Upregulation of miRNA-10a-5p promotes tumor progression in cervical cancer by suppressing UBE2I signaling

Gu, Yannan; Xu, Feng; Jin, Yinzhe; Liu, Yuanlin; Zeng, Li; Zhou, Dachun; Feng, Yuling

doi:10.1080/01443615.2023.2171283

Cited by 7 publications

(4 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most studies related to gene regulation by down-or up-regulated miRNAs were performed in cancer cells [33,36,37,50]. We found that eNK cells use similar mechanisms as cancer cells to proliferate and evade apoptosis.…”

Section: Discussionmentioning

confidence: 84%

See 1 more Smart Citation

Dynamic Changes in miRNA Expression during the Generation of Expanded and Activated NK Cells

Reina-Ortiz,

Mozas,

Ovelleiro

et al. 2023

IJMS

View full text Add to dashboard Cite

Therapies based on allogenic Natural Killer (NK) cells are becoming increasingly relevant, and our laboratory has produced expanded and activated NK (eNK) cells that are highly cytotoxic against several hematological cancers when used alone or in combination with currently approved therapeutic monoclonal antibodies. In order to produce eNK cells, healthy human donor NK cells undergo a 20-day expansion protocol with IL-2, IL-15 and Epstein–Barr virus (EBV)-transformed lymphoblastoid feeder cells. In order to produce an even more potent eNK-based therapy, we must elucidate the changes our protocol produces within healthy NK cells. To understand the post-transcriptional changes responsible for the increased cytolytic abilities of eNK cells, we performed microRNA (miRNA) expression analysis on purified NK cells from day 0 and day 20 of the protocol using quantitative reverse transcription PCR (RT-qPCR). Of the 384 miRNAs profiled, we observed changes in the expression of 64 miRNAs, with especially significant changes in 7 of them. The up-regulated miRNAs of note were miRs-146a, -124, -34a, and -10a, which are key in the regulation of cell survival through the modulation of pro-apoptotic genes such as PUMA. The down-regulation of miRs- 199a, -223, and -340 was also detected and is associated with the promotion of NK cell cytotoxicity. We validated our analysis using immunoblot and flow cytometry studies on specific downstream targets of both up- and down-regulated miRNAs such as PUMA and Granzyme B. These results corroborate the functional importance of the described miRNA expression patterns and show the wide variety of changes that occur in eNK cells at day 20.

show abstract

Section: Discussionmentioning

confidence: 84%

“…miR-10a is significantly up-regulated at day 20 (fc = 38.856; p < 0.01). The up-regulation of miR-10a has been described in several cancer types [49,50]. In acute myeloid leukemia, miR-10a directly binds to several key p53-dependent genes, suppressing p53 central role in cell cycle arrest and apoptosis [51].…”

Section: Up-regulated Differentially Expressed Mirnasmentioning

confidence: 99%

Dynamic Changes in miRNA Expression during the Generation of Expanded and Activated NK Cells

Reina-Ortiz,

Mozas,

Ovelleiro

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Circ_0006789 was originally thought to be able to regulate the growth, migration and invasion of hepatocellular carcinoma cells by modulating the miR-1324/SOX 12 axis [ 13 ], but no studies have yet explored circ_0006789's role in CC. Various miRNAs have been implicated in CC development, either as tumor suppressors or oncogenes[ 14 – 16 ]. Notably, miR-615-5p has been implicated in ovarian cancer [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

