Uptake by and Fate of Lysozyme in Roots of Iasione montana (Campanulaceae)

Abstract

A basic protein, lysozyme, labeled with fluorescein isothiocyanate, is readily taken up by roots of lasione montana. Most of the protein taken up is tightly bound to cell walls of the roots. Fluorescent protein is diluted in the growing region of a root as cells elongate and divide. Fluorescence remains in mature nongrowing regions and root cap cells for one to two weeks. Redistribution and translocation of the protein within the root is minimal or nil. A layer of chloroform‐soluble material that prevents lysozyme from interacting with stem cell walls exposed to fluorescent lysozyme was found on stems of germinating Iasione montana.

Impurity effects of lysozyme molecules specifically labeled with a fluorescent reagent on the crystallization of tetragonal and monoclinic lysozyme crystals

“…As to the second requirement, fluorescent labels offer significant advantages: one can detect fluorescent-labeled impure proteins present at concentrations on the order of 0.1 mM [21]. This high sensitivity is essential to measure the concentration and spatial distribution of impure proteins in a target crystal [21,22], as used widely in other fields from long ago [23][24][25][26].…”