Upregulated Expression of Profilin1 on Dendritic Cells in Patients With Severe Aplastic Anemia

J Cellular Molecular Medi

et al. 2022

Self Cite

Cofilin‐1 interacts with actin to regulate cell movement. The importance of cofilin‐1 in immunity has been established, and its involvement in a number of autoimmune diseases has been confirmed. However, its role in severe aplastic anaemia (SAA) remains elusive. Thus, the aim of the current study was to investigate the role of cofilin‐1 in patients with SAA. Flow cytometry, Western blotting and real‐time quantitative reverse transcription‐polymerase chain reaction were performed to detect the mRNA and protein expression of cofilin‐1 in myeloid dendritic cells (mDCs) from patients with SAA. The expression of cofilin‐1 was then suppressed via siRNA, and its effects on mDCs and downstream effector T‐cell function were evaluated. Cofilin‐1 expression was higher in mDCs from patients with SAA and correlated with routine blood and immune indexes. Moreover, cofilin‐1 knockdown in mDCs from patients with SAA reduced their phagocytic capacity, migration capacity, and CD86 expression through F‐actin remodelling, downregulating the stimulatory capacity of mDCs on CD4+ and CD8+ T lymphocytes. Collectively, these findings indicate that cofilin‐1 participates in the hyperfunction of mDCs in patients with SAA and that the downregulation of cofilin‐1 in mDCs from patients with SAA could be a novel treatment approach for SAA.

Section: Discussionsupporting

confidence: 64%

Cofilin‐1 participates in the hyperfunction of myeloid dendritic cells in patients with severe aplastic anaemia

Sun

J Cellular Molecular Medi

et al. 2022

Self Cite

“…In addition, the mechanisms by which the seven signature genes regulate TME and immune response are complex. PFN1 induced tumour metastasis by promoting microvesicle secretion in non‐small cell lung cancer, 57 and increased expression of PFN1 was found in DCs 58 . RHOA/CDC42‐CFL1 axis is critical in mediating the tumour cell migration in lung cancer 59 .…”

Section: Discussionmentioning

confidence: 98%

“…In the IMvigor (UC), GSE91061 (melanoma), GSE35640 (melanoma), GSE78220 (melanoma), Van Allen (melanoma), Nathanson (melanoma), GSE179351 (COAD and PAAD), Braun secretion in non-small cell lung cancer, 57 and increased expression of PFN1 was found in DCs. 58 RHOA/CDC42-CFL1 axis is critical in mediating the tumour cell migration in lung cancer. 59 Direct targeting of HSP90AA1 with daurisoline could destabilize β-catenin to suppress lung cancer tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

“…PFN1 induced tumour metastasis by promoting microvesicle secretion in non‐small cell lung cancer, 57 and increased expression of PFN1 was found in DCs. 58 RHOA/CDC42‐CFL1 axis is critical in mediating the tumour cell migration in lung cancer. 59 Direct targeting of HSP90AA1 with daurisoline could destabilize β‐catenin to suppress lung cancer tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

An artificial intelligence network‐guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms

Han

et al. 2023

Cell Proliferation

The immune cells play an increasingly vital role in influencing the proliferation, progression, and metastasis of lung adenocarcinoma (LUAD) cells. However, the potential of immune cells' specific genes-based model remains largely unknown. In the current study, by analysing single-cell RNA sequencing (scRNA-seq) data and bulk RNA sequencing data, the tumour-infiltrating immune cell (TIIC) associated signature was developed based on a total of 26 machine learning (ML) algorithms. As a result, the TIIC signature score could predict survival outcomes of LUAD patients across five independent datasets. The TIIC signature score showed superior performance to 168 previously established signatures in LUAD. Moreover, the TIIC signature score developed by the immunofluorescence staining of the tissue array of LUAD patients showed a prognostic value. Our research revealed a solid connection between TIIC signature score and tumour immunity as well as metabolism. Additionally, it has been

“…These increased expression levels were positively correlated to the number of immune cells (CD3 + CD8 + ) and cytokines (TNF-α and IFN-γ) in the peripheral blood. 69 The increased expression of pfn1 on MDC likely leads to the hyperfunction of MDC in SAA patients, which causes the upregulation of immune cells and immune factors, and damages bone marrow haematopoietic cells.…”

Section: Increased Phagocytosis Of Myeloid Dendritic Cells In Aa Pati...mentioning

confidence: 99%

Advances in understanding the role of dendritic cells in aplastic anaemia

Gao

2023

Scand J Immunol

Aplastic anaemia (AA) is an autoimmune disease characterized by haematopoietic failure in the bone marrow. Abnormal activation and hyperfunction of T lymphocytes are believed to cause bone marrow damage, which plays a major role in AA pathogenesis. Dendritic cells (DCs) play a vital role in the immune system by processing antigens for presentation to T cells and regulating their differentiation and function. DC dysfunction may cause abnormal T‐cell activation. Recent studies have associated the occurrence and development of AA with DC function. In this review, we have discussed the role of DCs in AA pathogenesis and their potential as putative therapeutic targets for AA.