Upregulation of LGALS1 is associated with oral cancer metastasis

Li, Jimin; Tseng, Chung‐Shi; Lin, Chi-Chen; Law, Ching-Hsuan; Chien, Yu‐An; Kuo, Wen‐Hung; Chou, Hsiu‐Chuan; Wang, Wen-Ching; Chan, Hong‐Lin

doi:10.1177/1758835918794622

Cited by 32 publications

(33 citation statements)

References 53 publications

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“…TSPAN15 is believed to enhance tumor stemness, and it has the ability to promote tumor growth and recurrence [30]. TSPAN12 can promote tumor cell proliferation and colony formation, and high expression of CD9 is considered to be related to lymph node metastasis [31,32]. In contrast, another portion of the four-transmembrane protein family showed tumor suppression.…”

Section: Discussionmentioning

confidence: 99%

Expression and function of transmembrane 4 superfamily proteins in digestive system cancers

et al. 2020

Cancer Cell Int

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Background: Although the medical level is constantly improving, cancer is still a major disease that threatens human health, and very effective treatments have not been found. In recent years, studies have found that four-transmembrane superfamily proteins are involved in multiple stages of tumorigenesis and development, but their expression and function in tumors have not been systematically studied. Methods: We used the Oncomine database to analyze the mRNA expression levels of TSPAN family in various cancers. Then differentially expressed genes were screened out and verified by liver cancer, colorectal cancer, and gastric cancer cells by q-PCR and Western blot analysis. CCK8 and EDU analysis are used to detect cell proliferation, Cell wound scrape assay and Cell invasion assay are used to analyze cell invasion and metastasis. Nude tumor formation test used to verify the tumor suppressive effect of TSPAN7 in vivo. Results: Differential analysis of 33 TSPAN proteins revealed that a total of 11 proteins showed differential expression in 10% of independent analyses, namely TSPAN1, TSPAN3, TSPAN5, TSPAN6, TSPAN7, TSPAN8, TSPAN13, TSPAN25, TSPAN26, TSPAN29, TSPAN30. TSPAN7 is the only four-transmembrane protein with reduced expression in three types of digestive tract tumors, so we chose TSPAN7 to be selected for cellular and molecular level verification. We found that compared with normal cells, the expression of TSPAN7 in liver cancer cells was significantly reduced, while the expression of gastric and colon cancer was not significantly different from that of normal cells. In addition, we also found that the high expression of Tspan7 not only inhibited the proliferation of HCC-LM3 cells, but also inhibited its invasion and metastasis. Conclusions: Our study evaluated the expression and function of the TSPANs family in digestive cancers and explored TSPAN7 in hepatoma cells in detail. We found some members of the TSPAN family show significant expression differences between cancer and normal tissues, of which TSPAN7 may be a potential biomarker for liver cancer.

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Section: Discussionmentioning

confidence: 99%

Expression and function of transmembrane 4 superfamily proteins in digestive system cancers

et al. 2020

Cancer Cell Int

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“…After the membranes were blocked with 5% (w/v) skimmed milk or BSA (bovine serum albumin) in Tris-buffered saline with Tween-20 [TBST; 50 mM Tris, 150 mM NaCl, and 0.1% Tween-20 (v/v); pH 8.0] for 1 h, the primary antibodies (Genetex Inc., Hsinchu, Taiwan, Table 1) were diluted 1:2000 and added onto the membranes, which were incubated overnight to 48 h at 4 • C. Following this, the membranes were washed in TBST for 6 times (10 min/wash) and then incubated in TBST solution containing appropriate horseradish peroxidase-coupled secondary antibodies (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) in dilution of 1:10,000 for 1 h with gentle agitation. Then, the membranes were washed again in TBST 6 times (10 min/time), and the probed proteins were visualized using an enhanced chemiluminescence (ECL) method (Visual Protein Biotech Corp., Taipei, Taiwan) [42]. The data of densitometric quantification based on ratio to housekeeping protein (LDH) were evaluated by Image J software.…”

Section: Immunoblotting Analysis Via Western Blotmentioning

confidence: 99%

The Role of Transforming Growth Factor-Beta in Retinal Ganglion Cells with Hyperglycemia and Oxidative Stress

