Upregulated mRNA expression of desaturase and elongase, two enzymes involved in highly unsaturated fatty acids biosynthesis pathways during follicle maturation in zebrafish

Ishak, Sairatul Dahlianis; Tan, Shi Hao; Khong, Hou-Keat; Jaya-Ram, Annette; Enyu, Yee-Ling; Kuah, Meng-Kiat; Shu‐Chien, Alexander Chong

doi:10.1186/1477-7827-6-56

Cited by 19 publications

(13 citation statements)

References 54 publications

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“…In addition, the highest hepatic DHA levels were found in fish fed the diets AV‐EQ2 and AV‐EQ3. Increased LC‐PUFA levels decreased the expression of the delta‐6 desaturase in zebra fish (Ishak et al, ). Thus, a negative feedback loop of DHA could have decreased the expression of fads2a(d6) in the presented study.…”

Section: Discussionmentioning

confidence: 99%

Combination of Dietary Ahiflower Oil and Equol Enhances Long‐Chain Polyunsaturated Fatty Acid Levels in Rainbow Trout Tissues

Fickler

Staats

Michl

et al. 2018

Lipids

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Equol and Ahiflower oil have been shown to increase either eicosapentaenoic acid (EPA, 20:5n‐3) or docosahexaenoic acid (DHA, 22:6n‐3) levels in tissues of rainbow trout when applied individually. Thus, we investigated whether the combination of an Ahiflower oil‐based diet and equol might increase both, EPA and DHA levels, in rainbow trout. Rainbow trout (87.1 ± 0.3 g) were fed with five diets for 8 weeks. A diet based on a blend of fish and vegetable oils (FV) served as a reference diet. The four experimental diets contained a blend of Ahiflower oil and vegetable oils (AV). The AV‐diets were supplemented with equol by 0.0%, 0.1%, 0.2%, and 0.3% DM of the diet (AV‐C, AV‐EQ1, AV‐EQ2, and AV‐EQ3). The dietary treatments did not affect growth performance of fish and chemical nutrient composition of the whole body samples. Fish fed with the equol diets showed dose‐dependently increased liver weights and 18:0 liver levels. The content of EPA showed no consistent pattern between tissues but all AV‐groups were characterized by higher liver EPA values than FV. DHA values of AV‐EQ2 and AV‐EQ3 were similar to FV in fillet, tended to be the highest in the whole body and were significantly higher in liver compared to FV. In contrast, mRNA steady state levels of fatty acyl desaturase 2a (delta‐6) [fads2a(d6)] were not affected by the dietary treatments. In conclusion, the combination of dietary Ahiflower oil and equol (0.2% and 0.3%) seems to affect the fatty acid metabolism of rainbow trout positively to increase DHA tissue levels.

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Section: Discussionmentioning

confidence: 99%

Combination of Dietary Ahiflower Oil and Equol Enhances Long‐Chain Polyunsaturated Fatty Acid Levels in Rainbow Trout Tissues

Fickler

Staats

Michl

et al. 2018

Lipids

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“…The ability of different types of cells to elongate and desaturate n-6 and n-3 fatty acids is diverse (Grammatikos et al 1994b). However, these enzymes have been shown to be expressed in cumulus cells, and are involved in follicular growth and oocyte competence in rat (Moreau et al 2006), human (Feuerstein et al 2007) and zebrafish (Ishak et al 2008). In the mouse, deletion of Fads2 gene expression impaired the gap junction network between granulosa cells of ovarian follicles and resulted in sterility (Stoffel et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Impact of linoleic acid on bovine oocyte maturation and embryo development

