Upregulating hsa-miR-128a Increased the Effects of Pembrolizumab on Laryngeal Cancer Cells via the p53 Pathway

Chen, Hui; Guo, Yang; Huang, Jiayuan; Zhou, Liang

doi:10.1155/2021/2342784

Cited by 6 publications

(7 citation statements)

References 21 publications

(20 reference statements)

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“…This finding has been supported by study done by Rasmussen et al 2020, that documented significant correlations between IHC biomarkers p53 and PD-L1 (17). Also other study obtained by Hui Chen et al 2021 that refer to this correlation (18).…”

Section: The Correlation Between the Expression Of Pdl1 P53 And Ki67 ...supporting

confidence: 53%

Immunohistochemical expression of PDL1 in laryngeal carcinoma in relation to P53 and ki67 proliferative index

amen

Zwain

Janabi

2022

ijhs

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Squamous cell carcinoma of larynx is one of the most common malignant neoplasms of the head and neck. Carcinoma of the larynx represents 2.2% of all cancers in men and 0.4% in women, with predilection of old aged men of more than 50 years rather than women. Smoking is the main risk factor. Alcohol, nutritional factors and virus as Human papillomavirus (HPV) play a role in the pathogenesis of laryngeal carcinoma. Programmed death-ligand (PDL1) is the new introduced marker that be considered as treatment target nowadays in addition to its prognostic application. Aims of study: This study was designed to assess the predictive the role of PDL1 in laryngeal squamous cell carcinoma in comparison with other prognostic markers (P53 and Ki-67).Results: The current study revealed that PDL1 expression was reported in about 20 % of laryngeal squamous cell carcinoma and 10% in premalignant, while it was negative in benign laryngeal lesions. Others markers; P53 protein was shown to be positive in around 42.5 percent of laryngeal squamous cell carcinomas, 40 percent of premalignant laryngeal lesions, and about 12 percent of benign laryngeal lesions.

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Section: The Correlation Between the Expression Of Pdl1 P53 And Ki67 ...supporting

confidence: 53%

Immunohistochemical expression of PDL1 in laryngeal carcinoma in relation to P53 and ki67 proliferative index

amen

Zwain

Janabi

2022

ijhs

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“…In addition, the role of FOXD2-AS1 in HNSCC was also related to the p53 signaling pathway, and the p53 signaling pathway plays a part in the regulation of expression of several genes and is closely related to the development of human tumors, whose activation initiates cell cycle arrest as well as DNA repair, and inhibits proliferation of OSCC cells [ 38 ]. Overexpression of hsa-miR-128a-mediated p53 signaling pathway increases the role of Pembrolizumab in Laryngeal Squamous Cell Carcinoma Cell lines [ 39 ]. The findings of the current study confirmed that FOXD2-AS1 is elevated in HNSCC tissues and is a potential predictor of HNSCC in patients, thereby promoting the proliferation of HNSCC cell lines.…”

Section: Discussionmentioning

confidence: 99%

Identification and in vitro validation of prognostic lncRNA signature in head and neck squamous cell carcinoma

et al. 2021

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Long non-coding RNAs (lncRNAs) are promising cancer prognostic markers. However, the clinical significance of lncRNA signatures in evaluating overall survival (OS) outcomes of head and neck squamous cell carcinoma (HNSCC) has not been explored. This study aimed to assess the significance of lncRNA in HNSCC and to develop a lncRNA signature related to OS in HNSCC. LncRNA expression matrices were retrieved from the Cancer Genome Atlas (TCGA) data. Least Absolute Shrinkage and Selection of the Operator (LASSO), univariate and multivariate Cox regression were used for establishing a prognostic model. In vitro experiments were carried out to demonstrate the biological role of lncRNA. A prognosis model based on 7 DElncRNAs was finally established.The patients were then divided into high-risk and low-risk groups. Relative to the low-risk group, overall survival times for patients in the high-risk group were significantly low (P=2.466e−07). Risk score remained an independent prognostic factor in univariate (HR=1.329, 95%CI=1.239−1.425, p < 0.001) and multivariate (HR=1.279, 95%CI=1.184−1.382, p < 0.001) Cox regression analyses. The area under the curve (AUC) of the signature was as high as 0.78. Expressions of FOXD2-AS1 in tumor tissues were elevated, and significantly correlated with OS (P=0.008). FOXD2-AS1 silencing then significantly reduced HNSCC cell proliferation, invasion, and migration. In conclusion, a lncRNA signature was established for HNSCC prognostic prediction and FOXD2-AS1 was identified as an HNSCC oncogene.

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“…AMC-HN8 (RRID: CVCL_5966) and TU212 (RRID: CVCL_4915) human LSCC cells [ 20 , 23 – 25 ] were kindly provided by the BeNa Culture Collection (Jiangsu, China). Cells were cultured in high glucose Dulbecco’s modified Eagle medium (DMEM) with 10% foetal bovine serum (PAN-Biotech, Adenbach, Germany) and incubated in a humidified 37 °C incubator with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

A novel tyrosine tRNA-derived fragment, tRFTyr, induces oncogenesis and lactate accumulation in LSCC by interacting with LDHA

et al. 2023

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Background Transfer (t)RNA-derived small RNA (tsRNA), generated from precursor or mature tRNA, is a new type of small non-coding RNA (sncRNA) that has recently been shown to play a vital role in human cancers. However, its role in laryngeal squamous cell carcinoma (LSCC) remains unclear. Methods We elucidated the expression profiles of tsRNAs in four paired LSCC and non-neoplastic tissues by sequencing and verified the sequencing data by quantitative real-time PCR (qRT–PCR) of 60 paired samples. The tyrosine-tRNA derivative tRFTyr was identified as a novel oncogene in LSCC for further study. Loss-of-function experiments were performed to evaluate the roles of tRFTyr in tumorigenesis of LSCC. Mechanistic experiments including RNA pull-down, parallel reaction monitoring (PRM) and RNA immunoprecipitation (RIP) were employed to uncover the regulatory mechanism of tRFTyr in LSCC. Results tRFTyr was significantly upregulated in LSCC samples. Functional assays showed that knockdown of tRFTyr significantly suppressed the progression of LSCC. A series of mechanistic studies revealed that tRFTyr could enhance the phosphorylated level of lactate dehydrogenase A (LDHA) by interacting with it. The activity of LDHA was also activated, which induced lactate accumulation in LSCC cells. Conclusions Our data delineated the landscape of tsRNAs in LSCC and identified the oncogenic role of tRFTyr in LSCC. tRFTyr could promote lactate accumulation and tumour progression in LSCC by binding to LDHA. These findings may aid in the development of new diagnostic biomarkers and provide new insights into therapeutic strategies for LSCC.

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Upregulating hsa-miR-128a Increased the Effects of Pembrolizumab on Laryngeal Cancer Cells via the p53 Pathway

Cited by 6 publications

References 21 publications

Immunohistochemical expression of PDL1 in laryngeal carcinoma in relation to P53 and ki67 proliferative index

Immunohistochemical expression of PDL1 in laryngeal carcinoma in relation to P53 and ki67 proliferative index

Identification and in vitro validation of prognostic lncRNA signature in head and neck squamous cell carcinoma

A novel tyrosine tRNA-derived fragment, tRFTyr, induces oncogenesis and lactate accumulation in LSCC by interacting with LDHA

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