Uptake and Biocompatibility of Functionalized Poly(vinylalcohol) Coated Superparamagnetic Maghemite Nanoparticles by Synoviocytes In Vitro

Abstract

Superparamagnetic iron oxide nanoparticles (SPION) were coated with either Polyvinyl alcohol (PVA) or Vinyl alcohol/vinyl amine copolymer and further functionalized with the fluorochromes Cy3.5 or Texas Red. A colloidally stable suspension of nanoparticles was incubated on sheep synovial cells in vitro for 3, 24, 72, and 120 hours. Nanoparticle internalization into synoviocytes as well as biocompatibility was visualized using light, fluorescence and confocal microscopy and fluorochrome labeled cells were quantified by flow cytometry. Data were analyzed by ANOVA factorial tests. Amino-PVA-SPION alone was detectable in cytoplasmic endosome-like structures after 3 hours of incubation but resulted in early cell death after 24 hours. Although amino-PVA-Cy3.5-SPION and PVA-TexasRed-SPION were taken up more slowly and less intensely, both labeled more than 80% of the cells in culture, but did not significantly change cell morphology or vitality at any time of evaluation in comparison to control cells. Results indicate that functionalized amino PVA-coated SPION are biocompatible, were successfully internalized by synoviocytes and hold promise for future biomedical applications utilizing magnetic drug targeting in joint disease.

Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

“…Consistently, we previously showed that more than 80% of the synoviocytes took up superparamagnetic nanoparticles within 24 h [54]. These cells also showed the highest increase in transfection efficiency (8.8-fold) after the exposure to static magnetic field.…”

“…Primary ovine synovial membrane cells (synoviocytes) were harvested and prepared for cell culture as previously described [54,55]. Briefly, synovial membranes of healthy sheep were collected under sterile conditions from the stifle joints immediately after slaughtering.…”

Optimal Conditions for Labelling of 3T3 Fibroblasts with Magnetoliposomes without Affecting Cellular Viability

“…Either the particles adhere to the periphery of the cell's plasma membrane, [37] or they are internalised. [38,39] After Prussian blue staining, the optical micrographs still clearly show the rather bulky nucleus, which tentatively suggests that internalisation occurred. Indeed, in case of a mere adhesion, one would expect the nucleus to be hidden under a uniform layer of Prussian blue stain, which evidently is not the case.…”

Magnetic nanoparticles for gene and drug delivery

“…The hydroxyl groups (-OH) on the polymer skeleton ensure the hydrophobic property of the coating, which resembles the surface chemistry of carbohydrates such as dextran. The use of PVA-coated magnetic nanoparticles in biomedical applications has been reported (Schulze et al 2006). This research group has demonstrated the internalization of PVA and PVA co-polymer-coated magnetic nanoparticles by synoviocytes and by cells of the synovial membrane in sheep (Schulze et al 2005).…”