Uptake and biochemical responses of mussels Mytilus galloprovincialis exposed to sublethal nickel concentrations

Attig, Hajer; Dagnino, A.; Negri, Alessandro; Jebali, Jamel; Boussetta, Hamadi; Viarengo, Aldo; Dondero, Francesco; Bannı, Mohamed

doi:10.1016/j.ecoenv.2010.08.007

Cited by 62 publications

(17 citation statements)

References 31 publications

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“…2), where the slope corresponded to the uptake rate constant, k u = 0.032 ± 0.005 L g -1 d -1 . This value was within the range reported for other aquatic organisms such as the marine mussel Mytilus galloprovinvialis or the freshwater insect Chaoborus flavicans, for which k u values were 0.047 or 0.019 L g -1 d -1 respectively (Attig et al, 2010;Ponton and Hare, 2010). The uptake rate constant for Ni was 35 times lower than one observed for Cd in G. pulex with k u = 0.46 L g -1 d -1 (Pellet et al, 2009).…”

Section: Saturation Of Metal Uptake and Binding Characteristicssupporting

confidence: 78%

Waterborne nickel bioaccumulation in Gammarus pulex: Comparison of mechanistic models and influence of water cationic composition

Lebrun¹,

Perret²,

Uher³

et al. 2011

Aquatic Toxicology

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The biodynamic and saturation models offer promising lines of enquiry to predict the bioaccumulation of metals by aquatic organisms. However, in order to construct these models, the accumulation strategies have to be defined for each metal/organism couple in controlled conditions. This study aims at modelling the waterborne bioaccumulation of Ni and the influence of the water's geochemical properties on this process in a crustacean that is widely distributed in Europe, Gammarus pulex. In the laboratory, G. pulex was exposed to several Ni concentrations (from 0.001 to 100 mg L(-1)) in aquatic microcosms. Our results show that G. pulex is very tolerant to Ni (LC50(48 h)=477 mg L(-1) Ni). Time course experiments enabled the construction of a biodynamic model by determining the uptake (k(u)) and elimination (k(e)) rate constants. When the exposure concentration exceeded 1 mg L(-1) Ni, the metal uptake reached a maximum due to a limited number of binding sites for Ni. Therefore, the organism's maximal capacity to accumulate the metal (B(max)) and the half-saturation constant (K) were determined to establish the saturation model. We showed that the two models are comparable for the lowest exposure concentrations (<1 mg L(-1) Ni), with k(u)/k(e)=B(max)/K. Then, the bioaccumulation of Ni was recorded in waters exhibiting various concentrations of three major ions (Na(+), Mg(2+) and Ca(2+)). Only Ca had an inhibitory effect on the Ni uptake. This study reports for the first time the bioaccumulation of Ni in G. pulex. Because of its high tolerance to Ni and its high capacity to accumulate this metal, this crustacean could be used as an indicator of Ni bioavailability in freshwaters.

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Section: Saturation Of Metal Uptake and Binding Characteristicssupporting

confidence: 78%

Waterborne nickel bioaccumulation in Gammarus pulex: Comparison of mechanistic models and influence of water cationic composition

Lebrun¹,

Perret²,

Uher³

et al. 2011

Aquatic Toxicology

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“…Mytilus spp. have been used extensively in biomonitoring projects through the application of a battery of physiological and cellular markers, providing evidence of a stress syndrome and demonstrating the biological risk associated with polluted environments [9,10]. Mussels are particularly useful in this context because they inhabit regions of different pollution status, accumulate xenobiotics, and are sessile.…”

Section: Introductionmentioning

confidence: 99%

Assessing the impact of Benzo[a]pyrene on Marine Mussels: Application of a novel targeted low density microarray complementing classical biomarker responses

