Uptake and processing of liposomal phospholipids by Kupffer cells <i>in vitro</i>

Dijkstra, J.W.; Galen, Mieke van; Regts, Djoeke; Scherphof, Gerrit L.

doi:10.1111/j.1432-1033.1985.tb08851.x

Cited by 64 publications

(23 citation statements)

References 44 publications

(32 reference statements)

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“…Liposomes are preferentially trapped by phagocytic cells in the reticuloendothelial organs such as the liver and spleen (3,9,10). However, in this study we observed no such greater distribution of Lip-RFP than of free RFP in the liver, although some prolonged retention of Lip-RFP in the spleen was noted.…”

Section: Discussioncontrasting

confidence: 53%

“…Since liposomes are preferentially taken up by reticuloendothelial cells (3,9,10), liposomal encapsulation of a given agent should result in greater availability of the agent to parasites present in phagocytic cells. The entrapment of certain antibiotics in liposomes increases their activity against bacteria phagocytosed into macrophages in in vitro culture systems (3,5,7).…”

mentioning

confidence: 99%

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Therapeutic efficacy of liposome-entrapped rifampin against Mycobacterium avium complex infection induced in mice

Saito

Tomioka

1989

Antimicrob Agents Chemother

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Liposome-entrapped rifampin (RFP) was examined for therapeutic efficacy against experimental infection induced in mice by the Mycobacterium avium complex. Intraperitoneal injections (once daily, six times weekly) of liposome-entrapped RFP led to a greater reduction in bacterial growth in the lungs and spleen of infected mice than did free RFP alone. Liposome-entrapped RFP given to mice via the intramuscular or subcutaneous route failed to show such an increased therapeutic efficacy. RFP entrapped in the lipid layer of liposomal vesicles exhibited a level of therapeutic activity similar to that seen with RFP encapsulated in the inner solute of the vesicles. Entrapment of RFP in liposomal vesicles increased incorporation of the drug into host peritoneal macrophages and increased the activity of the agent against M. avium complex phagocytosed into the macrophages.

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Section: Discussioncontrasting

confidence: 53%

mentioning

confidence: 99%

Therapeutic efficacy of liposome-entrapped rifampin against Mycobacterium avium complex infection induced in mice

Saito

Tomioka

1989

Antimicrob Agents Chemother

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“…On the other hand, we did not directly examine the disposition of the DPPC, DHSG, PEG-DSPE, and H12-PEG-Glu2C18 in H12-(ADP)-liposomes. Previous reports have shown that the phospholipids in liposomes are metabolized in the MPS and reused as cell membranes or are excreted into the bile (Dijkstra et al, 1985;Verkade et al, 1991). Therefore, it is also possible that phospholipids in H12-(ADP)-liposome are also metabolized and excreted in the same manner as the other liposome components, as mentioned above.…”

mentioning

confidence: 80%

Pharmacokinetic Study of the Structural Components of Adenosine Diphosphate-Encapsulated Liposomes Coated with Fibrinogenγ-Chain Dodecapeptide as a Synthetic Platelet Substitute

et al. 2013

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Fibrinogen g-chain (dodecapeptide HHLGGAKQAGDV, H12)-coated, ADP-encapsulated liposomes [H12-(ADP)-liposomes] were developed as a synthetic platelet alternative that specifically accumulates at bleeding sites as the result of interactions with activated platelets via glycoprotein IIb/IIIa and augments platelet aggregation by releasing ADP. The aim of this study is to characterize the pharmacokinetic properties of H12-(ADP)-liposomes and structural components in rats, and to predict the blood retention of H12-(ADP)-liposomes in humans. With use of H12-(ADP)-liposomes in which the encapsulated ADP and liposomal membrane cholesterol were radiolabeled with 14 C and 3 H, respectively, it was found that the time courses for the plasma concentration curves of 14 C and 3 H radioactivity showed that the H12-(ADP)-liposomes remained intact in the blood circulation for up to 24 hours after injection, and were mainly distributed to the liver and spleen. However, the 14 C and 3 H radioactivity of H12-(ADP)-liposomes disappeared from organs within 7 days after injection. The encapsulated ADP was metabolized to allantoin, which is the final metabolite of ADP in rodents, and was mainly eliminated in the urine, whereas the cholesterol was mainly eliminated in feces. In addition, the half-life of the H12-(ADP)-liposomes in humans was predicted to be approximately 96 hours from pharmacokinetic data obtained for mice, rats, and rabbits using an allometric equation. These results suggest that the H12-(ADP)-liposome has potential with proper pharmacokinetic and acceptable biodegradable properties as a synthetic platelet substitute.

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“…It is also well known from results of other in vitro studies that macrophages can take up liposomes very avidly (8,21,23,27). Uptake occurs predominantly by way of phagocytosis, followed by extensive degradation of the vesicles within the lysosomal compartment of the cells (9,24 (14). In contrast to the effect of 50 or 150 ,ug of free ampicillin per ml plus empty liposomes, a concentration of 50 ,g of free ampicillin plus 100 ,ug of liposome-entrapped ampicillin per ml killed 99% of the intracellular bacteria.…”

Section: Discussionmentioning

confidence: 99%

Effect of liposome-entrapped ampicillin on survival of Listeria monocytogenes in murine peritoneal macrophages

Bakker-Woudenberg¹,

Lokerse²,

Berg³

et al. 1986

Antimicrob Agents Chemother

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The effect of liposomal encapsulation of ampicillin on the antibacterial activity against intracellular Listeria monocytogenes was studied by comparing survival of L. monocytogenes within peritoneal mouse macrophages in the presence of free ampicillin alone or in combination with liposome-entrapped ampicillin. In the presence of 50 ,ug of free ampicillin per ml of the incubation medium, intracellular growth of L. monocytogenes was still observed, although less as compared with intracellular growth in the absence of ampicillin. At a concentration of 50 ,ug of free ampicillin plus 100 ,ug of liposome-entrapped ampicillin per ml, 99% of the intracellular bacteria were killed. On the other hand, a concentration of 150 ,ug of free ampicillin per ml plus empty liposomes only inhibited intracellular bacterial growth, and the bacteria were not killed. In addition, empty liposomes at a concentration of 1 ,umol of lipid per ml had no effect on intracellular bacterial growth. In broth, liposome-entrapped ampicillin at a concentration of 100 ,ug/mI was not bactericidal for L. monocytogenes, indicating that significant leakage of ampicillin from the liposomes with subsequent killing of the bacteria by the free drug did not occur. Therefore, we concluded that liposomal encapsulation of ampicillin results in an increased availability of the antibiotic for the intracellular bacteria.

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Uptake and processing of liposomal phospholipids by Kupffer cells in vitro

Cited by 64 publications

References 44 publications

Therapeutic efficacy of liposome-entrapped rifampin against Mycobacterium avium complex infection induced in mice

Therapeutic efficacy of liposome-entrapped rifampin against Mycobacterium avium complex infection induced in mice

Pharmacokinetic Study of the Structural Components of Adenosine Diphosphate-Encapsulated Liposomes Coated with Fibrinogenγ-Chain Dodecapeptide as a Synthetic Platelet Substitute

Effect of liposome-entrapped ampicillin on survival of Listeria monocytogenes in murine peritoneal macrophages

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