Uptake and depuration of Ag nanoparticles <i>versus</i> Ag ions by zebrafish through dietary exposure: characterization of Ag nanoparticle formation and dissolution <i>in vivo</i> and toxicokinetic modeling

Yang, Jung Wei; Hou, Wen Che

doi:10.1039/d2en00113f

Cited by 2 publications

(1 citation statement)

References 50 publications

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“…In our tests, 4.7% of AgNPs was dissolved to Ag + at 50 μg/L and 4.3% for the 500 μg/L AgNP treatment (Figure a), indicating the low solubility of Ag + in AgNPs. Sotiriou et al found that Ag + dissolution occurred rapidly within 5 min, which was far shorter than the exposure time of 24 h. A few earlier studies demonstrated that the rates of uptake of Ag + and AgNPs were comparable in zebrafish. − On the basis of the percentage of the Ag dissolution rate and their comparable uptake rates in zebrafish, AgNPs contributed at least 20 times more than the released Ag + . Therefore, we focused primarily on the biological toxicity caused by the AgNP itself and ignored the released trace of Ag + in the medium.…”

Section: Resultsmentioning

confidence: 99%

Nanoscale Whole-Body Expansion Microscopy Revealed the Early Skeletal Developmental Malformation Induced by Silver Nanoparticles

Wang

2023

Environ. Sci. Technol. Lett.

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The widely used silver nanoparticles (AgNPs) can cause damage to skeletal development in aquatic organisms, which has a significant impact on their survival and reproduction. We hypothesized that the biological toxicity of AgNPs itself may be the culprit for early skeletal developmental malformation. In this study, we achieved the first nanoscale visualization of the notochord and skeletal muscle anatomy affected by exposure to AgNPs through a novel whole-body expansion microscopy technique (whole-ExM). This technique allows the whole zebrafish to be expanded equally in three dimensions by ∼4.5 times, enabling nanoscale resolution imaging without complex and expensive equipment. Our results suggested that AgNPs caused curvature deformation of the notochord as well as reduced and loosely arranged skeletal muscle. In addition, we demonstrated that the main source of Ag + was the dissolution of AgNPs in vivo. Compared with that of the released Ag + , the developmental toxicity caused by AgNPs themselves was non-negligible. This work achieved cellular-level visualization of living animals and overcame the limitations of previous macroscopic or microscopic consideration of the toxicity of AgNPs. The study provided a new perspective for assessing the toxicity of nanomaterials by using whole-ExM to achieve ultraclear nanoscale imaging of biological organs.

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