Chen

Chou

et al. 2020

IJMS

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A characteristic of diabetes mellitus is hyperglycemia, which is considered with an emphasis on the diabetic retinopathy of progressive neurodegenerative disease. Retinal ganglion cells (RGCs) are believed to be important cells affected in the pathogenesis of diabetic retinopathy. Transforming growth factor-beta (TGF-β) is a neuroprotective protein that helps to withstand various neuronal injuries. To investigate the potential roles and regulatory mechanisms of TGF-β in hyperglycemia-triggered damage of RGCs in vitro, we established RGCs in 5.5, 25, 50, and 100 mM D-glucose supplemented media and focused on the TGF-β-related oxidative stress pathway in combination with hydrogen peroxide (H2O2). Functional experiments showed that TGF-β1/2 protein expression was upregulated in RGCs with hyperglycemia. The knockdown of TGF-β enhanced the accumulation of reactive oxygen species (ROS), inhibited the cell proliferation rate, and reduced glutathione content in hyperglycemia. Furthermore, the results showed that the TGF-β-mediated enhancement of antioxidant signaling was correlated with the activation of stress response proteins and the antioxidant pathway, such as aldehyde dehydrogenase 3A1 (ALDH3A1), heme oxygenase-1 (HO-1), nuclear factor erythroid 2-related factor (Nrf2), and hypoxia-inducible factor (HIF-1α). Summarizing, our results demonstrated that TGF-β keeps RGCs from hyperglycemia-triggered harm by promoting the activation of the antioxidant pathway, suggesting a potential anti-diabetic therapy for the treatment of diabetic retinopathy.

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“…TSPAN15 is believed to enhance tumor stemness, and it has the ability to promote tumor growth and recurrence [20] . TSPAN12 can promote tumor cell proliferation and colony formation, and high expression of CD9 is considered to be related to lymph node metastasis [21,22] . In contrast, another portion of the four-transmembrane protein family showed tumor suppression.…”

Section: Discussionmentioning

confidence: 99%

Expression and function of transmembrane 4 superfamily proteins in digestive system cancers

et al. 2020

Preprint

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Background Although the medical level is constantly improving, cancer is still a major disease that threatens human health, and very effective treatments have not been found. In recent years, studies have found that four-transmembrane superfamily proteins are involved in multiple stages of tumorigenesis and development, but their expression and prognosis in tumors have not been systematically studied. Methods We used the Oncomine database to analyze the mRNA expression levels of TSPAN family in various cancers. Then differentially expressed genes were screened out and verified by liver cancer, colorectal cancer, and gastric cancer cells by q-PCR and Western blot analysis. CCK8 and EDU analysis are used to detect cell proliferation, Cell wound scrape assay and Cell invasion assay are used to analyze cell invasion and metastasis. Nude tumor formation test used to verify the tumor suppressive effect of TSPAN7 in vivo. Results: Differential analysis of 33 TSPAN proteins revealed that a total of 11 proteins showed differential expression in 10% of independent analyses, namely TSPAN1, TSPAN3, TSPAN5, TSPAN6, TSPAN7, TSPAN8, TSPAN13, TSPAN25, TSPAN26, TSPAN29, TSPAN30 . TSPAN7 is the only four-transmembrane protein with reduced expression in three types of digestive tract tumors, so we chose TSPAN7 to be selected for cellular and molecular level verification. We found that compared with normal cells, the expression of TSPAN7 in liver cancer cells was significantly reduced, while the expression of gastric and colon cancer was not significantly different from that of normal cells. In addition, we also found that the high expression of Tspan7 not only inhibited the proliferation of HCC-LM3 cells, but also inhibited its invasion and metastasis. Conclusions Some members of the TSPAN family show significant expression differences between cancer and normal tissues, of which TSPAN7 may be a potential biomarker for liver cancer.

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Upregulation of LGALS1 is associated with oral cancer metastasis

Cited by 32 publications

References 53 publications

Expression and function of transmembrane 4 superfamily proteins in digestive system cancers

Expression and function of transmembrane 4 superfamily proteins in digestive system cancers

The Role of Transforming Growth Factor-Beta in Retinal Ganglion Cells with Hyperglycemia and Oxidative Stress

Expression and function of transmembrane 4 superfamily proteins in digestive system cancers

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