Marei¹,

Wathes²,

2010

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Linoleic acid (LA; 18:2 n-6) is the most abundant fatty acid in bovine follicular fluid, and it was previously reported that LA concentration significantly decreases when follicle size increases. This suggests that LA may have a role in the regulation of oocyte maturation. The present study investigated the effect of LA supplementation on bovine oocyte maturation and early embryo development in vitro. Treatment of cumulus-oocyte complexes (COCs) with LA significantly inhibited cumulus cell expansion and retarded development of the oocytes to the metaphase II (MII) stage in a dose-dependent manner. This effect was reversible, and the oocytes developed to the MII stage after extended culture in the absence of LA. Treatment of COCs with LA also resulted in a significantly lower percentage of cleaved embryos and blastocyst yield. Furthermore, COCs treated with LA had significant effects compared with controls in i) increasing prostaglandin E 2 concentration in the medium, ii) decreasing intracellular cAMP at 6 and 24 h of maturation and iii) decreasing phosphorylation of the MAPK1 and 3 at 24 h, and AKT at 6 h of maturation. In conclusion, LA supplementation to bovine oocytes during maturation altered the molecular mechanisms regulating oocyte maturation and resulted in decreased percentage of oocytes at MII stage and inhibition of the subsequent early embryo development. These data provide evidence for adverse effects of LA on oocyte development, which can be associated with dietary increased level of LA in the follicular fluid and the decline in fertility in farm animals and human.Reproduction (2010) 139 979-988

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“…Transcription of elovl and fads could be regulated by developmental stage (Ishak, Tan, Khong, Jaya‐Ram, Enyu, Kuah & Shu‐Chien ; Monroig, Rotllant, Sánchez, Cerdá‐Reverter & Tocher ; Monroig, Rotllant, Cerdá‐Reverter, Dick, Figueras & Tocher ; Tan, Chung & Shu‐Chien ; Morais, Mourente, Ortega, Tocher & Tocher ), environmental factors (salinity and water temperature) (Zheng, Torstensen, Tocher, Dick, Henderson & Bell ; Fonseca‐Madrigal, Pineda‐Delgado, Martinez‐Palacios, Rodriguez & Tocher ) as well as dietary lipid and fatty acid, especially LC‐PUFA according to previous studies on teleosts (Zheng et al . ; Ling, Kuah, Sifzizul, Muhammad, Kolkovski & Shu‐Chien ; Jaya‐Ram, Kuah, Lim, Kolkovski & Shu‐Chien ; Morais et al .…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, tissue distribution and nutritional regulation of a fatty acylelovl5-likeelongase in large yellow croaker,Larimichthys crocea

Zuo

Mai

et al. 2015

Aquac Res

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In this study, the full‐length cDNA of fatty acyl elovl5‐like elongase was first cloned from large yellow croaker, Larimichthys crocea. The cDNA of elovl5‐like was 1551 bp, including a 5′‐terminal untranslated region (UTR) of 120 bp, a 3′‐terminal UTR of 546 bp and an open reading frame (ORF) of 885 bp encoding a polypeptide of 294 amino acid residues. Sequence comparison showed that the predicted protein revealed a high percentage identity (>80%) with Elovl5 from other marine fish species. Tissue distribution analysis revealed that the elovl5‐like was expressed highly in liver, brain and gill, and at much lower levels in stomach, intestine, heart and spleen. Quantity polymerase chain reaction showed that hepatic elovl5‐like transcription decreased significantly with the increase in dietary n‐3LC‐PUFA (P < 0.05). The mRNA levels of elovl5‐like in the liver of fish fed diets with 0.15%, 0.60% and 0.98% n‐3LC‐PUFA were up‐regulated by 1.77‐fold, 1.41‐fold and 1.41‐fold than that with 2.25% n‐3LC‐PUFA respectively. No significant differences were observed in the hepatic mRNA levels of elovl5‐like in response to the increased ratio of dietary DHA/EPA (P > 0.05). These results demonstrate for the first time the presence and nutritional modulation of elovl5‐like cDNA in large yellow croaker. This could contribute to better understanding the process of n‐3LC‐PUFA biosynthesis in this fish species.

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Upregulated mRNA expression of desaturase and elongase, two enzymes involved in highly unsaturated fatty acids biosynthesis pathways during follicle maturation in zebrafish

Cited by 19 publications

References 54 publications

Combination of Dietary Ahiflower Oil and Equol Enhances Long‐Chain Polyunsaturated Fatty Acid Levels in Rainbow Trout Tissues

Combination of Dietary Ahiflower Oil and Equol Enhances Long‐Chain Polyunsaturated Fatty Acid Levels in Rainbow Trout Tissues

Impact of linoleic acid on bovine oocyte maturation and embryo development

Molecular cloning, tissue distribution and nutritional regulation of a fatty acylelovl5-likeelongase in large yellow croaker,Larimichthys crocea

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