Bannı¹,

Sforzini²,

Arlt³

et al. 2017

PLoS ONE

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Despite the increasing use of mussels in environmental monitoring and ecotoxicological studies, their genomes and gene functions have not been thoroughly explored. Several cDNA microarrays were recently proposed for Mytilus spp., but putatively identified partial transcripts have rendered the generation of robust transcriptional responses difficult in terms of pathway identification. We developed a new low density oligonucleotide microarray with 465 probes covering the same number of genes. Target genes were selected to cover most of the well-known biological processes in the stress response documented over the last decade in bivalve species at the cellular and tissue levels. Our new ‘STressREsponse Microarray’ (STREM) platform consists of eight sub-arrays with three replicates for each target in each sub-array. To assess the potential use of the new array, we tested the effect of the ubiquitous environmental pollutant benzo[a]pyrene (B[a]P) at 5, 50, and 100 μg/L on two target tissues, the gills and digestive gland, of Mytilus galloprovincialis exposed invivo for three days. Bioaccumulation of B[a]P was also determined demonstrating exposure in both tissues. In addition to the well-known effects of B[a]P on DNA metabolism and oxidative stress, the new array data provided clues about the implication of other biological processes, such as cytoskeleton, immune response, adhesion to substrate, and mitochondrial activities. Transcriptional data were confirmed using qRT-PCR. We further investigated cellular functions and possible alterations related to biological processes highlighted by the microarray data using oxidative stress biomarkers (Lipofuscin content) and the assessment of genotoxicity. DNA damage, as measured by the alkaline comet assay, increased as a function of dose.DNA adducts measurements using 32P-postlabeling method also showed the presence of bulky DNA adducts (i.e. dG-N2-BPDE). Lipofiscin content increased significantly in B[a]P exposed mussels. Immunohistochemical analysis of tubulin and actin showed changes in cytoskeleton organisation. Our results adopting an integrated approach confirmed that the combination of newly developed transcriptomic approcah, classical biomarkers along with chemical analysis of water and tissue samples should be considered for environmental bioimonitoring and ecotoxicological studies to obtain holistic information to assess the impact of contaminants on the biota.

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“…Excessive exposure to Ni may be harmful to human and animal health [4, 5]. Ni is one of the most commonly detected cutaneous allergens in children in the United States [6].…”

Section: Introductionmentioning

confidence: 99%

Nickel chloride-induced apoptosis via mitochondria- and Fas-mediated caspase-dependent pathways in broiler chickens

et al. 2016

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Ni, a metal with industrial and commercial uses, poses a serious hazard to human and animal health. In the present study, we used flow cytometry, immunohistochemistry and qRT-PCR to investigate the mechanisms of NiCl2-induced apoptosis in kidney cells. After treating 280 broiler chickens with 0, 300, 600 or 900 mg/kg NiCl2 for 42 days, we found that two caspase-dependent pathways were involved in the induced renal tubular cell apoptosis. In the mitochondria-mediated caspase-dependent apoptotic pathway, cyt-c, HtrA2/Omi, Smac/Diablo, apaf-1, PARP, and caspase-9, 3, 6 and 7 were all increased, while. XIAP transcription was decreased. Concurrently, in the Fas-mediated caspase-dependent apoptotic pathway, Fas, FasL, caspase-8, caspase-10 and Bid levels were all increased. These results indicate that dietary NiCl2 at 300+ mg/kg induces renal tubular cell apoptosis in broiler chickens, involving both mitochondrial and Fas-mediated caspase-dependent apoptotic pathways. Our results provide novel insight into Ni and Ni-compound toxicology evaluated in vitro and in vivo.

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Uptake and biochemical responses of mussels Mytilus galloprovincialis exposed to sublethal nickel concentrations

Cited by 62 publications

References 31 publications

Waterborne nickel bioaccumulation in Gammarus pulex: Comparison of mechanistic models and influence of water cationic composition

Waterborne nickel bioaccumulation in Gammarus pulex: Comparison of mechanistic models and influence of water cationic composition

Assessing the impact of Benzo[a]pyrene on Marine Mussels: Application of a novel targeted low density microarray complementing classical biomarker responses

Nickel chloride-induced apoptosis via mitochondria- and Fas-mediated caspase-dependent pathways in broiler chickens

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