Upregulation of Reg 1α and GW112 in the epithelium of inflamed colonic mucosa

Abstract

Background and aims-Colonic epithelium is involved in the regulation of intestinal function and mucosal immune responses, and its function is altered in inflammatory bowel disease (IBD). However, a comprehensive analysis of the genetic alterations in inflamed colonic epithelium is not available at present. The aim of our study was to detect genes that are preferentially expressed in inflamed colonic epithelia and clarify the biochemical responses of epithelial cells in inflamed colonic mucosa. Methods-cDNA representation diVerence analysis was used to identify candidate genes selectively expressed in inflamed colonic epithelia. Selective expression of these genes in the epithelium of inflamed colonic mucosa, including IBD and non-IBD tissues, was examined by real time polymerase chain reaction and in situ hybridisation. The eVect of cell confluence and inflammatory mediators on Reg 1 gene expression was examined using a colon cancer cell line (HT29). Results-We identified seven candidate genes that were presumed to be upregulated in the inflamed colonic epithelium. Of these, Reg 1 and GW112 were the dominant species and expression of these genes was confined to the crypt epithelium. In vitro studies using a colonic epithelial cell line suggested that cell confluence regulates Reg 1 gene expression. Conclusions-Selective expression of Reg 1 and GW112 genes in the crypt epithelium of inflamed colonic mucosa suggests the important regulatory functions of these genes. (Gut 2001;48:623-629)

Target Genes of Peroxisome Proliferator-activated Receptor γ in Colorectal Cancer Cells

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Olfactomedin 4 down-regulates innate immunity against Helicobacter pylori infection

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Serum regenerating islet‐derived 3‐alpha is a biomarker of mucosal enteropathies

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