Uptake Mechanism of Oppositely Charged Fluorescent Nanoparticles in HeLa Cells

Dausend, Julia; Musyanovych, Anna; Dass, Martin; Walther, Paul; Schrezenmeier, Hubert; Landfester, Katharina; Mailänder, Volker

doi:10.1002/mabi.200800123

Cited by 260 publications

(245 citation statements)

References 34 publications

Supporting

Mentioning

228

Contrasting

Unclassified

Order By: Relevance

“…8,9 Previous studies have shown that the uptake of NPs by living cells is affected by NP properties such as size [10][11][12][13] and surface. [14][15][16] However, NPs dispersed in a biological fluid are rapidly covered by biomolecules, such as proteins and lipids, forming a biomolecular 'corona' that effectively screens the bare NP surface. [17][18][19][20][21][22] When cells are exposed to NPs it is therefore, in realistic circumstances, typically not the bare NP surface that interacts with the cell but the NP-biomolecular corona complex.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle Adhesion to the Cell Membrane and Its Effect on Nanoparticle Uptake Efficiency

et al. 2013

View full text Add to dashboard Cite

ABSTRACT:The interactions between nano-sized particles and living systems are commonly mediated by what adsorbs to the nanoparticle in the biological environment, its "biomolecular corona", rather than the pristine surface. Here we characterise the adhesion towards the cell membrane of nanoparticles of different material and size, and study how this is modulated by the presence or absence of a corona on the nanoparticle surface. The results are corroborated with adsorption to simple model supported lipid bilayers using a quartz crystal microbalance. We conclude that the adsorption of proteins on the nanoparticle surface strongly reduces nanoparticle adhesion in comparison to what is observed for the bare material. Nanoparticle uptake is described as a two-step process, where the nanoparticles initially adhere to the cell membrane and subsequently are internalised by the cells via energy-dependent pathways. The lowered adhesion in the presence of proteins thereby causes a concomitant decrease in nanoparticle uptake efficiency. The presence of a biomolecular corona may confer specific interactions between the nanoparticle-corona complex and the cell surface, including triggering of regulated cell uptake. An important effect of the corona is, however, a reduction in the purely unspecific interactions between the bare material and the cell membrane, which in itself disregarding specific interactions, causes a decrease in cellular uptake. We suggest that future nanoparticle-cell studies include, together with characterisation of size, charge and dispersion stability, an evaluation of the adhesion properties of the material to relevant membranes.

show abstract

Section: Introductionmentioning

confidence: 99%

Nanoparticle Adhesion to the Cell Membrane and Its Effect on Nanoparticle Uptake Efficiency

et al. 2013

View full text Add to dashboard Cite

show abstract

“…About 20 % of the endocytosis of positively charged particles is inhibited by chlorpromazine. 39 Clathrin-coated pits (Fig. 2C) only play a minor role in the uptake of positively charged NPs, and have no effect on the endocytosis of negatively charged NPs.…”

Section: Np Uptake Into Cellsmentioning

confidence: 99%

Big Signals from Small Particles: Regulation of Cell Signaling Pathways by Nanoparticles

Rauch¹,

Kölch²,

et al. 2013

View full text Add to dashboard Cite

Introduction 3391 2. Medical Applications of Nanomaterials 3391 2.1. Use of Nanomaterials in Diagnostic Applications 3392 3. Interaction of Nanomaterials with Living Cells 3392 3.1. Role of Physicochemical NP Properties in the Interaction between NPs and Biological Systems 3393 3.2. NP Uptake into the Cells 3393 3.2.1. Role of Size and Shape in NP Uptake 3393 3.2.2. Role of Surface Charge and Protein Corona on NP Uptake 3395 3.3. Activation of Signal Processing Pathways by NPs 3397 3.3.1. Interaction of NPs with Cell Membrane Receptors 3397 3.3.2. Role of Oxidative Stress and ROS in NP-Induced Signaling 3398 3.3.3. NPs Induce Cell Death Pathways 3400 3.3.4. Activation of Mechanotransduction Receptors by Magnetic NPs 3400 4. Magnetic NPs and Cell Signaling 3400 4.1. Controlled Activation of Cell Surface Receptors by Magnetic NPs 3400 4.1.1. Activation of Signal Transduction Receptors by Magnetic NPs 3400 4.1.2. Activation of Mechanotransduction Receptors and Mechanosensitive Signaling Pathways by Magnetic NPs 3400 4.2. Pathway Activation by Magnetic-NP-Induced Hyperthermia 3401 4.2.1. Molecular Targets of Hyperthermia: Heat Shock Proteins and p53 3401 5. Conclusions 3402 Author Information 3402 Corresponding Author 3402 Present Address 3402 Notes 3402 Biographies 3402 Acknowledgments 3403 References 3403

show abstract

“…It was evaluated which endocytotic mechanisms are involved in the uptake of well-defined positively and negatively charged 100-nm fluorescent polystyrene nanoparticles into HeLa cells. 83 To study the uptake mechanism, nanoparticles were incubated with HeLa cells in the presence or absence of drugs known to inhibit various factors in endocytosis. The amount of endocytosed particles was quantified using fluorescent particles (see below) by flow cytometry, and the intracellular localization was confirmed by confocal laser scanning and transmission electron microscopy (TEM).…”

Section: Functionalization Of Nanoparticles and Nanocapsulesmentioning

confidence: 99%

“…18 To investigate the uptake path in HeLa cells, positively and negatively charged PS nanoparticles of the same size were applied in combination with selective inhibitors for different uptake mechanisms. 83 Here, the different uptake mechanisms known so far (e.g., clathrin or caveolin mediated endocytosis, see Fig. 13) can be investigated.…”

Section: Radioactive Isotopes and Fluorescent Dyes As Markersmentioning

confidence: 99%

See 1 more Smart Citation

From polymeric particles to multifunctional nanocapsules for biomedical applications using the miniemulsion process

Landfester

Musyanovych

Mailänder

2009

J. Polym. Sci. A Polym. Chem.

Self Cite

160

122

View full text Add to dashboard Cite

ABSTRACT:The miniemulsions process represents a versatile tool for the formation of polymeric nanoparticles consisting of different kinds of polymer as obtained by a variety of polymerization types ranging from radical, anionic, cationic, enzymatic polymerization to polyaddition, and polycondensation. The process perfectly allows the encapsulation of hydrophilic and hydrophobic liquids and solids in polymeric shells, molecularly dissolved dyes or other components. In combination with a specific functionalization of the nanoparticles' or nanocapsules' surfaces and the possibility to release substances in a defined way from the interior, complex nanoparticles or nanocapsules are obtained, which are ideally suited for application in biomedical application as marker and targeted drug-delivery system. V C 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 2010

show abstract

Uptake Mechanism of Oppositely Charged Fluorescent Nanoparticles in HeLa Cells

Cited by 260 publications

References 34 publications

Nanoparticle Adhesion to the Cell Membrane and Its Effect on Nanoparticle Uptake Efficiency

Nanoparticle Adhesion to the Cell Membrane and Its Effect on Nanoparticle Uptake Efficiency

Big Signals from Small Particles: Regulation of Cell Signaling Pathways by Nanoparticles

From polymeric particles to multifunctional nanocapsules for biomedical applications using the miniemulsion process

Contact Info

Product

Resources

About