Upregulation of mitochondrial dynamics is responsible for osteogenic differentiation of mesenchymal stem cells cultured on self-mineralized collagen membranes

Update on the effects of energy metabolism in bone marrow mesenchymal stem cells differentiation

“…AMPK inhibitor treatment decreased ATP levels and osteogenesis marker ALP expression in hMSCs while AMPK activators can reverse ATP and ALP levels [ 57 ]. Other recent work has also proved that the HPAA (high-molecular-weight polyacrylic acid)-crosslinked collagen membranes (HCM) can self-mineralize and simulate extracellular matrix (ECM) stiffness, which expedites in situ bone regeneration to guide osteogenic differentiation of BMSCs [ 58 , 59 ]. Regarding the mechanism, HCM promotes mitochondriogenesis and declines mitochondria fission to respond to high energy demand during osteogenic differentiation of BMSCs [ 59 ].…”

“…Other recent work has also proved that the HPAA (high-molecular-weight polyacrylic acid)-crosslinked collagen membranes (HCM) can self-mineralize and simulate extracellular matrix (ECM) stiffness, which expedites in situ bone regeneration to guide osteogenic differentiation of BMSCs [ 58 , 59 ]. Regarding the mechanism, HCM promotes mitochondriogenesis and declines mitochondria fission to respond to high energy demand during osteogenic differentiation of BMSCs [ 59 ]. This in vitro evidence indicates that high ECM stiffness may promote mitochondriogenesis, increase ATP, and decline mitochondria fission to enhance osteogenic differentiation of BMSCs.…”

A novel anti-washout curing solution of calcium phosphate cement prepared via irradiation polymerization

“…The increase of the carboxyl group absorption peak was due to the increase of the sodium polyacrylate concentration in CPC curing solution., mMany studies have shown that sodium polyacrylate with a carboxyl active functional group has a certain ability to promote osteogenic differentiation, Liu et al 56 demonstrated that the carboxyl groups had a positive promoting effect on the proliferation and differentiation of osteoblasts., Su et al 57 investigated the effect of a bioactive LAPONITE® nanosheet (LAP) modified by sodium polyacrylate in the formation of a regenerated silk fibroin (RSF) hydrogel on promoting osteogenic differentiation., tThe result showed that the transcript levels for alkaline phosphatase, osteocalcin, osteopontin, and collagen type I osteogenic markers obviously improve with the RSF/LAP hydrogel compared to the controls at 14 days, especially with higher contents of LAP. Wan et al 58 proposed that the collagen membrane crosslinked with high molecular weight polyacrylic acid hasve been shown to promote osteogenic differentiation of mesenchymal stem cells (MSCs) and enhance bone regeneration in vivo , and polyacrylic acid could guide osteogenic differentiation of MSCs by upregulating mitochondrial dynamics through mitochondriogenesis, fusion, fission and mitophagy. Besides, the porosity of PA-CPCs increased with the rise of sodium polyacrylate concentration in the CPC curing solution, and the higher porosity structure is conducive to the release of carboxyl groups during cell culture, thus stimulating osteoblast differentiation of mBMSCs.…”

Dynamic-stiffening collagen-coated substrate enhances osteogenic differentiation of mesenchymal stem cells through integrin α2β1

“…In addition, the content of collagen I showed a significant effect in promoting the osteogenic differentiation of the rMSCs. 47 The MSCs gradually increase the expression of collagen I during the osteoblast differentiation process, and the accumulated collagen may further accelerate integrin α2β1 activation. In this work, the results indicated that the dynamic-stiffening substrate could accelerate the distribution of integrin α2β1 and promote the differentiation of rMSCs into osteoblasts.…”