Upregulation of a 23kDa small heat shock protein transcript during pupal diapause in the flesh fly, Sarcophaga crassipalpis

Yocum, George D.; Joplin, Karl H.; Denlinger, David L.

doi:10.1016/s0965-1748(98)00046-0

Cited by 155 publications

(109 citation statements)

References 31 publications

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“…In earlier studies, we reported that both a small Hsp (Hsp23) and Hsp70 (now designated as Hsp70A) are highly up-regulated during pupal diapause in S. crassipalpis (17)(18)(19). This developmental upregulation of Hsps occurs simply because the pupa enters diapause and is not a function of temperature stress.…”

Section: Resultsmentioning

confidence: 98%

“…After our initial reports on the diapause up-regulation of Hsp23 and Hsp70 (17,18), Hsps were found to be up-regulated during diapause in a number of additional species, including adult diapause of the Colorado potato beetle, Leptinotarsa decemlineata (24), pupal diapauses of the solitary bee, Megachile rotundata (25) and the onion maggot Delia antiqua (26), larval diapause of the rice stem borer Chilo suppressalis (27), and embryonic diapause of the silkmoth Bombyx mori (28). These published reports, together with our data on five additional species, provide evidence that Hsps are commonly up-regulated during diapause in diverse insect taxa, including Diptera, Lepidoptera, Coleoptera, and Hymenoptera and in diapauses that occur in embryonic, larval, pupal, and adult stages.…”

Section: Resultsmentioning

confidence: 99%

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Up-regulation of heat shock proteins is essential for cold survival during insect diapause

Rinehart

Yocum

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

476

409

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Diapause, the dormancy common to overwintering insects, evokes a unique pattern of gene expression. In the flesh fly, most, but not all, of the fly's heat shock proteins (Hsps) are up-regulated. The diapause up-regulated Hsps include two members of the Hsp70 family, one member of the Hsp60 family (TCP-1), at least four members of the small Hsp family, and a small Hsp pseudogene. Expression of an Hsp70 cognate, Hsc70, is uninfluenced by diapause, and Hsp90 is actually down-regulated during diapause, thus diapause differs from common stress responses that elicit synchronous up-regulation of all Hsps. Up-regulation of the Hsps begins at the onset of diapause, persists throughout the overwintering period, and ceases within hours after the fly receives the signal to reinitiate development. The up-regulation of Hsps appears to be common to diapause in species representing diverse insect orders including Diptera, Lepidoptera, Coleoptera, and Hymenoptera as well as in diapauses that occur in different developmental stages (embryo, larva, pupa, adult). Suppressing expression of Hsp23 and Hsp70 in flies by using RNAi did not alter the decision to enter diapause or the duration of diapause, but it had a profound effect on the pupa's ability to survive low temperatures. We thus propose that up-regulation of Hsps during diapause is a major factor contributing to cold-hardiness of overwintering insects.cold tolerance ͉ overwintering ͉ stress proteins ͉ RNAi

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Up-regulation of heat shock proteins is essential for cold survival during insect diapause

Rinehart

Yocum

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

476

409

View full text Add to dashboard Cite

show abstract

“…pipiens, a small heat shock protein is upregulated in late diapause, but the upregulation is slight by comparison with the strong upregulation of hsp23 noted in S. crassipalpis (Yocum et al, 1998). A slight elevation of hsp70 was also noted in the adult diapause of the Colorado potato beetle (Yocum, 2001), but in adults of Drosophila triauraria, hsps do not appear to be at all upregulated during diapause (Goto and Kimura, 2004).…”

Section: Stress Responsementioning

confidence: 84%

“…These proteins act as molecular chaperones by preventing abnormal protein folding during environmental stresses such as extreme heat, cold, or desiccation and have also been implicated in playing a role in cell cycle arrest (Feder et al, 1992). In the pupal diapause of the flesh fly Sarcophaga crassipalpis, hsp23 and hsp70 are developmentally upregulated upon the entry into diapause and remain expressed until diapause has been broken Yocum et al, 1998), while hsp90 is downregulated at this time but remains responsive to environmental stress .…”

Section: Stress Responsementioning

confidence: 99%

Diapause-specific gene expression in the northern house mosquito, Culex pipiens L., identified by suppressive subtractive hybridization

Robich

Rinehart

Kitchen

et al. 2007

Journal of Insect Physiology

133

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In this study we probe the molecular events underpinning diapause observed in overwintering females of Culex pipiens. Using suppressive subtractive hybridization (SSH) we have identified 40 genes that are either upregulated or downregulated during this seasonal period of dormancy. Northern blot hybridizations have confirmed the expression of 32 of our SSH clones, including 6 genes that are upregulated specifically in early diapause, 17 that are upregulated in late diapause, and 2 upregulated throughout diapause. In addition, 2 genes are diapause downregulated and 5 remain unchanged during diapause. These genes can be categorized into eight functional groups: genes with regulatory functions, metabolically-related genes, those involved in food utilization, stress response genes, cytoskeletal genes, ribosomal genes, transposable elements, and genes with unknown functions.

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“…Studies on sHsp regulation in dormancies of different organisms revealed heterogenous patterns (Bonato et al 1987;Yocum et al 1991;Denlinger et al 1992;Liang et al 1997a;Yocum et al 1998;Tammariello et al 1999;Cherkasova et al 2000;Goto and Kimura 2004;Rinehart et al 2007;Gkouvitsas et al 2008), indicating a diverse array of functions. An essential upregulation of shsp has been suggested for cold hardiness in the flesh fly Sarcophaga crassipalpis (Rinehart et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Stress response in tardigrades: differential gene expression of molecular chaperones

Reuner

Hengherr

Mali

et al. 2010

Cell Stress and Chaperones

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Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (MtsHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.

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Upregulation of a 23kDa small heat shock protein transcript during pupal diapause in the flesh fly, Sarcophaga crassipalpis

Cited by 155 publications

References 31 publications

Up-regulation of heat shock proteins is essential for cold survival during insect diapause

Up-regulation of heat shock proteins is essential for cold survival during insect diapause

Diapause-specific gene expression in the northern house mosquito, Culex pipiens L., identified by suppressive subtractive hybridization

Stress response in tardigrades: differential gene expression of molecular chaperones

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