Upstream and downstream signals of nitric oxide in pathogen defence

Gaupels, Frank; Kuruthukulangarakoola, Gitto Thomas; Durner, Jörg

doi:10.1016/j.pbi.2011.07.005

Cited by 101 publications

(56 citation statements)

References 57 publications

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“…To recognize their versatility, these have been named moonlighting proteins (Jeffery, 1999;Huberts and van der Klei, 2010;Sirover, 2011) and different mechanisms were proposed for the control of their moonlighting activity, chiefly posttranslational modifications and protein-protein interactions (Moore, 2004). Several reviews, largely inspired by studies on the animal field, have recently proposed plant GAPDH as an example of a moonlighting protein and S-nitrosylation of its catalytic Cys as a possible means to control GAPDH moonlighting activities (Astier et al, 2011;Gaupels et al, 2011). However, despite a general interest in nonglycolytic roles of plant GAPDHs, few reports have experimentally addressed the topic.…”

Section: Discussionmentioning

confidence: 99%

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

et al. 2013

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NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous enzyme involved in the glycolytic pathway. It has been widely demonstrated that mammalian GAPDH, in addition to its role in glycolysis, fulfills alternative functions mainly linked to its susceptibility to oxidative posttranslational modifications. Here, we investigated the responses of Arabidopsis (Arabidopsis thaliana) cytosolic GAPDH isoenzymes GAPC1 and GAPC2 to cadmium-induced stress in seedlings roots. GAPC1 was more responsive to cadmium than GAPC2 at the transcriptional level. In vivo, cadmium treatments induced different concomitant effects, including (1) nitric oxide accumulation, (2) cytosolic oxidation (e.g. oxidation of the redox-sensitive Green fluorescent protein2 probe), (3) activation of the GAPC1 promoter, (4) GAPC1 protein accumulation in enzymatically inactive form, and (5) strong relocalization of GAPC1 to the nucleus. All these effects were detected in the same zone of the root tip. In vitro, GAPC1 was inactivated by either nitric oxide donors or hydrogen peroxide, but no inhibition was directly provided by cadmium. Interestingly, nuclear relocalization of GAPC1 under cadmium-induced oxidative stress was stimulated, rather than inhibited, by mutating into serine the catalytic cysteine of GAPC1 (C155S), excluding an essential role of GAPC1 nitrosylation in the mechanism of nuclear relocalization, as found in mammalian cells. Although the function of GAPC1 in the nucleus is unknown, our results suggest that glycolytic GAPC1, through its high sensitivity to the cellular redox state, may play a role in oxidative stress signaling or protection in plants.

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Section: Discussionmentioning

confidence: 99%

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

et al. 2013

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“…Nitric oxide functions as a signaling molecule and plays an important role during interaction of plant and pathogen (Guo et al 2004;Qiao et al 2015). This gaseous molecule is a signaling messenger involved in plant responses to different stresses (Gaupels et al 2011). Like ROS, NO is an important messenger in many physiological processes and defense reactions in cooperation with ROS (Hong et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

Khaledi¹,

Taheri²,

Falahati-Rastegar³

2018

Journal of Plant Protection Research

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Fusarium graminearum and F. culmorum are the causal agents of Fusarium head blight (FHB) in cereal crops worldwide. Application of resistant cultivars is the most effective and economic method for management of FHB and reducing mycotoxin production in wheat. Understanding the physiological and biochemical mechanisms involved in basal resistance of wheat to FHB disease is limited. In this research, after screening resistance levels of eighteen wheat cultivars planted in Iran, Gaskozhen and Falat were identified as partially resistant and susceptible wheat cultivars against Fusarium spp., respectively. Also, we investigated the role of hydroxyl radical (OH − ), nitric oxide (NO), callose deposition, lipid peroxidation and protein content in basal resistance of wheat to the hemi-biotrophic and necrotrophic Fusarium species causing FHB. Nitric oxide as a signaling molecule may be involved in physiological and defensive processes in plants. Our results showed that NO generation increased in seedlings and spikes of wheat cultivars after inoculation with Fusarium species. We observed earlier and stronger callose deposition at early time points after infection by Fusarium spp. isolates than in non-infected plants, which was positively related to the resistance levels in wheat cultivars. Higher levels of OH − and malondialdehyde (MDA) accumulation (as a marker of lipid peroxidation) were observed in the Falat than in the Gaskozhen cultivar, under non-infected and infected conditions. So, estimation of lipid peroxidation could be useful to evaluate cultivars' susceptibility. These findings can provide novel insights for better recognition of physiological and biochemical markers of FHB resistance, which could be used for rapid screening of resistance levels in wheat cultivars against this destructive fungal disease.

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“…1 The mechanisms by which it exerts its effects are being discovered and include the mobilization of classical second messengers such as Ca 2+ and cyclic GMP, the regulation of protein kinase activities and gene expression, the modulation of lipid signaling and the post-translational modification of numerous proteins. [1][2][3][4][5] During the past 10 y, it has been recognized that NO also acts as a signaling molecule mediating iron deficiency responses. 6 Treatment with artificially-released NO was shown to improve the fitness of maize and tomato plants grown under iron deficiency.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide and glutathione impact the expression of iron uptake- and iron transport-related genes as well as the content of metals inA. thalianaplants grown under iron deficiency

Koen

Szymańska

Klinguer

et al. 2012

Plant Signaling & Behavior

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Upstream and downstream signals of nitric oxide in pathogen defence

Cited by 101 publications

References 57 publications

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

Nitric oxide and glutathione impact the expression of iron uptake- and iron transport-related genes as well as the content of metals inA. thalianaplants grown under iron deficiency

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