Upregulation of miR-483-3p contributes to endothelial progenitor cells dysfunction in deep vein thrombosis patients via SRF

J Cellular Molecular Medi

Lei

et al. 2018

116

MicroRNAs (miRNAs) are small non‐coding RNAs that regulate gene expression at a post‐transcriptional level via either the degradation or translational repression of a target mRNA. They play an irreplaceable role in angiogenesis by regulating the proliferation, differentiation, apoptosis, migration and tube formation of angiogenesis‐related cells, which are indispensable for multitudinous physiological and pathological processes, especially for the occurrence and development of vascular diseases. Imbalance between the regulation of miRNAs and angiogenesis may cause many diseases such as cancer, cardiovascular disease, aneurysm, Kawasaki disease, aortic dissection, phlebothrombosis and diabetic microvascular complication. Therefore, it is important to explore the essential role of miRNAs in angiogenesis, which might help to uncover new and effective therapeutic strategies for vascular diseases. This review focuses on the interactions between miRNAs and angiogenesis, and miRNA‐based biomarkers in the diagnosis, treatment and prognosis of angiogenesis‐related diseases, providing an update on the understanding of the clinical value of miRNAs in targeting angiogenesis.

Section: Mir-483mentioning

confidence: 88%

The regulatory role of microRNAs in angiogenesis‐related diseases

J Cellular Molecular Medi

Lei

et al. 2018

116

“…In recent years, multiple miRNAs have been reported to play a vital role in angiogenesis, cell growth and migration . Our previous studies have demonstrated that miRNAs have a vital role in EPC function . Additionally, miR‐205 has been shown to have different effects on angiogenesis and migration in different types of tumours .…”

Section: Discussionmentioning

confidence: 99%

“…26 Our previous studies have demonstrated that miRNAs have a vital role in EPC function. 11,[27][28][29][30] Additionally, miR-205 has been shown to have different effects on angiogenesis and migration in different types of tumours. [32][33][34][35]42 Meanwhile, miR-205 promotes wound healing in human corneal epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…They play a major role in the regulation of cell migration, proliferation, apoptosis and angiogenesis, which is essential for the development and progression of vascular disease . Our previous studies have suggested that miRNAs including miR‐483‐3p, miR‐150, let‐7e‐5p and miR‐126 play a crucial role in regulating angiogenesis and migration in EPCs . A recent study has also indicated that miR‐126‐loaded EPC‐derived exosomes are a promising potential vehicle for DVT therapy .…”

Section: Introductionmentioning

confidence: 99%

“…26 Our previous studies have suggested that miR-NAs including miR-483-3p, miR-150, let-7e-5p and miR-126 play a crucial role in regulating angiogenesis and migration in EPCs. [27][28][29][30] A recent study has also indicated that miR-126-loaded EPC-derived exosomes are a promising potential vehicle for DVT therapy. 11 Among the emerging miRNAs, miR-205 has an important impact on the progression of diseases, but it is a "double-edged sword" in diseases.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

MiR‐205 promotes endothelial progenitor cell angiogenesis and deep vein thrombosis recanalization and resolution by targeting PTEN to regulate Akt/autophagy pathway and MMP2 expression

J Cellular Molecular Medi

Xiao

et al. 2019

MicroRNAs (MiRNAs, MiRs) represent a class of conserved small non‐coding RNAs that affect post‐transcriptional gene regulation and play a vital role in angiogenesis, proliferation, apoptosis, migration and invasion. They are essential for a wide range of physiological and pathological processes, especially for vascular diseases. However, data concerning miRNAs in endothelial progenitor cells (EPCs) and deep vein thrombosis (DVT) remain incomplete. We explored miRNAs that modulate angiogenesis in EPCs and thrombolysis, and analysed their underlying mechanisms using a DVT model, dual‐luciferase reporter assay, qRT‐PCR, Western blot, immunofluorescence staining, flow cytometry analysis, CCK‐8 assay, angiogenesis assay, wound healing and Transwell assay. We found that miR‐205 enhanced the homing ability of EPCs to DVT sites and promoted thrombosis resolution and recanalization, which significantly reduced venous thrombus. Additionally, we demonstrated that miR‐205 overexpression significantly enhanced angiogenesis in vivo and in vitro, migration, invasion, F‐actin filaments and proliferation in EPCs, and inhibited cell apoptosis. Conversely, down‐regulation of miR‐205 played the opposite role in EPCs. Importantly, this study demonstrated that miR‐205 directly targeted PTEN to modulate the Akt/autophagy pathway and MMP2 expression, subsequently playing a key role in EPC function and DVT recanalization and resolution. These results elucidated the pro‐angiogenesis effects of miR‐205 in EPCs and established it as a potential target for DVT treatment.

Retracted: Evaluation of the circulating MicroRNA‐495 and Stat3 as prognostic and predictive biomarkers for lower extremity deep venous thrombosis

Nai-xuan

J of Cellular Biochemistry

2018

This study aims to elucidate the prognostic and predictive biomarker of miR-495 and Stat3 in peripheral blood in relation to lower extremity deep venous thrombosis (DVT). Patients with lower limb fractures were assigned into case and control groups. Rats were allocated into blank (normal rats), sham (normal rats), DVT, miR-495 mimic, miR-495 inhibitor, over-Stat3, and si-Stat3 groups. ELISA was used to detect levels of prothrombin time (PT), endothelin-1 (ET-1), Human Fibrinogen (FIB), D-Dimer, blood coagulation factors V and VIII, tissue type plasminogen activator (t-PA), platelet activating factor (PAF), protein C and Stat3. qRT-PCR was employed for the evaluation of the expressions of miR-495 and Stat3, while receiver operating characteristic (ROC) curve was constructed to assess the predictive value of miR-495 and Stat3 as well as the treatment outcomes of patients with lower limb fractures. Logistic regression analyses were conducted in order to correlate indexes and lower extremity DVT. miR-495 overexpression, t-PA, PAF, and protein C were confirmed to be protective factors, while Stat3 overexpression, PT, ET-1, FIB, D-Dimer, blood coagulation factor V, and VIII were all ultimately considered to be risk factors of lower extremity DVT. Stat3 was confirmed to be the target gene of miR-495. Compared with the blank group, the length and weight of the thrombus as well as the ratio between length and weight, mRNA and protein expression of Stat3 were reduced in the miR-495 mimic and si-Stat3 groups. Our findings suggest that through the suppression of Stat3 expression, miR-495 prohibits lower extremity DVT in peripheral blood.