markov model search against the C. pepo genome database and characterized their effects on POP accumulation by performing the expression analysis in the organs and in silico structural analysis. The C. pepo genome contained 21 CpMLP genes, and several CpMLP genes, including MLP-PG1 and MLP-GR3, were highly expressed in roots. 3D structural prediction showed that all examined CpMLPs contained a cavity with a hydrophobic region, which facilitated binding to POPs. ConclusionsThe present study provides insights regarding CpMLP genes responsible for POP accumulation.

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Uptake mechanisms of polychlorinated biphenyls in Cucurbita pepo via xylem sap containing major latex-like proteins

Cited by 26 publications

References 31 publications

High temperatures promote the uptake of hydrophobic pollutants by <i>Cucurbita pepo via</i> altered gene expression levels of major latex-like proteins

High temperatures promote the uptake of hydrophobic pollutants by <i>Cucurbita pepo via</i> altered gene expression levels of major latex-like proteins

MLP-PG1, a major latex-like protein identified in Cucurbita pepo, confers resistance through the induction of pathogenesis-related genes

Genome-wide identification and characterization of major latex-like protein genes responsible for crop contamination in Cucurbita pepo

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