Upregulated protein O-GlcNAcylation promoted functional and structural recovery of the contused spinal cord injury in rats by Thiamet-G treatment

Liang, Hongsheng; Xu, Lin; Gao, Aili; Shao, Yongxiang; Yang, Shanshan; Jiang, Zhenfeng; Ma, Wei; Zhu, Shiyi; Lin, Tie; Zhang, Xiangtong

doi:10.1080/01616412.2019.1611202

Cited by 2 publications

(2 citation statements)

References 43 publications

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“…[167,168] In recent work, the Zhang laboratory found that Thiamet-G treatment reduced lesion size after experimental SCI in rats, promoting spinal cord, and motor function recovery (Table 1). [169] Further studies will be needed to determine the mechanisms underlying these observations. Interestingly, inhibiting OGT may be a useful approach in developing future cell-based therapies for SCI.…”

Section: Amyotrophic Lateral Sclerosis (Als)mentioning

confidence: 99%

Chemical Biology Approaches to Understanding Neuronal O−GlcNAcylation

Huynh

Boyce

2022

Israel Journal of Chemistry

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O-linked β-N-acetylglucosamine (OÀ GlcNAc) is a ubiquitous post-translational modification in mammals, decorating thousands of intracellular proteins. OÀ GlcNAc cycling is an essential regulator of myriad aspects of cell physiology and is dysregulated in numerous human diseases. Notably, OÀ GlcNAcylation is abundant in the brain and numerous studies have linked aberrant OÀ GlcNAc signaling to various neurological conditions. However, the complexity of the nervous system and the dynamic nature of protein OÀ GlcNAcylation have presented challenges for studying of neuronal OÀ GlcNAcylation. In this context, chemical approaches have been a particularly valuable complement to conventional cellular, biochemical, and genetic methods to understand OÀ GlcNAc signaling and to develop future therapeutics. Here we review selected recent examples of how chemical tools have empowered efforts to understand and rationally manipulate OÀ GlcNAcylation in mammalian neurobiology.

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Section: Amyotrophic Lateral Sclerosis (Als)mentioning

confidence: 99%

Chemical Biology Approaches to Understanding Neuronal O−GlcNAcylation

Huynh

Boyce

2022

Israel Journal of Chemistry

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“…injected within 1 h after injury at 10 mg/kg/d for 3 days. This dosing decreased cleaved caspase 3 at 24 h after SCI, decreased CD68 + microglia at 7 days after SCI, improved neurological score at 5–20 days following SCI, and mitigated histological alterations of the injured spinal cord at 24 h and 21 days after SCI ( Liang et al, 2019 ). In a rat kainate acid-induced seizure model, 3 consecutive daily thiamet G injections (10 mg/kg i.p.)…”

Section: Introductionmentioning

confidence: 99%

New Insights Into the Biology of Protein O-GlcNAcylation: Approaches and Observations

et al. 2021

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O-GlcNAcylation is a protein posttranslational modification that results in the addition of O-GlcNAc to Ser/Thr residues. Since its discovery in the 1980s, it has been shown to play an important role in a broad range of cellular functions by modifying nuclear, cytosolic, and mitochondrial proteins. The addition of O-GlcNAc is catalyzed by O-GlcNAc transferase (OGT), and its removal is catalyzed by O-GlcNAcase (OGA). Levels of protein O-GlcNAcylation change in response to nutrient availability and metabolic, oxidative, and proteotoxic stress. OGT and OGA levels, activity, and target engagement are also regulated. Together, this results in adaptive and, on occasions, detrimental responses that affect cellular function and survival, which impact a broad range of pathologies and aging. Over the past several decades, approaches and tools to aid the investigation of the regulation and consequences of protein O-GlcNAcylation have been developed and enhanced. This review is divided into two sections: 1) We will first focus on current standard and advanced technical approaches for assessing enzymatic activities of OGT and OGT, assessing the global and specific protein O-GlcNAcylation and 2) we will summarize in vivo findings of functional consequences of changing protein O-GlcNAcylation, using genetic and pharmacological approaches.

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Upregulated protein O-GlcNAcylation promoted functional and structural recovery of the contused spinal cord injury in rats by Thiamet-G treatment

Cited by 2 publications

References 43 publications

Chemical Biology Approaches to Understanding Neuronal O−GlcNAcylation

Chemical Biology Approaches to Understanding Neuronal O−GlcNAcylation

New Insights Into the Biology of Protein O-GlcNAcylation: Approaches and Observations

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