circ_0006789 promotes cervical cancer development via the miR-615-5p/HSF1 axis

Zhou,

Song,

2024

Discov Onc

View full text Add to dashboard Cite

Objective Circular RNAs (circRNAs) are involved in the development of human cancers, including cervical cancer (CC). However, the role and mechanism of circ_0006789 (circSLC25A43) in CC are unclear. The purpose of this study was to investigate the functional role of circ_0006789 in CC. Methods The expression of circ_0006789 in CC tissues and cell lines was examined by RT-qPCR. The characterization of circ_0006789 in CC cells was verified by subcellular localisation, actinomycin D assay, and RNase R assay. After circ_0006789 was knocked down in CC cell lines, the proliferation, apoptosis, migration and invasion of CC cells were assessed by CCK-8 method, flow cytometry, and Transwell assay. RIP assay, FISH assay, dual luciferase reporter gene assay and Western blot were used to investigate the regulatory mechanism between circ_0006789, miR-615-5p and heat shock factor 1 (HSF1). Results circ_0006789 was upregulated in CC tissues and cell lines. CC cells were inhibited in their proliferation, migration, and invasion, as well as promoted to apoptosis when circ_0006789 was knocked down. It was found that circ_0006789 targeted miR-615-5p, and miR-615-5p expression was inversely correlated with circ_0006789 expression. Furthermore, HSF1 was a target gene of miR-615-5p. Furthermore, the suppressive effects on HeLa cells mediated by circ_0006789 knockdown were counter-balanced when miR-615-5p was knocked down and HSF1 was overexpressed. Mechanistically, circ_0006789 was found to promote CC development by reducing miR-615-5p and increasing HSF1 expressions. Conclusion circ_0006789 accelerates CC development via the miR-615-5p/HSF1 axis.

show abstract

“…However, the most apparent cause is the infection with human papillomavirus (HPV) [ 6 , 7 ]. The current primary treatment for cervical cancer contains radiotherapy, surgical resection, and locally targeted therapy [ 8 , 9 ]. These therapies are only effective in cases of early-stage cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

LncRNA ABHD11-AS1 activates EGFR signaling to promote cervical cancer progression by preventing FUS-mediated degradation of ABHD11 mRNA

Yang,

Tian,

Zhao

et al. 2023

Cell Cycle

View full text Add to dashboard Cite

Cervical cancer is one of the most common gynecological cancers with high metastasis, poor prognosis and conventional chemotherapy. The long non-coding RNA (lncRNA) ABHD11 antisense RNA 1 (ABHD11-AS1) plays a vital role in tumorigenesis and is involved in cell proliferation, differentiation, and apoptosis. Especially for cervical cancer, the functions and mechanisms of ABHD11-AS1 are still undetermined. In this study, we explored the role and underlying mechanism of ABHD11-AS1 in cervical cancer. We found that ABHD11-AS1 is highly expressed in cervical cancer tissue. The roles of ABHD11-AS1 and EGFR have investigated the loss of function analysis and cell movability in SiHa and Hela cells. Knockdown of ABHD11-AS1 and EGFR significantly inhibited the proliferation, migration, and invasion and promoted apoptosis of SiHa and Hela cells by up-regulating p21 and Bax and down-regulating cyclin D1, Bcl2, MMP9, and Vimentin. ABHD11-AS1 knockdown could decrease the expression of EGFR. In addition, ABHD11-AS1 could regulate the EGFR signaling pathway, including p-EGFR, p-AKT, and p-ERK. Spearman’s correlation analysis and cell experiments demonstrated that ABHD11 was highly expressed in tumor tissue and partially offset the effect of shABHD11-AS1 on the proliferation, migration, and invasion of SiHa and Hela cells. Then, RNA pulldown was used to ascertain the mechanisms of ABHD11-AS1 and FUS. ABHD11-AS1 inhibited ABHD11 mRNA degradation by bounding to FUS. A subcutaneous xenograft of SiHa cells was established to investigate the effect of ABHD11-AS1 in tumor tissue. Knockdown of ABDH11-AS1 inhibited tumor growth and decreased the tumor volume. ABHD11-AS1 knockdown inhibited the expression of Ki67 and Vimentin and up-regulated the expression of Tunel. Our data indicated that ABHD11-AS1 promoted cervical cancer progression by activating EGFR signaling, preventing FUS-mediated degradation of ABHD11 mRNA. Our findings provide novel insights into the potential role of lncRNA in cervical cancer therapy.

show abstract

Upregulation of miRNA-10a-5p promotes tumor progression in cervical cancer by suppressing UBE2I signaling

Cited by 7 publications

References 41 publications

Dynamic Changes in miRNA Expression during the Generation of Expanded and Activated NK Cells

Dynamic Changes in miRNA Expression during the Generation of Expanded and Activated NK Cells

circ_0006789 promotes cervical cancer development via the miR-615-5p/HSF1 axis

LncRNA ABHD11-AS1 activates EGFR signaling to promote cervical cancer progression by preventing FUS-mediated degradation of ABHD11 mRNA

Contact Info

Product

Resources

About