UPLC‐Q‐TOF/MS‐based metabonomics study on
            <i>Apostichopus Japonicus</i>
            in various aquaculture models

Guo, Chao; Wang, Youquan; Wu, Yanglei; Han, Lingshu; Gao, Chuang; Chang, Yaqing; Ding, Jun

doi:10.1111/are.15729

Cited by 3 publications

(2 citation statements)

References 40 publications

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“…Another research focus has been directed towards the study of metabolomic changes induced by the impact of various factors. LC-MS has been used to highlight metabolomic differences between cage-cultured, pond-cultured, and bottom-sowed A. japonicus [ 176 ]. Multivariate analysis and enrichment of metabolic pathway analyses revealed differential metabolites participating in lipid, amino acid, carbohydrate, and nucleotide metabolism.…”

Section: Applications Of Metabolome-oriented Approaches In Studies Of...mentioning

confidence: 99%

Application of MS-Based Metabolomic Approaches in Analysis of Starfish and Sea Cucumber Bioactive Compounds

2022

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Today, marine natural products are considered one of the main sources of compounds for drug development. Starfish and sea cucumbers are potential sources of natural products of pharmaceutical interest. Among their metabolites, polar steroids, triterpene glycosides, and polar lipids have attracted a great deal of attention; however, studying these compounds by conventional methods is challenging. The application of modern MS-based approaches can help to obtain valuable information about such compounds. This review provides an up-to-date overview of MS-based applications for starfish and sea cucumber bioactive compounds analysis. While describing most characteristic features of MS-based approaches in the context of starfish and sea cucumber metabolites, including sample preparation and MS analysis steps, the present paper mainly focuses on the application of MS-based metabolic profiling of polar steroid compounds, triterpene glycosides, and lipids. The application of MS in metabolomics studies is also outlined.

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Section: Applications Of Metabolome-oriented Approaches In Studies Of...mentioning

confidence: 99%

Application of MS-Based Metabolomic Approaches in Analysis of Starfish and Sea Cucumber Bioactive Compounds

2022

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“…Currently, the main methodologies that are widely applied in metabolomics analysis include high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). Among these analytical techniques, ultraperformance liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) has become an important means for metabolomics studies in multiple areas of sea cucumber research because of its high sensitivity, high separation efficiency, good repeatability and high universality (Li et al, 2019;Zhao et al, 2020;Xing et al, 2021;Guo et al, 2022). However, little is known about the metabolic response in adult A. japonicus across complete natural torpor-arousal cycles.…”

Section: Introductionmentioning

confidence: 99%

Metabolic response of the sea cucumber Apostichopus japonicus during the estivation-arousal cycles

Zhao

Wang

et al. 2022

Front. Mar. Sci.

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Estivation is a widespread survival strategy for dealing with adverse environmental conditions such as high temperature, low oxygen and lack of water or food, which has been reported across multiple vertebrate and invertebrate species. The sea cucumber Apostichopus japonicus is an excellent model organism to investigate the adaptive mechanism of estivation in marine invertebrates. In this study, a metabolomics approach based on ultraperformance liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) was performed to reveal the metabolic response of intestines from adult A. japonicus over the annual estivation-arousal cycle: nonestivation (NA), deep-estivation (DA) and arousal from estivation (AA). A total of 424 metabolites were identified, and among them, 243, 238 and 37 significant differentially metabolites (DMs) were further screened in the comparisons of DA vs. NA, AA vs. DA, and AA vs. NA. Specifically, the levels of metabolites involved in glycolysis and the tricarboxylic acid cycle were significantly decreased, while higher amounts of long-chain fatty acids, phospholipids and free amino acids were found in A. japonicus during estivation, implying that sea cucumbers might reorganize metabolic priorities for ATP production by depressing carbohydrate metabolism and promoting lipid and amino acid catabolism. Interestingly, elevated relative carbon flow entry into the pentose phosphate pathway and accumulation of various nonenzymatic antioxidant molecules (e.g., tocotrienols, folic acid, catechin, genistein and resveratrol) were observed in estivating sea cucumbers, which suggested that enhancement of the reactive oxygen species defense system might promote long-term viability in the hypometabolic state in an energy-efficient manner. Thus, this research provides new insights into the adaptation mechanisms of marine invertebrates to estivation at the metabolic level.

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Genetic diversity and population structure of the endangered Japanese sea cucumber (Apostichopus japonicus) in natural seas of northern China

Wang¹,

Shi²,

Ren³

et al. 2023

Aquaculture Reports

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UPLC‐Q‐TOF/MS‐based metabonomics study on Apostichopus Japonicus in various aquaculture models

Cited by 3 publications

References 40 publications

Application of MS-Based Metabolomic Approaches in Analysis of Starfish and Sea Cucumber Bioactive Compounds

Application of MS-Based Metabolomic Approaches in Analysis of Starfish and Sea Cucumber Bioactive Compounds

Metabolic response of the sea cucumber Apostichopus japonicus during the estivation-arousal cycles

Genetic diversity and population structure of the endangered Japanese sea cucumber (Apostichopus japonicus) in natural seas of northern China

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