Upregulation of vitamin D receptor levels by 1,25(OH) 2 vitamin D 3 in cultured human keratinocytes

Sølvsten, Henrik; Svendsen, Mathias Tiedemann; Fogh, Karsten; Kragballe, Knud

doi:10.1007/s004030050206

Cited by 31 publications

(18 citation statements)

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“…This observation is consistent with previous data reported in the literature, showing that mean intracellular VDR content is higher in undifferentiated than in differentiated cells [35, 38]. Moreover, VDR expression is sustained in myoblasts of different origins as well as in the developing skeletal muscle [21, 39, 40].…”

Section: Discussionsupporting

confidence: 92%

“…Of interest, in the AH130 hosts treated with VitD, muscle VDR levels were higher than those detected in the untreated tumor-bearing rats, likely in view of the restoration of normal circulating vitamin levels. In this regard, it is well known that VitD positively regulates the expression of its main receptor both in vivo and in vitro [34, 35]. The other way round, the present study shows that increased VDR levels in the muscle of tumor-bearing animals seems a constant finding, irrespective of changes in circulating VitD levels.…”

Section: Discussionsupporting

confidence: 74%

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Vitamin D and VDR in cancer cachexia and muscle regeneration

Camperi

Costamagna

et al. 2017

Oncotarget

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Low circulating levels of vitamin D were associated with decreased muscle strength and physical performance. Along this line, the present study was aimed to investigate: i) the therapeutic potential of vitamin D in cancer-induced muscle wasting; ii) the mechanisms by which vitamin D affects muscle phenotype in tumor-bearing animals.Rats bearing the AH130 hepatoma showed decreased circulating vitamin D compared to control rats, while muscle vitamin D receptor (VDR) mRNA was up-regulated. Both circulating vitamin D and muscle VDR expression increased after vitamin D administration, without exerting appreciable effects on body weight and muscle mass.The effects of vitamin D on muscle cells were studied in C2C12 myocytes. Vitamin D-treated myoblasts did not differentiate properly, fusing only partially and forming multinucleated structures with aberrant shape and low myosin heavy chain content. Vitamin D treatment resulted in VDR overexpression and myogenin down-regulation. Silencing VDR expression in C2C12 cultures abrogated the inhibition of differentiation exerted by vitamin D treatment.These data suggest that VDR overexpression in tumor-bearing animals contributes to muscle wasting by impairing muscle regenerative program. In this regard, attention should be paid when considering vitamin D supplementation to patients affected by chronic pathologies where muscle regeneration may be involved.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 74%

Vitamin D and VDR in cancer cachexia and muscle regeneration

Camperi

Costamagna

et al. 2017

Oncotarget

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“…These results are in line with our recent data (Weber et al, 2005) and indicate that the upregulation of hCAP18 mRNA is associated with UVB-induced production of vitamin D. The upregulation of VDR after UVB irradiation is in accordance with previous findings demonstrating that vitamin D induces the expression of VDR in keratinocytes (Solvsten et al, 1997). The regulation of VDR expression, however, seems complex and it has been reported that UVB transiently downregulates the expression of VDR in keratinocytes in vitro up to 24 h following irradiation (Courtois et al, 1998 In humans, skin is the major source for vitamin D and interestingly its production in response to UVB is conserved through evolution.…”

Section: Uvb Upregulates the Antimicrobial Protein Hcap18 Mrna In Humsupporting

confidence: 94%

UVB Upregulates the Antimicrobial Protein hCAP18 mRNA in Human Skin

Mallbris

Edström

Sundblad

et al. 2005

Journal of Investigative Dermatology

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“…2A, 1,25-(OH) 2 D 3 up-regulated CCR10 expression in KAR PAS-620 cells. 1,25-(OH) 2 D 3 also up-regulated VDR expression, as reported previously (43). By real-time PCR, it was determined that 1,25-(OH) 2 D 3 up-regulated the levels of CCR10 mRNA and VDR mRNA in KARPAS-620 cells several folds (Fig.…”

Section: Identification Of the Major Regulatory Elements In The Humansupporting

confidence: 55%

1,25-Dihydroxyvitamin D3 Induces CCR10 Expression in Terminally Differentiating Human B Cells

Shirakawa

Nagakubo

Hieshima

et al. 2008

The Journal of Immunology

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In the B cell lineage, CCR10 is known to be selectively expressed by plasma cells, especially those secreting IgA. In this study, we examined the regulation of CCR10 expression in terminally differentiating human B cells. As reported previously, IL-21 efficiently induced the differentiation of activated human CD19+ B cells into IgD−CD38+ plasma cells in vitro. A minor proportion of the resulting CD19+IgD−CD38+ cells expressed CCR10 at low levels. 1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3), the active metabolite of vitamine D3, dramatically increased the proportion of CD19+IgD−CD38+ cells expressing high levels of CCR10. The 1,25-(OH)2D3 also increased the number of CCR10+ cells expressing surface IgA, although the majority of CCR10+ cells remained negative for surface IgA. Thus, 1,25-(OH)2D3 alone may not be sufficient for the induction of IgA expression in terminally differentiating human B cells. To further determine whether 1,25-(OH)2D3 directly induces CCR10 expression in terminally differentiating B cells, we next performed the analysis on the human CCR10 promoter. We identified a proximal Ets-1 site and an upstream potential vitamin D response element to be critical for the inducible expression of CCR10 by 1,25-(OH)2D3. We confirmed the specific binding of Ets-1 and 1,25-(OH)2D3-activated vitamin D receptor to the respective sites. In conclusion, 1,25-(OH)2D3 efficiently induces CCR10 expression in terminally differentiating human B cells in vitro. Furthermore, the human CCR10 promoter is cooperatively activated by Ets-1 and vitamin D receptor in the presence of 1,25-(OH)2D3.

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Upregulation of vitamin D receptor levels by 1,25(OH) 2 vitamin D 3 in cultured human keratinocytes

Cited by 31 publications

References 0 publications

Vitamin D and VDR in cancer cachexia and muscle regeneration

Vitamin D and VDR in cancer cachexia and muscle regeneration

UVB Upregulates the Antimicrobial Protein hCAP18 mRNA in Human Skin

1,25-Dihydroxyvitamin D3 Induces CCR10 Expression in Terminally Differentiating Human B Cells

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