Uptake and intracellular handling of iron from transferrin and iron chelates by mitogen stimulated mouse lymphocytes

Djeha, Abdelhakim; Brock, Jeremy H.

doi:10.1016/0167-4889(92)90062-g

Cited by 39 publications

(20 citation statements)

References 20 publications

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“…However, we noted an increased proliferation capacity of T lymphocytes after incubation with a VSOP concentration of 1 mM Fe/ml. Our findings are in line with studies showing that moderate concentrations of iron have a growth-promoting effect on lymphocytes whereas high iron concentrations may reduce cell proliferation (Djeha and Brock, 1992). Yet, in our cultures, this increased relative CD4+ T cell proliferation may be linked to the selectively enhanced cell death of CD8+ T cells observed at the same time.…”

Section: Discussionsupporting

confidence: 93%

Electrostatically stabilized magnetic nanoparticles – an optimized protocol to label murine T cells for in vivo MRI

et al. 2011

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We present a novel highly efficient protocol to magnetically label T cells applying electrostatically stabilized very small superparamagnetic iron oxide particles (VSOP). Our long-term aim is to use magnetic resonance imaging (MRI) to investigate T cell dynamics in vivo during the course of neuroinflammatory disorders such as experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. Encephalitogenic T cells were co-incubated with VSOP, or with protamine-complexed VSOP (VProt), respectively, at different conditions, optimizing concentrations and incubation times. Labeling efficacy was determined by atomic absorption spectrometry as well as histologically, and evaluated on a 7 T MR system. Furthermore, we investigated possible alterations of T cell physiology caused by the labeling procedure. T cell co-incubation with VSOP resulted in an efficient cellular iron uptake. T2 times of labeled cells dropped significantly, resulting in prominent hypointensity on T2*-weighted scans. Optimal labeling efficacy was achieved by VProt (1 mM Fe/ml, 8 h incubation; T2 time shortening of ∼80% compared to untreated cells). Although VSOP promoted T cell proliferation and altered the ratio of T cell subpopulations toward a CD4+ phenotype, no effects on CD4 T cell proliferation or phenotypic stability were observed by labeling in vitro differentiated Th17 cells with VProt. Yet, high concentrations of intracellular iron oxide might induce alterations in T cell function, which should be considered in cell tagging studies. Moreover, we demonstrated that labeling of encephalitogenic T cells did not affect pathogenicity; labeled T cells were still capable of inducing EAE in susceptible recipient mice.

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Section: Discussionsupporting

confidence: 93%

Electrostatically stabilized magnetic nanoparticles – an optimized protocol to label murine T cells for in vivo MRI

et al. 2011

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“…Oversaturation of transferrin and high ferritin are known to impair the immune response [34,35]. In our previous study, we showed that non-responders to an influenza vaccine had very high levels of ferritin compared to responders [median 821 μg/L (nonresponders) versus 571 (responders)] [12].…”

Section: Discussionmentioning

confidence: 99%

Previous Vaccination and Age are More Important Predictors of Immune Response to Influenza Vaccine than Inflammation and Iron Status in Dialysis Patients

Eiselt¹,

Kielberger²,

Rajdl³

et al. 2016

Kidney Blood Press Res

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Background/Aims: The immune response to influenza vaccine may be influenced by many factors, e.g. age, comorbidities or inflammation, and iron status. Methods: We studied the vaccine-induced production of hemagglutination-inhibition antibodies (HI) in 133 hemodialysis patients (HD) and 40 controls. To identify variables associated with the immune response, uni- and multivariate regression analyses were performed with seroconversion in HI titers as a dependent variable, with demographics, comorbidities, previous vaccination, inflammation, and iron status as independent variables. Results: Seroconversion rates were lower in HD than in controls [43% versus 73% (H1N1 strain; p < 0.05); 43% versus 53% (H3N2; P=NS); 36% versus 62% (B; p < 0.05)]. In both HD and control groups, the predictors of the inferior HI production were pre-vaccination seroprotection, vaccination in the previous season, and old age. We did not find associations between seroconversion rates and inflammation and iron status in the studied populations. This was also true for a subanalysis of patients without pre-vaccination seroprotection. Conclusion: The influenza vaccine-induced antibody production was lower in HD than in controls and was independent of inflammation and iron status in both groups. Besides dependence on dialysis, the variables associated with inferior seroconversion rates included pre-vaccination seroprotection, previous vaccination, and old age.

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“…It has been estimated that maturation from the early pro-B stage (the point at which RAG2 Ϫ/Ϫ mice are blocked) to the large pre-B stage (where VDJ H recombination is complete and cells begin to express the pre-B-cell receptor) requires approximately 6 cell divisions. [48][49][50] Perhaps B cells cannot effectively sequester storage iron 51 or cannot otherwise obtain the iron necessary for these divisions in the absence of TfR.…”

Section: Discussionmentioning

confidence: 99%

Transferrin receptor 1 is differentially required in lymphocyte development

Ned

Swat

Andrews

2003

Blood

102

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Transferrin receptor (TfR) facilitates cellular iron uptake by mediating endocytosis of its ligand, iron-loaded transferrin. Although TfR is widely believed to be important for iron acquisition by all mammalian cells, direct experimental evidence is lacking. We have previously shown that mouse embryos homozygous for a disrupted transferrin receptor allele (TfR ؊/؊ ) die of anemia before embryonic day 12.5, although most other embryonic tissues appear to be developing normally. Here, we have investigated the importance of TfR postnatally, by using TfR ؊/؊ embryonic stem cells to produce chimeric animals. We find that TfR ؊/؊ embryonic stem cells give rise to most tissues and organs, but do not contribute to hematopoietic tissues on a wild-type C57BL/6J background, indicating that both adult erythropoiesis and lymphopoiesis re-

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Uptake and intracellular handling of iron from transferrin and iron chelates by mitogen stimulated mouse lymphocytes

Cited by 39 publications

References 20 publications

Electrostatically stabilized magnetic nanoparticles – an optimized protocol to label murine T cells for in vivo MRI

Electrostatically stabilized magnetic nanoparticles – an optimized protocol to label murine T cells for in vivo MRI

Previous Vaccination and Age are More Important Predictors of Immune Response to Influenza Vaccine than Inflammation and Iron Status in Dialysis Patients

Transferrin receptor 1 is differentially required in lymphocyte development

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