Upregulation of <i>Lnc<scp>RNA</scp>‐<scp>HIT</scp></i> promotes migration and invasion of non‐small cell lung cancer cells by association with <scp>ZEB</scp>1

Jia, Xiaojing; Wang, Zhicheng; Qiu, Ling; Yang, Yanming; Wang, Yunlong; Chen, Zhishen; Liu, Zhongshan; Yu, Lei

doi:10.1002/cam4.948

Cited by 44 publications

(30 citation statements)

References 29 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LncRNAs are known to be involved in multiple key cellular processes; including genomic imprinting, RNA processing, chromatin modification and the modulation of apoptosis and invasion [18][19][20][21]. Emerging evidence shows that lncRNAs are essential for the development of malignant lung tumor carcinogenesis, and these have emerged as biomarkers for tumor prediction and diagnosis [7,22,23]. For example: (1) lncRNA AGAP2-AS1 can promote the NSCLC…”

Section: Discussionmentioning

confidence: 99%

“…growth by interacting with EZH2 and LSD1 and repressing LATS2 and KLF2 expression [24]; (2) LncRNA TATDN1 promotes NSCLC growth and metastasis by suppressing E-cadherin and Ezrin [25]; (3) lncRNA HIT functions as a pro-metastatic oncogene by directly associating with ZEB1 to regulate NSCLC migration, invasion and metastasis [23] and (4) ANRIL [7], PVT1 [22] and SNHG7 [26], PCAT1 [27] act as oncogenes in lung cancer progression.…”

Section: Figure 6 Mechanisms Of Picart1 Exert Its Functions In Lung mentioning

confidence: 99%

See 1 more Smart Citation

Long non-coding RNA PICART1 suppresses proliferation and promotes apoptosis in lung cancer cells by inhibiting JAK2/STAT3 signaling

Zhao

Cheng

et al. 2018

neo

View full text Add to dashboard Cite

Lung cancer remains the most common cause of tumor-related death worldwide. Recent studies have revealed that long non-coding RNAs (lncRNAs) are involved in the development of various cancers, including lung cancer. This study aimed to investigate the effect and the molecular basis of lncRNA PICART1 on lung cancer. We first assessed the PICART1 expression in lung cancer in vitro and vivo by qRT-PCR. Then the expression of PICART1 in SPC-A-1 and NCI-H1975 cell lines was inhibited and overexpressed by transient transfections. Thereafter, cell viability, cell cycle, migration and apoptosis were respectively measured by MTT, Transwell and flow cytometry assay. Furthermore, qRT-PCR and western blot analysis were mainly performed to assess the expression levels of apoptosis- and migration-related proteins and JAK2/STAT3 pathway proteins. Tumor formation was measured by xenograft tumor model assay in vivo. PICART1 expression was down-regulated in human lung cancer tissues and cell lines. Knockdown of PICART1 increased cell viability of lung cancer cell lines. However, PICART1 overexpression inhibited cell cycle progression and promoted apoptosis in SPC-A-1 and NCI-H1975 cell lines. PICART1 overexpression also inhibited migration, as evidenced by up-regulation of E-cadherin, and down-regulation of Twist1, MMP2 and MMP9. Furthermore, we found PICART1 inhibition may regulate cell apoptosis and migration through activating JAK2/STAT3 pathway. In vivo experiments revealed that PICART1 knockdown significantly promoted tumor formation. This study demonstrates that PICART1 overexpression represents an anti-growth and anti-metastasis role in lung cancer cells. Additionally, PICART1 acts as a tumor suppressor may be via regulation of JAK2/STAT3 pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Figure 6 Mechanisms Of Picart1 Exert Its Functions In Lung mentioning

confidence: 99%

Long non-coding RNA PICART1 suppresses proliferation and promotes apoptosis in lung cancer cells by inhibiting JAK2/STAT3 signaling

Zhao

Cheng

et al. 2018

neo

View full text Add to dashboard Cite

show abstract

“…Upregulated lncRNA SNHG1 can contribute to lung cancer development through sponging miR‐101‐3p and activating Wnt signaling . LncRNA‐HIT promotes NSCLC progression by regulating ZEB1 …”

