Uptake and intracellular release kinetics of liposome formulations in glioma cells

Bellavance, Marc-André; Poirier, Marie-Belle; Fortin, David

doi:10.1016/j.ijpharm.2010.05.017

Cited by 42 publications

(20 citation statements)

References 61 publications

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“…44–46 In addition, it is well known that the transfection efficiency of lipoplex can be boosted by incorporation of DOPE as a helper lipid in the formulation. 47–49 …”

Section: Resultsmentioning

confidence: 99%

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Effective Targeted Gene Delivery to Dendritic Cells via Synergetic Interaction of Mannosylated Lipid with DOPE and BCAT

et al. 2012

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The efficient delivery of plasmids encoding antigenic determinants into dendritic cells (DCs) that control immune response is a promising strategy for rapid development of new vaccines. In this study, we prepared a series of targeted cationic lipoplex based on two synthetic lipid components, mannose-poly(ethylene glycol, MW3000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (Mannose-PEG3000-DSPE) and O-(2R-1,2-di-O-(1'Z,9'Z-octadecadienyl)-glycerol)-3-N-(bis-2-aminoethyl)-carbamate (BCAT), that were formulated with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) for evaluation as non-viral vectors for transgene expression in DCs. First, we optimized the N:P ratio for maximum transfection and then screened the effects of mannose targeting for further enhancement of transfection levels. Our results indicate that efficient delivery of gWIZ GFP plasmid into DCs was observed for mannose compositions of ~10%, whereas low transfection efficiencies were observed with non-targeted formulations. Mannose-targeted lipofectamine complexes also showed high GFP expression levels in DCs relative to non-targeted lipofectamine controls. The best transfection performance was observed using 10 mol % Mannose-PEG3000-DSPE, 60 mol% BCAT, and 30 mol % DOPE, indicating that the most efficient delivery into DCs occurs via synergistic interaction between mannose targeting and acid-labile, fusogenic BCAT:DOPE formulations. Our data suggest that mannose-PEG3000-DSPE:BCAT:DOPE formulations may be effective gene delivery vehicles for the development of DC-based vaccines.

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“…44–46 In addition, it is well known that the transfection efficiency of lipoplex can be boosted by incorporation of DOPE as a helper lipid in the formulation. 47–49 …”

Section: Resultsmentioning

confidence: 99%

“…These results are consistent with previous observations that cationic liposomes containing significant DOPE loadings display similarly high levels of transfection. 47,50–53 …”

Section: Resultsmentioning

confidence: 99%

Effective Targeted Gene Delivery to Dendritic Cells via Synergetic Interaction of Mannosylated Lipid with DOPE and BCAT

et al. 2012

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“…3. Quantum dots: Quantum dots are another group of nanotechnology-made fluorescent dyes 28,32 . These nanoscale semiconductor crystals have special fluorescent properties including minimal photo bleaching, optimal stability, high signal to noise ratio and broad absorption spectrum with very narrow but size-dependant tunable emission spectrum.…”

Section: Optical Imagingmentioning

confidence: 99%

“…These calcium channels cause an imbalance in calcium homeostasis, that ends in oxidative stress. In addition, the membrane integrated Aβ can chemically interact through (amino acid) Methionine (not shown) with the membrane lipid molecules and the resultant lipid peroxidation produces 4-hydroxy-2-nonenal (4HNE) [32] . Such an interaction leads to membrane disruption and production of reactive oxygen species and finally oxidative stress in the involved brain tissue.…”

Section: Nanotechnology Based Treatment For Alzheimer's Disease:-mentioning

confidence: 99%

“…1. Polymeric nanoparticles: Nanoparticles (NPs) are, colloidal particles, less than 1000 nm, that can be used for better drug delivery and prepared either by encapsulating the drug within a vesicle and or by dispersing the drug molecules within a matrix 32 . Nanoparticulate drug delivery systems have been extensively studied in recent years for spatial and temporal delivery, especially in tumour and brain targeting.…”

Section: Nanoformulations Investigated:-mentioning

confidence: 99%

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The Aging Brain: Nanotechnology Solutions With Diagnostic Methods and Recent Advances.

Habeeb¹,

Noor.²

2016

IJAR

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Alzheimer's disease, Nanotechnology, Blood brain barrier (BBB) Alzheimer's disease (AD) is the leading cause of dementia is a devastating degenerative disease of brain that results in progressive cognitive impairment and functional disability. AD lacks definite diagnostic approaches and effective cure at the present. Moreover, the currently available diagnostic tools are not sufficient for an early screening of AD in order to start preventive approaches. Recently the emerging field of nanotechnology has promised new techniques to solve some of the AD challenges. In this review, we present the promises that nanotechnology brings in research on the AD diagnosis ,Proposed invitro and invivo nanodiagnostic approaches, Nanoformulations investigated and therapy through nanotechnology. Applications of nanotechnology in AD therapy including neuroprotections against oxidative stress and anti-amyloid therapeutics, neuroregeneration and drug delivery beyond the blood brain barrier (BBB) are discussed and analyzed. All these applications could improve the treatment approach of AD and other neurodegenerative diseases. The complete cure of AD may become feasible by a combination of nanotechnology and some other novel approaches, like stem cell technology.Copy Right, IJAR, 2016,. All rights reserved. …………………………………………………………………………………………………….... Introduction:-Alzheimer's disease (AD) is an acquired disorder of cognitive and behavioral impairment that is an incurable disease with a long and progressive course. In AD, plaques develop in the hippocampus, a structure deep in the brain that helps to encode memories, and in other areas of the cerebral cortex that are used in thinking and decision making. Alois Alzheimer defined "senile dementia" more than a century ago with a remarkable accuracy 1,2 . It is now known as the Alzheimer's Disease (AD), and is the main cause of the dementia syndrome 3 . The incidence and prevalence of AD increase with age. Since the elderly population is growing worldwide, AD is quickly becoming one of the major universal healthcare problems 4 . Today, however, there are neither precise diagnostic approaches nor effective therapeutic agents available for Alzheimer's disease. The degeneration of nervous tissue begins many years to even decades before the patient experiences any of Alzheimer's disease symptoms 5,7 . On the other hand, the currently available therapeutics for AD, only act to lower its symptoms 8 . Therefore, whether the disease is diagnosed early enough or not, the conventional medical approaches are incapable of complete cessation or reversal of the disease progress. The uncovering potentials of nanotechnology have been opening new chapters in many aspects of our lives, specially diagnosis and treatment of human diseases 9,11 . Through nanotechnology, the controllable Corresponding Author:-Ayesha Habeeb. Address:-Deccan school of pharmacy, Department of pharmaceutics,dar us salam, aghapura, nampally, hyderabad telangana, India. ISSN: 2320-5407Int. J. Adv. Res. 4(10), 877-888 878 produc...

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Recent Advances with Liposomes as Drug Carriers

Sriraman

Torchilin

2014

Advanced Biomaterials and Biodevices

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Uptake and intracellular release kinetics of liposome formulations in glioma cells

Cited by 42 publications

References 61 publications

Effective Targeted Gene Delivery to Dendritic Cells via Synergetic Interaction of Mannosylated Lipid with DOPE and BCAT

Effective Targeted Gene Delivery to Dendritic Cells via Synergetic Interaction of Mannosylated Lipid with DOPE and BCAT

The Aging Brain: Nanotechnology Solutions With Diagnostic Methods and Recent Advances.

Recent Advances with Liposomes as Drug Carriers

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