Upregulation of Talin-1 expression associates with advanced pathological features and predicts lymph node metastases and biochemical recurrence of prostate cancer

Abstract

Talin-1 functions to regulate cell–cell adhesion, and its altered expression was reported to be associated with human carcinogenesis.A total of 280 tissue specimens from prostate cancer (PCa) patients who underwent radical prostatectomy, 75 cases of benign prostatic hyperplasia (BPH) tissue, and 6 cases of normal prostate tissue specimens were collected for construction of tissue microarray and subsequently subjected to immunohistochemical staining of Talin-1 expression.Talin-1 expression was significantly higher in PCa than both normal and BPH tissues (P <0.001). Talin-1 expression in PCa tissues was associated with preoperative prostate-specific antigen (PSA) level, Gleason score, tumor stage, lymph node metastasis, positive surgical margin, extracapsular extension and seminal vesicle invasion (all P <0.05). Logistic regression analysis showed that Talin-1 and Gleason score were independent risk factors for lymph node metastasis of PCa (P <0.001). Receiver operating characteristic (ROC) curve indicated that Talin-1 expression (AUC = 0.766) had a better accuracy to predict PCa lymph node metastasis than Gleason score (AUC = 0.697), whereas their combination could further enhance the prediction accuracy (AUC = 0.803). Kaplan–Meier curve analysis showed that increased Talin-1 expression was associated with shortened biochemical-free survival of PCa patients after radical prostatectomy (P <0.001).These findings suggested that Talin-1 protein was significantly upregulated in PCa tissues compared with that of BPH tissue and Talin-1 expression was an independent predictor for lymph node metastasis and biochemical recurrence of PCa. Further study will investigate the underlying molecular mechanism and the role of Talin-1 in PCa.

ARPC4 promotes bladder cancer cell invasion and is associated with lymph node metastasis

“…9 In brief, tissue biopsy specimens embedded in paraffin blocks were obtained from the Pathology Department of the First Affiliated Hospital of Fujian Medical University and sectioned for hematoxylin and eosin staining. 9 In brief, tissue biopsy specimens embedded in paraffin blocks were obtained from the Pathology Department of the First Affiliated Hospital of Fujian Medical University and sectioned for hematoxylin and eosin staining.…”

“…9 Briefly, TMA sections were de-waxed and rehydrated, and then a rabbit monoclonal anti-ARPC4 antibody was used to incubate with sections at 4°C overnight (1:200 dilution; Abcam, Cambridge, UK). 9 Briefly, TMA sections were de-waxed and rehydrated, and then a rabbit monoclonal anti-ARPC4 antibody was used to incubate with sections at 4°C overnight (1:200 dilution; Abcam, Cambridge, UK).…”

“…9,11,12 Briefly, the staining intensity score was multiplied by the percent positivity score, and this value was then used to derive the final ARPC4 expression score as follows: "-" (score 0-1), "+" (score 2-3), "+ +" (score 4-5), and "++ +" (score ≥ 6). 9,11,12 Briefly, the staining intensity score was multiplied by the percent positivity score, and this value was then used to derive the final ARPC4 expression score as follows: "-" (score 0-1), "+" (score 2-3), "+ +" (score 4-5), and "++ +" (score ≥ 6).…”

“…Immunohistochemistry was performed according to the experimental procedures described in the previous study. 9 Briefly, TMA sections were de-waxed and rehydrated, and then a rabbit monoclonal anti-ARPC4 antibody was used to incubate with sections at 4°C overnight (1:200 dilution; Abcam, Cambridge, UK). After that, sections were washed three times using phosphate buffered saline (PBS) and then secondary antibody (Dako, CA) was used to incubate with washed sections.…”

“…A previously described semi-quantitative method that included staining intensity and the proportion of positive cells was used to derive the total ARPC4 immunostaining score. 9,11,12 Briefly, the staining intensity score was multiplied by the percent positivity score, and this value was then used to derive the final ARPC4 expression score as follows: "-" (score 0-1), "+" (score 2-3), "+ +" (score 4-5), and "++ +" (score ≥ 6).…”