Section: Introductionmentioning

confidence: 99%

LINC‐PINT alleviates lung cancer progression via sponging miR‐543 and inducing PTEN

et al. 2020

View full text Add to dashboard Cite

Long intergenic nonprotein coding RNA p53‐induced transcript (LINC‐PINT) has been reported to participate in various cancers. Here, we investigated the effects of LINC‐PINT on lung cancer progression. Firstly, in our study, we implied that LINC‐PINT was obviously decreased in NSCLC. Thereafter, in A549 and H1299 cells, LINC‐PINT was upregulated via transfecting LV‐LINC‐PINT. As exhibited, LINC‐PINT repressed cell proliferation and cell colony formation of A549 and H1299 cells. Subsequently, flow cytometry evidenced that A549 and H1299 cell apoptosis was obviously triggered and the cell cycle was arrested in G1 phase. Then, migration and transwell invasion experiments were carried out to detect the cell migration and invasion capacity. We found A549 and H1299 cell migration and invasion capacity were restrained by the upregulation of LINC‐PINT. Meanwhile, we predicted that miR‐543 could function as the target of LINC‐PINT and the association was verified. Moreover, we exhibited that miR‐543 was remarkably increased in lung cancer, which could be regulated by LINC‐PINT negatively. Furthermore, PTEN could act as the downstream target of miR‐543 and upregulation of miR‐543 repressed PTEN, which was reversed by LV‐PINT in A549 and H1299 cells. Finally, xenografts were utilized to confirm the function of LINC‐PINT on lung cancer. All these findings concluded that LINC‐PINT exerted crucial biological roles in NSCLC through sponging miR‐543 and inducing PTEN.

show abstract

“…The dysregulated expression of lncRNAs has been proved to promote a variety of tumors development and progression. In NSCLC, over-expression of lncRNA-HIT promotes cancer cells migration and invasion by association with ZEB1 (Jia, et al, 2016); upregulated lncRNA SNHG1 contributes to the cancer progression by inhibition of miR-101-3p and activation of WNT/β-catenin signaling pathway (Liu); lncRNA MALAT1 contributes to the cancer development through regulating miR-206 and miR-124 (Deng, 2018, Hu) and lncRNA UCA1 evokes nonT790M acquired resistance to EGFR-TKIs via activation of AKT/mTOR pathway (Cheng, et al, 2015). Additionally, lncRNAs were also reported to be a potential biomarker in NSCLC widely (Damjan, Osielska and Jagodziński, 2018).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of lncRNA-mRNA Interactions in the Non-Small Cell Lung Cancer

Liu¹,

Li²,

Li³

et al. 2020

Preprint

View full text Add to dashboard Cite

As a serious malignancy with high incidence and fatality, non-small cell lung cancer (NSCLC) has affected millions of people each year worldwide. However, the 5-year survival rate of NSCLC is still not satisfactory. Therefore, it is particularly imperative and necessary to explore the underlying molecular mechanisms of NSCLC to contribute to the potential therapeutic targets and strategies. Six clinical samples including three normal tissues and three cancer tissues obtained from patients diagnosed with NSCLC were sequenced through Illumina Hiseq2500 sequencer. Differentially expressed lncRNAs and mRNAs analysis were implemented using the edgeR, and the function of differentially expressed lncRNAs and mRNAs were analyzed with GO and KEGG enrichment analysis. Then, top 10 dysregulated lncRNAs and mRNAs were screened out. Finally, the co-expression, biological function and pathway networks of dysregulated lncRNA with the predicted target genes were constructed. Differentially expressed lncRNAs and mRNAs could distinguish cancerous tissues from normal tissues. The functions and KEGG pathway of differentially expressed lncRNAs and mRNAs mainly were enriched in immune response, metabolism, phagosome, cytokine receptor interaction and IL-17 signaling pathway. The co-expression network revealed that top ten dysregulated lncRNAs were interacted with IGH gene cluster. Furthermore, the biological function and pathway network also indicated that dysregulated lncRNAs were involved in immune process. The results demonstrated that the biologic function of dysregulated lncRNAs that may be involved in immune process. Our data provides a foundation of the biologic significances of lncRNAs to contribute to the therapeutic targets and strategies for NSCLC to promote the survival for patients.

show abstract

Upregulation of LncRNA‐HIT promotes migration and invasion of non‐small cell lung cancer cells by association with ZEB1

Cited by 44 publications

References 29 publications

Long non-coding RNA PICART1 suppresses proliferation and promotes apoptosis in lung cancer cells by inhibiting JAK2/STAT3 signaling

Long non-coding RNA PICART1 suppresses proliferation and promotes apoptosis in lung cancer cells by inhibiting JAK2/STAT3 signaling

LINC‐PINT alleviates lung cancer progression via sponging miR‐543 and inducing PTEN

Transcriptome Analysis of lncRNA-mRNA Interactions in the Non-Small Cell Lung Cancer

Contact Info

Product

Resources

About