Upregulation of Circular RNA circATRNL1 to Sensitize Oral Squamous Cell Carcinoma to Irradiation

Chen, Guanhui; Li, Yiming; He, Yi; Zeng, Binghui; Chen, Yi; Wang, Chao; Zhang, Xiliu; Zhao, Wei; Yu, Dongsheng

doi:10.1016/j.omtn.2019.12.031

Cited by 54 publications

(45 citation statements)

References 52 publications

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“…10 For example, Xia et al reported that a circRNA derived from MMP9 facilitated OSCC metastasis, 11 while Chen et al showed that upregulation of circATRNL1 enhanced the radiosensitivity of OSCC cells. 12 Circ-ITCH, a novel tumor suppressor in many cancers, 13 aroused our great interest. Our study first identified the decreased expression of circ-ITCH in OSCC, whereas low expression of circ-ITCH is closely correlated with malignant progression and poor survival in OSCC patients.…”

Section: Discussionmentioning

confidence: 99%

<p>Circular RNA ITCH Suppresses Cell Proliferation but Induces Apoptosis in Oral Squamous Cell Carcinoma by Regulating miR-421/PDCD4 Axis</p>

Hao¹,

Wangzhou²,

Liang³

et al. 2020

CMAR

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Background: Circular RNAs (circRNAs), a group of covalently closed non-coding RNAs, serve critical regulatory roles in many human cancers, including oral squamous cell carcinoma (OSCC). The purpose of this study was to investigate the functional role of circular RNA ITCH (circ-ITCH) in OSCC and the underlying mechanisms. Methods: RT-qPCR analysis was applied to detect the expression levels of circ-ITCH in OSCC tissues and cell lines. MTT assay and flow cytometer analysis were used to evaluate the effects of circ-ITCH overexpression on the proliferation and apoptosis of OSCC cells. Bioinformatics analysis and dual-luciferase reporter assay were applied to determine the binding relation between circ-ITCH and miR-421 as well as PDCD4 mRNA and miR-421. Results: Our results showed that circ-ITCH expression was remarkably decreased in OSCC tissues and cell lines. Low circ-ITCH expression was strongly associated with adverse clinicopathological characteristics of OSCC patients. Moreover, functional assays demonstrated that circ-ITCH overexpression significantly inhibited OSCC cell proliferation and induced cell apoptosis. Our data further uncovered that circ-ITCH could bind directly to miR-421 and block its repression on PDCD4 in OSCC. MiR-421 expression was significantly increased in OSCC tissues and was inversely correlated with circ-ITCH expression. Notably, miR-421 restoration blocked the tumor-suppressive role of circ-ITCH in OSCC cells. Conclusion: In conclusion, our study reveals that circ-ITCH serves as a tumor suppressor in OSCC partly by regulating miR-421/PDCD4 axis.

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Section: Discussionmentioning

confidence: 99%

<p>Circular RNA ITCH Suppresses Cell Proliferation but Induces Apoptosis in Oral Squamous Cell Carcinoma by Regulating miR-421/PDCD4 Axis</p>

Hao¹,

Wangzhou²,

Liang³

et al. 2020

CMAR

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“…When circATRNL1 is upregulated, the radiosensitivity of OSCC cells is enhanced. This is because it sponges miR-23a-3p to increase the transcription of PTEN and then decreases the phosphorylation of AKT, suggesting that autophagy may be involved [102].…”

Section: Oral Squamous Cell Carcinomamentioning

confidence: 99%

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

et al. 2020

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Circular RNAs (circRNAs), one type of non-coding RNA, were initially misinterpreted as nonfunctional products of pre-mRNA mis-splicing. Currently, circRNAs have been proven to manipulate the functions of diverse molecules, including non-coding RNAs, mRNAs, DNAs and proteins, to regulate cell activities in physiology and pathology. Accumulating evidence indicates that circRNAs play critical roles in tumor genesis, development, and sensitivity to radiation and chemotherapy. Radiotherapy and chemotherapy are two primary types of intervention for most cancers, but their therapeutic efficacies are usually retarded by intrinsic and acquired resistance. Thus, it is urgent to develop new strategies to improve therapeutic responses. To achieve this, clarification of the underlying mechanisms affecting therapeutic responses in cancer is needed. This review summarizes recent progress and mechanisms of circRNAs in cancer resistance to radiation and chemotherapy, and it discusses the limitations of available knowledge and potential future directions.

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“…This effect was dependent on a direct interaction between circATRNL1 and miR-23a as shown by phenotype rescue experiments using miR-23a mimics. This was mediated by the miR-23a target gene PTEN which inactivates the AKT signaling pathway [98].…”

Section: Lncrnasmentioning

confidence: 99%

Non-Coding RNAs in Cancer Radiosensitivity: MicroRNAs and lncRNAs as Regulators of Radiation-Induced Signaling Pathways

Podralska

Ciesielska

Kluiver

et al. 2020

Cancers

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Radiotherapy is a cancer treatment that applies high doses of ionizing radiation to induce cell death, mainly by triggering DNA double-strand breaks. The outcome of radiotherapy greatly depends on radiosensitivity of cancer cells, which is determined by multiple proteins and cellular processes. In this review, we summarize current knowledge on the role of microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), in determining the response to radiation. Non-coding RNAs modulate ionizing radiation response by targeting key signaling pathways, including DNA damage repair, apoptosis, glycolysis, cell cycle arrest, and autophagy. Additionally, we indicate miRNAs and lncRNAs that upon overexpression or inhibition alter cellular radiosensitivity. Current data indicate the potential of using specific non-coding RNAs as modulators of cellular radiosensitivity to improve outcome of radiotherapy.

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Upregulation of Circular RNA circATRNL1 to Sensitize Oral Squamous Cell Carcinoma to Irradiation

Cited by 54 publications

References 52 publications

<p>Circular RNA ITCH Suppresses Cell Proliferation but Induces Apoptosis in Oral Squamous Cell Carcinoma by Regulating miR-421/PDCD4 Axis</p>

<p>Circular RNA ITCH Suppresses Cell Proliferation but Induces Apoptosis in Oral Squamous Cell Carcinoma by Regulating miR-421/PDCD4 Axis</p>

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

Non-Coding RNAs in Cancer Radiosensitivity: MicroRNAs and lncRNAs as Regulators of Radiation-Induced Signaling Pathways

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