UPLC-QTOF-MS-guided isolation of anti-COPD ginsenosides from wild ginseng

Zhu, Huayun; Liu, Junli; Lin, Hongqiang; Zhang, Ying; Yang, Na; Zhou, Baisong; Wang, Zhongyao; Hsu, Alan; Liu, Jinping; Li, Pingya

doi:10.1039/c9ra06635g

Cited by 3 publications

(3 citation statements)

References 34 publications

(47 reference statements)

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“…WG refers to ginseng that has been grown in a natural environment for more than 15 years without human intervention. This WG is a substitute for natural WG grown in its original ecological environment [6]. Wang et al [7] used rapid-resolution LC coupled with quadrupole-TOF-MS (RRLC-Q-TOF-MS) to study the nontargeted metabolism in the blood of patients with SQD after ginseng intervention.…”

Section: Introductionmentioning

confidence: 99%

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Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Zhang

et al. 2023

J of Separation Science

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Patients with a spleen-qi deficiency often exhibit dysfunction in the metabolic system. Metabolites are considered the most direct reflection of individual physiological and pathological conditions and represent attractive candidates to provide deep insights into disease phenotypes. This study examines the potential therapeutic mechanism of wild ginseng on spleen-qi deficiency through the analysis of serum and urine metabolomics using rapid-resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. The reasons for the superiority of wild ginseng treatment over cultivated ginseng were also analyzed in depth. After wild ginseng intervention, anandamide, urobilinogen, aldosterone, and testosterone glucuronide were significantly reduced in serum. Meanwhile, argininosuccinic acid, L-cysteine, and seven other metabolites were significantly elevated in serum. Nine metabolites, including Lacetylcarnitine, and citrulline were elevated in the urine, and trimethylamine N-oxide, adrenic acid, and 10 other metabolites were reduced. Arginine biosynthesis, pantothenate and CoA biosynthesis, thiamin metabolism, taurine, and tryptophan metabolism pathways were mainly improved. Further analysis was conducted on the relationship between Lactobacillus and Akkermansia bacteria with metabolites, and it was found that they are mainly related to amino acid metabolites. This study provides strong theoretical support and direction for further explanation of the immune mechanism of wild ginseng and lays the foundation for future studies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Zhang

et al. 2023

J of Separation Science

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“…It has been widely used in analyzing the chemical ingredients of ginseng root, leaf, berry, stem, etc. [ 16 , 17 ]. The UNIFI Scientific Information System is an informatics platform with an embedded Traditional Medicine Library that enables the rapid, comprehensive, and accurate identification and analysis of ingredients in cAR.…”

Section: Introductionmentioning

confidence: 99%

A Qualitative Analysis of Cultured Adventitious Ginseng Root’s Chemical Composition and Immunomodulatory Effects

Chen,

Li,

Chi

et al. 2023

Molecules

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The cultivation of ginseng in fields is time-consuming and labor-intensive. Thus, culturing adventitious ginseng root in vitro constitutes an effective approach to accumulating ginsenosides. In this study, we employed UPLC-QTOF-MS to analyze the composition of the cultured adventitious root (cAR) of ginseng, identifying 60 chemical ingredients. We also investigated the immunomodulatory effect of cAR extract using various mouse models. The results demonstrated that the cAR extract showed significant activity in enhancing the immune response in mice. The mechanism underlying the immunomodulatory effect of cAR was analyzed through network pharmacology analysis, revealing potential ‘key protein targets’, namely TNF, AKT1, IL-6, VEGFA, and IL-1β, affected by potential ‘key components’, namely the ginsenosides PPT, F1, Rh2, CK, and 20(S)-Rg3. The signaling pathways PI3K–Akt, AGE–RAGE, and MAPK may play a vital role in this process.

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Protective effect of total Saponins from American ginseng against cigarette smoke-induced COPD in mice based on integrated metabolomics and network pharmacology

Lin

Wang

et al. 2022

Biomedicine & Pharmacotherapy

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UPLC-QTOF-MS-guided isolation of anti-COPD ginsenosides from wild ginseng

Abstract: Four previously undescribed ginsenosides, along with five known analogues were isolated from wild ginseng by a UPLC-QTOF-MS-guided fractionation procedure.

Cited by 3 publications

References 34 publications

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Urine and serum metabolomics study of wild ginseng in rats with spleen‐qi deficiency using rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

A Qualitative Analysis of Cultured Adventitious Ginseng Root’s Chemical Composition and Immunomodulatory Effects

Protective effect of total Saponins from American ginseng against cigarette smoke-induced COPD in mice based on integrated metabolomics and network pharmacology

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