Upregulation of IBSP Expression Predicts Poor Prognosis in Patients With Esophageal Squamous Cell Carcinoma

Abstract

Esophageal squamous cell carcinoma (ESCC), which is characterized by invasiveness and poor prognosis, is the sixth most common leading cause of cancer-related death worldwide. Despite advances in multimodality therapy, ESCC mortality remains high, and an understanding of the molecular changes that lead to ESCC development and progression remains limited. In the present study, Integrin Binding Sialoprotein (IBSP) upregulation was found in 182 of 269 (67.7%) primary ESCC cells at the mRNA level by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, IHC staining further demonstrated that IBSP was upregulated in ESCC patients and IBSP protein upregulation was significantly related to the lymph node metastasis (P = 0.017), clinicopathologic stage (P = 0.001) and poor disease survival (P = 0.002). Moreover, functional studies illustrated that the IBSP gene can promote the proliferation and metastasis of ESCC cells. Furthermore, IBSP was found to regulate epithelial-mesenchymal transition (EMT), which promotes tumor cell metastasis. In conclusion, our study suggests that IBSP may be a valuable prognostic marker for ESCC patients.

IBSP, a potential recurrence biomarker, promotes the progression of colorectal cancer via Fyn/β‐catenin signaling pathway

“…[24][25][26] A majority of SIBLING family members can be primarily detected within bone tissues, a small portion of these proteins show abnormal expression within malignant cancer tissues. 18,27 The aberrantly expressed IBSP gene is tightly associated with an increased risk of malignant transformation, bone metastasis, along with dismal prognostic outcome of non-small cell lung cancer (NSCLC), BC, and PC. 15,17,[28][29][30] There are few reports on the relationship between IBSP and CRC, only a study of regulation of osteopontin and related proteins in rat CC531 CRC cells.…”

“…IBSP has been recognized to be the possible oncogene, which is usually overexpressed and upregulated in numerous cancers, such as prostate cancer (PC), breast cancer (BC), and lung cancer (LC). [17][18][19][20] Only few studies have reported the relationship between IBSP and CRC, and the biological function and molecular mechanism of IBSP involved in CRC progression are completely unknown. The present work measured IBSP expression within CRC samples at mRNA and protein levels through qRT-PCR together with Western blotting analysis.…”

Tumor Nonimmune-Microenvironment-Related Gene Expression Signature Predicts Brain Metastasis in Lung Adenocarcinoma Patients after Surgery: A Machine Learning Approach Using Gene Expression Profiling

“…COL5A1 is associated with the metastasis of LUAD [86]. IBSP overexpression is significantly related to lymph node metastasis in esophageal squamous cell carcinoma [87]. A high expression of COL5A2 is associated with metastasis in renal cell carcinoma [88].…”

Biomarker Screening and Prognostic Significance Analysis for Renal Cell Carcinoma

“… 40 IBSP encodes a specific regulator of matrix metalloproteinase, and high expression of IBSP is correlated with poor prognosis of esophageal carcinoma. 41 However, the role and expression levels of STEAP3 or IBSP have not been explored in renal cell carcinoma. The current study conducted bioinformatics analysis, and the findings showed that STEAP3 and IBSP are highly expressed in advanced renal cell carcinoma and are implicated in EMT, cell cycle regulation, and angiogenesis.…”