Upregulation of lipogenic enzymes genes expression in white adipose tissue of rats with chronic renal failure is associated with higher level of sterol regulatory element binding protein-1

Korczyńska, Justyna; Stelmańska, Ewa; Nogalska, Anna; Szołkiewicz, Marek; Goyke, Elżbieta; Świerczyński, Julian; Rutkowski, Bolesław

doi:10.1016/j.metabol.2004.02.015

Cited by 30 publications

(22 citation statements)

References 38 publications

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“…Moreover, in patients with type 2 diabetes/IGT, we found a significant correlation between microalbuminuria and adipose tissue FASN mRNA expression. These results confirm previous studies in rodents, showing that FASN gene expression is upregulated in experimental chronic renal failure [23,32]. However, patients with renal dysfunction were excluded from our study.…”

Section: Resultssupporting

confidence: 92%

Fatty acid synthase gene expression in human adipose tissue: association with obesity and type 2 diabetes

et al. 2007

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Aims/hypothesis Increased expression and activity of the lipogenic pathways in adipose tissue may contribute to the development of obesity. As a central enzyme in lipogenesis, the gene encoding fatty acid synthase (FASN) was identified as a candidate gene for determining body fat. In the present study we tested the hypothesis that increased FASN expression links metabolic alterations of excess energy intake, including hyperinsulinaemia, dyslipidaemia and altered adipokine profile to increased body fat mass. Subjects and methods In paired samples of visceral and subcutaneous adipose tissue from 196 participants (lean or obese), we investigated whether FASN mRNA expression (assessed by PCR) in adipose tissue is increased in obesity and related to visceral fat accumulation, measures of insulin sensitivity (euglycaemic-hyperinsulinaemic clamp) and glucose metabolism.Results FASN mRNA expression was increased by 1.7-fold in visceral vs subcutaneous fat. Visceral adipose tissue FASN expression was correlated with FASN protein levels, subcutaneous FASN expression, visceral fat area, fasting plasma insulin, serum concentrations of IL-6, leptin and retinol-binding protein 4 (RBP4), and inversely with measures of insulin sensitivity, independently of age, sex and BMI. Moreover, we found significant correlations between FASN expression and markers of renal function, including serum creatinine and urinary albumin excretion. Conclusions/interpretation Increased FASN gene expression in adipose tissue is linked to visceral fat accumulation, impaired insulin sensitivity, increased circulating fasting insulin, IL-6, leptin and RBP4, suggesting an important role of lipogenic pathways in the causal relationship between consequences of excess energy intake and the development of obesity and type 2 diabetes.

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Section: Resultssupporting

confidence: 92%

Fatty acid synthase gene expression in human adipose tissue: association with obesity and type 2 diabetes

et al. 2007

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“…The decreased expression of Pparg also suggests a reduction in adipocyte differentiation. The decreased Srebf1 expression in leptin-treated mice is consistent with findings that show that Srebf1 regulates several key genes related to lipid metabolism, including Fas, (49) and is associated with a reduction in lipogenic activity. (50) TNF-a is a cytokine that stimulates lipolysis and induces apoptosis.…”

Section: Discussionsupporting

confidence: 89%

Central (ICV) leptin injection increases bone formation, bone mineral density, muscle mass, serum IGF-1, and the expression of osteogenic genes in leptin-deficient ob/ob mice

Bartell

Rayalam

Ambati

et al. 2011

Journal of Bone and Mineral Research

138

117

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Both central and peripheral leptin administrations reduce body weight, food intake, and adiposity in ob/ob mice. In this study we compared effects of intracerebroventricular (ICV) and subcutaneous (SC) administration of leptin on bone metabolism in the appendicular and axial skeleton and adipose tissue gene expression and determined the effects of ICV leptin on bone marrow gene expression in ob/ob mice. In experiment 1, leptin (1.5 or 0.38 mg/d) or control was continuously injected ICV for 12 days. Gene expression analysis of femoral bone marrow stromal cells showed that expression of genes associated with osteogenesis was increased after ICV injection, whereas those associated with osteoclastogenesis, adipogenesis, and adipocyte lipid storage were decreased. In experiment 2, leptin was injected continuously ICV (0.0 or 1.5 mg/d) or SC (0.0 or 10 mg/d) for 12 days. In both experiments, regardless of mode of administration, leptin decreased body weight, food intake, and body fat and increased muscle mass, bone mineral density, bone mineral content, bone area, marrow adipocyte number, and mineral apposition rate. Serum insulin was decreased, whereas serum osteocalcin, insulin-like growth factor 1, osteoprotegerin, pyridinoline, and receptor activator of nuclear factor kB ligand concentrations were increased. In experiment 2, expression of genes in adipose tissue associated with apoptosis, lipid mobilization, insulin sensitivity, and thermogenesis was increased, whereas expression of genes associated with cell differentiation and maturation was decreased regardless of mode of administration. Thus ICV injection of leptin promotes expression of pro-osteogenic factors in bone marrow, leading to enhanced bone formation in ob/ob mice. ß

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“…Sterol regulatory element binding factor 1 (SREBP-1) has been shown to regulate several key genes related to lipid metabolism, including FAS in the liver (Shimano et al, 1999), cultured adipocytes (Kim et al, 1998;Kim and Spiegelman, 1996) and adipose tissue (Korczynska et al, 2004). Some interactions between SREBP1 and leptin have been previously observed.…”

Section: Discussionmentioning

confidence: 99%

Adipose tissue gene expression profiles in ob/ob mice treated with leptin

et al. 2008

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Upregulation of lipogenic enzymes genes expression in white adipose tissue of rats with chronic renal failure is associated with higher level of sterol regulatory element binding protein-1

Cited by 30 publications

References 38 publications

Fatty acid synthase gene expression in human adipose tissue: association with obesity and type 2 diabetes

Fatty acid synthase gene expression in human adipose tissue: association with obesity and type 2 diabetes

Central (ICV) leptin injection increases bone formation, bone mineral density, muscle mass, serum IGF-1, and the expression of osteogenic genes in leptin-deficient ob/ob mice

Adipose tissue gene expression profiles in ob/ob mice treated with leptin

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