Upregulation of NCX Protein in Hepatopancreas and Antennal Gland of Freshwater Crayfish Associated with Elevated CA2+ Flux

Stiner, LaTonia; Gao, Yongping; Wheatly, Michèle G.

doi:10.1007/0-387-23752-6_49

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“…Other studies in our laboratory have used similar techniques to document upregulation (8-18-fold increases) of the primary basolateral Ca 2+ efflux mechanisms [PMCA Wheatly et al, 2004); NCX (Stiner et al, 2004)] during preand postmolt. This would suggest that genes controlling apical Ca 2+ entry (ECaC) and basolateral exit from cells (PMCA, NCX) are closely regulated during periods of elevated transcellular Ca 2+ flux.…”

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confidence: 99%

Molecular characterization of an epithelial Ca2+ channel-like gene from crayfishProcambarus clarkii

Gao

Wheatly

2007

Journal of Experimental Biology

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SUMMARY This study describes the cloning, sequencing and functional characterization of an epithelial Ca2+ channel (ECaC)-like gene isolated from antennal gland (kidney) of the freshwater crayfish Procambarus clarkii. The full-length cDNA consisted of 2687 bp with an open reading frame of 2169 bp encoding a protein of 722 amino acids with a predicted molecular mass of 81.7 kDa. Crayfish ECaC had 76–78% identity at the mRNA level (80–82% amino acid identity) with published fish sequences and 56–62% identity at the mRNA level (52–60% amino acid identity) with mammalian ECaCs. Secondary structure of the crayfish ECaC closely resembled that of cloned ECaCs. Postmolt ECaC expression was exclusively restricted to epithelia associated with Ca2+ influx and was virtually undetectable in non-epithelial tissues (eggs, muscle). Compared with expression levels in hepatopancreas, expression in gill was 10-fold greater and expression was highest in antennal gland (15-fold greater than in hepatopancreas). Compared with baseline expression levels in intermolt stage,expression of ECaC in antennal gland increased 7.4- and 23.8-fold,respectively, in pre- and postmolt stages of the molting cycle. This increase was localized primarily in the labyrinth and nephridial canal, regions of the antennal gland associated with renal Ca2+ reabsorption. The ECaC in crayfish appears to be expressed in epithelia associated with unidirectional Ca2+ influx and relative expression is correlated with rate of Ca2+ influx.

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Section: +mentioning

confidence: 99%