UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds

Chernova, Alina; Gubaev, Rim; Mazin, Pavel; Goryunova, S. V.; Demurin, Ya.N.; Горлова, Л.А.; Vanushkina, Anna; Mair, Waltraud; Anikanov, Nikolay; Martynova, Elena; Goryunov, Denis V.; Garkusha, Sergey; Mukhina, Zhanna; Khaytovich, Philipp

doi:10.3390/biom9010009

Cited by 12 publications

(6 citation statements)

References 28 publications

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“…Furthermore, the increase and decrease in TG observed in enterocytes co-cultured with L. paracasei or Llactate and E. coli RB01 or acetate, respectively, was not observed in the jejunum. This discrepancy may reflect intragroup variability and/or an ''overload'' of the small intestine with TG upon gavage with sunflower oil (composed of 97% TG) (Chernova et al, 2018), which may have masked potential differences between groups. However, at circulating level, we observed that plasma TG and/or BODIPY C 12 decreased in mice treated with L. paracasei, E. coli, and their respective effectors, in agreement with the lipid metabolic phenotype they induced at jejunal level.…”

Section: Discussionmentioning

confidence: 99%

Fermentation Products of Commensal Bacteria Alter Enterocyte Lipid Metabolism

Araújo

Tazi

Burlen-Defranoux

et al. 2020

Cell Host & Microbe

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Section: Discussionmentioning

confidence: 99%

Fermentation Products of Commensal Bacteria Alter Enterocyte Lipid Metabolism

Araújo

Tazi

Burlen-Defranoux

et al. 2020

Cell Host & Microbe

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“…Because of its advantages of rapid analysis, high sensitivity, and qualitative and quantitative accuracy, LC–MS has been used in a variety of fields to determine the contents of various nutrients in edible vegetable oils ( 35 – 37 ) and in heated vegetable oils used for frying ( 38 , 39 ). In the present study, we have established for the first time a qualitative and quantitative method for determining six types of FAA in edible vegetable oils.…”

Section: Discussionmentioning

confidence: 99%

Qualitative and Quantitative Analysis of Six Fatty Acid Amides in 11 Edible Vegetable Oils Using Liquid Chromatography–Mass Spectrometry

Dong

Sun

et al. 2022

Front. Nutr.

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Fatty acid amides (FAAs) are endogenous lipid molecules that exhibit various physiological activities. FAAs are usually present at nanomolar levels in biological samples. In this study, a method for the qualitative and quantitative determination of six FAAs (linoleamide, linoleoyl ethanolamide, oleoyl ethanolamide, palmitic amide, oleamide, and octadecanamide) in edible vegetable oils was established. All six FAAs were detected in sesame, peanut, soybean (decolorized and non-decolorized), and blended oils; five in sunflower oil; four in rice oil; three in linseed and olive oils; and two in corn and canola oils. The total contents of FAAs were highest in sesame oil (104.88 ± 3.01 μg/mL), followed by peanut oil (34.96 ± 3.87 μg/mL), soybean oil (16.75 ± 1.27 μg/mL), and blended oil (13.33 ± 0.77 μg/mL), and the contents in the other edible vegetable oils were all <1.03 μg/mL. The concentrations of linoleoyl ethanolamide and oleoyl ethanolamide were highest in non-decolorized soybean oil, while the other four FAAs (linoleamide, palmitic amide, oleamide, and octadecanamide) showed the highest concentrations in sesame oil. The total contents of these FAAs in eight different oils were higher than those in biological fluids and tissue. Our study confirmed that edible vegetable oils are rich in FAAs, and provides reliable data for evaluating the nutritive value of vegetable oils.

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“…Previously we have validated the extraction and pro ling technique for Sun ower FAs [54] and TAGs [55].…”

Section: Uplc-ms Pro Lingmentioning

confidence: 99%

Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content

Chernova

Gubaev

Singh

et al. 2020

Preprint

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Background: Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content in sunflower was under strong selection. Further improvement of the oil properties achieved by modulating its fatty acid composition is one of the main directions in modern oilseed crop breeding. Results: We searched for the genetic basis of fatty acid content variation by genotyping 601 inbred sunflower lines and assessing their lipid and fatty acid composition. Our genome-wide association analysis based on the genotypes of 15,483 SNPs and the concentrations of 23 fatty acids, including minor fatty acids, revealed significant genetic associations for eleven of them. Identified genomic regions included novel loci on chromosomes 3 and 14 cumulatively, explaining up to 34.5% of the total variation of docosanoic acid (22:0) in sunflower oil.Conclusions: This is the first large scale implementation of high-throughput lipidomic profiling on sunflower germplasm characterization. This study contributes to the genetic characterization of Russian collections, which made a substantial contribution to the development of a sunflower as the oilseed crop worldwide and provides new insights into the genetic control of oil composition that can be implemented in future studies.

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UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds

Cited by 12 publications

References 28 publications

Fermentation Products of Commensal Bacteria Alter Enterocyte Lipid Metabolism

Fermentation Products of Commensal Bacteria Alter Enterocyte Lipid Metabolism

Qualitative and Quantitative Analysis of Six Fatty Acid Amides in 11 Edible Vegetable Oils Using Liquid Chromatography–Mass Spectrometry

Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content

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